Colloid and Polymer Science

, Volume 270, Issue 9, pp 901–911 | Cite as

Behavior of pure and mixed DPPC liposomes spread or adsorbed at the air-water interface

  • M. A. Launois-Surpas
  • Tz. Ivanova
  • I. Panaiotov
  • J. E. Proust
  • F. Puisieux
  • G. Georgiev
Original Contributions


Liposomes from pure dipalmitoylphosphatidylcholine (DPPC) and mixed DPPC: distearoylphosphatidylcholine (DSPC): soybean lecithin (SL) prepared by the Bangham method with sonication were dispersed into solution or spread at the interface and the kinetics of the surface film formation was studied by measuring and recording the evolution of superficial tension, surface potential, and superficial (14C labeled) DPPC density.

A simple theoretical approach can describe these kinetics by two processes: irreversible diffusion of closed vesicles into or from the bulk phase, and irrevers ible transformation of closed spherical vesicles into destroyed ones which form the surface film. Diffusion controls the phenomenon for small initial amounts of liposomes.

Transformation controls the phenomenon for important initial amounts of liposomes. The kinetic constant of the transformation,K, does not depend on the technique used to form the surface film (spreading or adsorption).

The equilibrium and rheological properties of surface films formed after liposome spreading are compared to those of monolayers

Key Words

Liposomes adsorption spreading surfacefilm monolayer 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Von Neergaard K (1929) Z Ges Exp Med 66:373Google Scholar
  2. 2.
    Clements JA (1977) Amer Rev Respir Dis 115:67Google Scholar
  3. 3.
    Pattus F, Desnuelle P, Verger R (1978) Biochim Biophys Acta 507:62Google Scholar
  4. 4.
    Obladen M, Popp D, Scholl C, Schwartz H, Jähnig F (1983) Biochim Biophys Acta 735:215Google Scholar
  5. 5.
    Schindler H (1979) Biochim Biophys Acta 555:316Google Scholar
  6. 6.
    Ivanova Tz, Georgiev G, Panaiotov I, Ivanova M, Launois-Surpas MA, Proust JE, Puisieux F (1989) Prog Colloid Polym Sci 79:23Google Scholar
  7. 7.
    Bonte F, Taupin C, Puisieux F (1987) J Coll Interface Sci 115:268Google Scholar
  8. 8.
    De Fontages A, Bonte F, Taupin C, Ober R (1985) Colloids Surfaces 14:309Google Scholar
  9. 9.
    Bonte F, Dehan M, Le Ridant A, Puisieux F, Brevet Français No 85. 12950, ADIR and Co., Neuilly-sur-Seine, France, 1985Google Scholar
  10. 10.
    Bangham AD, Standish MM, Watkins JC (1965) J Mol Biol 13:238Google Scholar
  11. 11.
    Takayama M, Itoh S, Nagasaki T, Tanimizu I (1977) Clin Chim Acta 79:93Google Scholar
  12. 12.
    Bangham AD, Horne RW (1964) J Mol Biol 8:660Google Scholar
  13. 13.
    De Haulme M, Hendricx Y, Ter-Minassian Saraga L (1968) J Chim Phys 66:653Google Scholar
  14. 14.
    Proust JE, Baszkin A, Boissonnade MM (1983) J Colloid Interface Sci 94:421Google Scholar
  15. 15.
    Baszkin A, Deyme M, Perez E, Proust JE (1987) In: Brasch JL, Herbett TA (eds.) ASC. Symposium Serie, 343, 451Google Scholar
  16. 16.
    Dimitrov DS, Panaiotov I (1975–76) Ann Univ Sofia, Fac Chim 70(1):103Google Scholar
  17. 17.
    Panaiotov I, Dimitrov DS, Georgiev G (1980) Studia Biophys 78:95Google Scholar
  18. 18.
    Dimitrov DS, Panaiotov I, Richmond P, Ter-Minassian-Saraga L (1978) J Coll Interface Sci 65:483Google Scholar
  19. 19.
    Panaitov I, Dimitrov DS, Ter-Minassian-Saraga L (1979) J Coll Interface Sci 72:49Google Scholar
  20. 20.
    Vassiliev CS, Montchilova IB, Panaiotov I, Ivanova Tz, 7th. ICSCS Symposium A, No 0170, Compiègne France, 10–12 juillet 1991Google Scholar
  21. 21.
    Ward AFH, Tordai Z (1946) J Chem Phys 14:453Google Scholar
  22. 22.
    Ivanova Tz, Panaiotov I, Georgiev G, Launois-Surpas MA, Proust JE, Puisieux F (1991) Colloid and Surfaces Sciences 60:263Google Scholar
  23. 23.
    Gershfeld NL, Stevens WF, Nossal RJ (1985) Biophys J 47:203Google Scholar
  24. 24.
    Gershfeld NL, Stevens WF, Nossal RJ (1986) Faraday Discussion Chemical Soc 81:19Google Scholar
  25. 25.
    Tchoreloff P, Denizot B, Proust JE, Puisieux F (1990) Progress in Respiration Research 25:168Google Scholar
  26. 26.
    Belorgey O, Tchoreloff P, Benattar JJ, Proust JE (1991) J colloid Interface Sci 146:373Google Scholar
  27. 27.
    Tchoreloff P, Gulik A, Proust JE, Puisieux F (1991) Chemistry and Physics of Lipids 59:151Google Scholar

Copyright information

© Steinkopff-Verlag 1992

Authors and Affiliations

  • M. A. Launois-Surpas
    • 1
  • Tz. Ivanova
    • 3
  • I. Panaiotov
    • 4
  • J. E. Proust
    • 2
  • F. Puisieux
    • 1
  • G. Georgiev
    • 3
  1. 1.Physicochimie des Surfaces et Innovation en PharmacotechnieCNRS UA 1218Chatenay-MalabryFrance
  2. 2.Laboratoire de Physique, Faculté de PharmacieAngersFrance
  3. 3.Pulma LaboratorySofiaBulgaria
  4. 4.Physical Chemistry DepartmentUniversity of SofiaSofiaBulgaria

Personalised recommendations