Abstract
The interaction of acidic capsular polysaccharide isolated fromKlebsiella K26 with cationic dyes pinacyanol chloride, acridine orange and phenosafranin has been studied by spectral measurements. The polymer induces metachromasy in pinacyanol chloride dye, indicating a blue shift in the visibly absorption spectrum of the dye from 600 to 500 nm. The stoichiometry of polymer/dye in the metachromatic compound, thermodynamic parameters of interaction, and effects of different cosolvents on the stability of the complex have been studied. Spectrophotometric and spectrofluorometric properties of the interaction between the polymer and all three dyes are presented. The chromotropic property of the polymer has been established.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nimmich W, Wünter W (1967) Experentia 23:907
Difabio J, Dutton GGS (1981) Carbohydr Res 92:287
Michalis L, Granick S (1945) J Am Chem Soc 67:1212
Pal MK, Schubert M (1961) J Histochem Cytochem 9:673
Young MD, Phillips GO, Balazs EA (1967) Biochem Biophys Acta 141:374
Stone AL, Bradley DF (1967) Biochim Biophys Acta 148:172
Chakrabarti A, Nath RK, Chakraborty AK (1987) Ind J Biochem Biophys 24:229
Chakrabarti A, Nath RK, Chakraborty AK (1989) Spectrochim Acta 45A:981
Mitra A, Chakrabarti A, Nath RK, Chakraborty AK (1990) Ind J Biochem Biophys 27:291
Westphal O, Jann K (1965) Methods Carbohydr Chem 5:83
MacIntosh FC (1941) Biochem J 35:776
Pal MK, Ghosh BK (1980) Makromol Chem 181:1459
Basu S, Gupta AK, Rohatgi-Mukherjee (1982) J Ind Chem Soc 59:578
West W, Pearce S (1965) J Phy Chem 69(6):1894
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mitra, A., Nath, R.K. & Chakraborty, A.K. Interaction of bacterial acidic polysaccharide with cationic dyes. Colloid Polym Sci 271, 1042–1048 (1993). https://doi.org/10.1007/BF00659293
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00659293