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Theory of gel filtration chromatography. Elution patterns of solutes in the presence of micellar and premicellar aggregates of micelle-forming surfactants

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Abstract

The theory presented prevously by us (J. Am. Chem. Soc., 100 (1978) 5914) of Gel Filtration Chromatography (GFC) with varying concentration of the aqueous surfactant solutions and fixed solute concentration has been augmented. The rationalisation of our GFC results with the CTAB surfactant solutions and Co(cydta)− on the Sephadex G-25 fine columns demanded the postulation of the premicellar aggregate formation. The GFC theory has been worked out using these observations. Four different distinguished possibilities in the realm of the premicellar aggregation have been investigated. The use of the equations so developed for the elution patterns of the solutes with negative and positive adsorption on the gel matrix has been discussed. Moreover, the presence of the premicellar aggregates in the imbibed phase beyond the usual CMC modifies the relevant GFC expression originally derived by Herries, Bishop and Richards (J. Phys. Chem., 68 (1964) 1842).

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Author notes

  1. A. A. Bhalekar

    Present address: Department of Chemistry, Nagpur University, Amravati Road The Campus, 440 010, Nagpur, India

Authors and Affiliations

  1. Department of Organic Chemistry, The University, Zernikelaan, Groningen, The Netherlands

    J. B. F. N. Engberts

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  1. A. A. Bhalekar
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  2. J. B. F. N. Engberts
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Bhalekar, A.A., Engberts, J.B.F.N. Theory of gel filtration chromatography. Elution patterns of solutes in the presence of micellar and premicellar aggregates of micelle-forming surfactants. Colloid Polym Sci 271, 1068–1075 (1993). https://doi.org/10.1007/BF00659296

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  • DOI: https://doi.org/10.1007/BF00659296

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