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Properties of an inward rectifying K channel in the membrane of guinea-pig atrial cardioballs

  • Excitable Tissues and Central Nervous Physiology
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Abstract

Single channel outward current fluctuations are recorded in excised (outside-out) membrane patches of isolated atrial cells in culture (cardioballs) from hearts of adult guinea-pigs. The ionic channel displays a high selectivity to K ions. Accordingly the reversal potential of the single channel current is close to the K equilibrium potential. The open channel conductance is unaffected by the membrane potential but depends on the K concentration of the outside solution (19.7pS at 2 mM K0 to 30.7pS at 20 mM K0). The open state probability (P0) of the channel shows a marked voltage dependence. P0 amounts to c.0.9 at −40mV and decreases to c.0.1 at +40mV.

Under the assumption of no channel interaction a macroscopic steady state current voltage relationship is reconstructed from the single channel data. The relationship displays inward-going rectification. The rectification is due to the voltage dependence of P0. TheI–V curve displays a negative slope at membrane potentials positive to −15 mV.

In bathing solutions containing Ba ions (0.2 mM) P0 is reduced by rapid closures which interrupt the open state events. The unit channel conductance is unaffected by Ba ions. The channel block exerted by Ba ions is augmented with increasing membrane hyperpolarization.

The results suggest that the channel studied may represent a background K conductance.

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Authors and Affiliations

  1. Institut für Zellphysiologie der Ruhr-Universität Bochum, Postfach 102148, D-4630, Bochum, Federal Republic of Germany

    M. Bechem, H. G. Glitsch & L. Pott

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  1. M. Bechem
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  2. H. G. Glitsch
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  3. L. Pott
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Additional information

This work was supported by a grant to L.P. from the Ministerium für Wissenschaft und Forschung des Landes Nordrhein-Westfalen (AZ.IV B5-FA 8833)

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Bechem, M., Glitsch, H.G. & Pott, L. Properties of an inward rectifying K channel in the membrane of guinea-pig atrial cardioballs. Pflugers Arch. 399, 186–193 (1983). https://doi.org/10.1007/BF00656713

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  • DOI: https://doi.org/10.1007/BF00656713

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