Pflügers Archiv

, Volume 398, Issue 3, pp 236–240 | Cite as

The influence of catecholamines on Na, K transport in slow- and fast-twitch muscles of the rat

  • György Pfliegler
  • Imre Szabó
  • Tibor Kovács


The effects of catecholamines on active sodium and potassium transport was compared in slow- (SOL) and fasttwitch (EDL) skeletal muscles of the rat. Stimulation of active Na+-extrusion and K+-uptake induced by adrenaline (6–30 μmol · 1−1) and isoprenaline (1–40 μmol · l−1) was markedly greater in slow- than in fast-twitch muscle. In sodiumpreloaded muscles the maximal stimulation of24Na-efflux induced by adrenaline was about 2-fold higher in SOL than in EDL. Isoprenaline caused a 2.4-fold increase in ouabainsensitive86Rb influx in SOL muscle, but failed to alter the ouabain-sensitive influx in EDL. The stimulating action of isoprenaline on86Rb influx in EDL was due to an increase in the ouabain-insensitive fraction of Rb uptake. The effects of catecholamines of fast- and slow-twitch muscles were probably due to the accumulation of cyclic AMP, however the fact that there were no significant differences between the nucleotides levels in fast- and slow-twitch muscle suggests the participation of other mechanism as well. The results presented suggest that cyclic AMP-induced stimulation of ouabain-insensitive transport of cation in the isolated EDL muscle of the rat is similar to that of barnacle muscle.

Key words

Slow- and fast-twitch muscles Quabainsensitive and ouabain-insensitive86Rb influx 24Na and42K transport 


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  1. 1.
    Al-Jeboory AA, Marshall RJ (1978) Correlation between the effects of salbutamol on concentrations and cyclic AMP content of isolated fast- and slow-contracting muscles of guinea pig. Naunyn-Schmiedeberg's Arch Pharmacol 305:201–206Google Scholar
  2. 2.
    Ashby CD, Walsh DA (1972) Characterization of the interaction of protein inhibitor with adenosine 3′,5′-monophosphate-dependent protein kinases. I. Interaction with the catalytic subunit of the protein kinase. J Biol Chem 247:6637–6642Google Scholar
  3. 3.
    Bittar EE, Chambers G, Schultz R (1976) Mode of stimulation by adenosine 3′:5′-cyclic monophosphate of the sodium efflux in barnacle muscle fibres. J Physiol (Lond) 257:561–579Google Scholar
  4. 4.
    Bowman WC, Zaimis E (1958) The effects of adrenaline, noradrenaline and isoprenaline on skeletal muscle contractions in the cat. J Physiol (Lond) 144:92–107Google Scholar
  5. 5.
    Bowman WC, Nott MW (1969) Actions of sympathomimetic amines and their antagonists on skeletal muscle. Pharmacol Rev 21:27–72Google Scholar
  6. 6.
    Bowman WC, Raper C (1967) Adrenotropic receptors in skeletal muscle. Ann NY Acad Sci 139:741–753Google Scholar
  7. 7.
    Caldwell PC, Keynes RD (1969) The exchange of22Na between frog sartorius muscle and the bathing medium. In: Passow H, Stampfli R (eds) Laboratory techniques in membrane biophysics. Springer, Berlin Heidelberg New York, pp 63–68Google Scholar
  8. 8.
    Clausen T, Flatman JA (1977) The effect of catecholamines on Na-K-transport and membrane potential in rat soleus muscle. J Physiol (Lond) 270:383–414Google Scholar
  9. 9.
    Clausen T, Flatman JA (1980) β2-adrenoceptors mediate the stimulating effect of adrenaline on active electrogenic Na-K-transport in rat soleus muscle. Br J Pharmacol 68:749–755Google Scholar
  10. 10.
    Dockry M, Kernan RP, Tangney A (1966) Active transport of sodium and potassium in mammalian skeletal muscle and its modification by nerve and by cholinergic and adrenergic agents. J Physiol (Lond) 186:187–200Google Scholar
  11. 11.
    Evans RH, Smith JW (1973) Mode of action of catecholamines on skeletal muscle. J Physiol (Lond) 232:81PGoogle Scholar
  12. 12.
    Fellenius E, Hedberg R, Holmberg E, Waldeck B (1980) Functional and metabolic effects of terbutaline and propranolol in fast- and slow-contracting skeletal muscle in vitro. Acta Physiol Scand 109:89–95Google Scholar
  13. 13.
    Flatman JA, Clausen T (1979) Combined effects of adrenaline and insulin on active electrogenic Na+−K+ transport in rat soleus muscle. Nature (Lond) 281:580–581Google Scholar
  14. 14.
    Hays ET, Dwyer TM, Horowicz P, Swift JG (1974) Epinephrine action on sodium fluxes in frog striated muscle. Am J Physiol 227:1340–1374Google Scholar
  15. 15.
    Pfliegler Gy, Kovács T, Szabó B (1981) The inhibitory actions of eserine and ouabain on the K, Rb, and Cs uptake in slow- and fasttwitch muscles of the rat. Acta Physiol Acad Sci Hung 57:317–328Google Scholar
  16. 16.
    Reddy NB, Oliver KL, Engel WK (1979) Differences in catecholamine-sensitive adenylate cyclase and β-adrenergic receptor binding between fast-twitch and slow-twitch skeletal muscle membranes. Life Sciences 24:1765–1772Google Scholar
  17. 17.
    Rogus EM, Cheng LC, Zierler K (1977) β-Adrenergic effect on Na+−K+ transport in rat skeletal muscle. Biochim Biophys Acta 464:347–355Google Scholar
  18. 18.
    Schulze W, Wollenberger A (1976) Zur Lokalisation der Adenylatzyklase im roten und weißen Skelettmuskel: Eine zytochemische Untersuchung. Acta Biol Med Germ 35:837–843Google Scholar
  19. 19.
    Severson DL, Drummond GI, Sulakhe PV (1972) Adenylate cyclase in skeletal muscle. Kinetic properties and hormonal stimulation. J Biol Chem 247:2949–2958Google Scholar
  20. 20.
    Tashiro N (1973) Effects of isoprenaline on contractions of directly stimulated fast and slow skeletal muscles on the guinea pigs. Br J Pharmacol 48:113–122Google Scholar
  21. 21.
    Waldeck B (1979) β-Adrenoceptors in skeletal muscle. Acta Pharmacol Toxicol (Suppl) 44:28–30Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • György Pfliegler
    • 1
  • Imre Szabó
    • 1
  • Tibor Kovács
    • 1
  1. 1.Department of PhysiologyUniversity Medical SchoolDebrecenHungary

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