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Die Kombination von Mikro-Disk-Elektrophorese und Elektroimmundiffusion zur Untersuchung von Kammerwasserproteinen

The combination of micro-disk-electrophoresis and electroimmunodiffusion for investigation of aqueous humor proteins

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Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Aims and scope Submit manuscript

Summary

A combination of micro-disk-electrophoresis and electrophoresis into antibody-containing agarose gel is described which permits both qualitative and quantitative analysis of human aqueous humor proteins. This technique is an enrichment of methods for investigation of aqueous humor.

Zusammenfassung

Es wird die Kombination von Mikro-Disk-Elektrophorese und Elektrophorese in ein antikörperhaltiges Agarosegel beschrieben. Diese Technik erlaubt sowohl eine qualitative als auch eine quantitative Analyse von menschlichen Kammerwasserproteinen und stellt eine Bereicherung der Untersuchungsmethoden des Kammerwassers dar.

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Literatur

  • Clarke, M. H. G., Freeman, T.: Quantitative immunoelectrophoresis of human serum proteins. Clin. Sci.35, 403–413 (1968)

    Google Scholar 

  • Dames, W., Maurer, H. R., Neuhoff, V.: Micro antigen-antibody crossed electrophoresis in vertical agarose gels following micro-disc electrophoresis. Hoppe-Seylers Z. physiol. Chem.353, 554–558 (1972)

    Google Scholar 

  • Dernouchamps, J. P., Vaerman, J. P., Michiels, J., Heremans, J. F.: Techniques de dosage des proteines totales et de diverses especes proteiques de l'humeur aqueuse. Bull. Soc. belge Ophtal.159, 544–554 (1971)

    Google Scholar 

  • Giebel, W., Saechtling, H.: A combination of micro-disc electrophoresis with antigen-antibody crossed electrophoresis. Hoppe-Seylers Z.physiol. Chem.354, 673–681 (1973)

    Google Scholar 

  • Hemmingsen, L., Other, A.: Disk-electrophoresis of aqueous humour. Acta ophthal. (Kbh.)45, 359–370 (1967)

    Google Scholar 

  • Johansson, B. G., Stenflo, J.: Polyacrylamide slab electrophoresis followed by electrophoresis into antibody-containing agarose gel. Analyt. Biochem.40, 232–236 (1971)

    Google Scholar 

  • Krause, U., Raunio, V.: Micro gel electrophoresis. Clin. chim. Acta17, 251–254 (1967)

    Google Scholar 

  • Laurell, C. B.: Antigen-antibody crossed electrophoresis. Analyt. Biochem.10, 358–361 (1965)

    Google Scholar 

  • Meulemans, O.: Determination of total protein in spinal fluid with sulphosalicylic acid and trichloracetic acid. Clin. chim. Acta5, 757–761 (1960)

    Google Scholar 

  • Ressler, N.: Two-dimensional electrophoresis of serum protein antigens in an antibody containing buffer. Clin. chim. Acta5, 795–800 (1960)

    Google Scholar 

  • Schmut, O., Zirm, M.: Die Kombination von Disk-Elektrophorese und Immundiffusion zur Eiweißanalyse des Kammerwassers. Albrecht v. Graefes Arch. klin. exp. Ophthal.188, 217–220 (1973)

    Google Scholar 

  • Schmut, O., Zirm, M.: Vergleichende Untersuchungen über die Normalverteilung der Immun-globuline im menschlichen Serum und Kammerwasser. Klin. Mbl. Augenheilk.164, 368–371 (1974/I)

    Google Scholar 

  • Schmut, O., Zirm, M.: Quantitative Bestimmung verschiedener Kammerwasserproteine. Albrecht v. Graefes Arch. klin. exp. Ophthal.190, 63–67 (1974/II)

    Google Scholar 

  • Schmut, O., Zirm, M.: Immunologische Bestimmung von Lipoproteinen im Kammerwasser. Albrecht v. Graefes Arch. klin. exp. Ophthal.191, 19–23 (1974/III)

    Google Scholar 

  • Svendsen, P. J., Axelsen, N. H.: A modified antigen-antibody crossed electrophoresis characterizing the specificity and titre of human precipitins against “Candida albicans”. J. Immunol. Meth.1, 169–176 (1972)

    Google Scholar 

  • Zirm, M., Petek, W., Schmut, O., Hofmann, H., Holasek, A.: Quantitative Bestimmung von Kammerwasserproteinen durch die Elektroimmundiffusion in einem antiserumhaltigen Agarosegel. Albrecht v. Graefes Arch. klin. exp. Ophthal.196, 127–132 (1975)

    Google Scholar 

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Schmut, O., Zirm, M. Die Kombination von Mikro-Disk-Elektrophorese und Elektroimmundiffusion zur Untersuchung von Kammerwasserproteinen. Albrecht von Graefes Arch. Klin. Ophthalmol. 199, 69–74 (1976). https://doi.org/10.1007/BF00660816

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  • DOI: https://doi.org/10.1007/BF00660816

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