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Production of xylanases byAspergillus fumigatus andAspergillus oryzae on xylan-based media

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Abstract

Aspergillus fumigatus andA. oryzae were cultivated in laboratory fermenters on media containing xylan as the main carbon source.A. fumigatus produced xylanase on unsubstituted, insoluble beech xylan but growth and enzyme production on soluble xylo-oligosaccharides from the steaming of hardwood were poor due to the presence of inhibitors. An essential prerequisite for good xylanase production byA. fumigatus was decrease in the pH of the cultivation below 3.0 At higher pH values, the production of proteolytic enzymes caused degradation of the xylanase activity already produced.A. oryzae produced rather less xylanase activity thanA. fumigatus on the beech xylan medium but, after adaptation, was capable of efficient enzyme production on the steamed substrate.

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References

  • Bailey, M.J., Biely, P. & Poutanen, K. 1992 Interlaboratory testing of methods for assay of xylanase activity.Journal of Biotechnology 23, 257–270.

    Google Scholar 

  • Bailey, M.J. & Poutanen, K. 1989 Production of xylanolytic enzymes by strains ofAspergillus.Applied Microbiology and Biotechnology 30, 5–10.

    Google Scholar 

  • Bailey, M.J. & Siika-aho, M. 1988 Production of microbial rennin.Biotechnology Letters 10, 161–166.

    Google Scholar 

  • Biely, P. 1985 Microbial xylanolytic systems.Trends in Biotechnology 3, 286–290.

    Google Scholar 

  • Buchert, J., Niemelä, K., Puls, J. & Poutanen, K. 1990 Improvement in the fermentability of steamed hemicellulose hydrolyzates by ion exclusion.Process Biochemistry International 25, 176–180.

    Google Scholar 

  • International Union of Pure and Applied Chemistry 1987 Measurement of cellulase activities.Pure and Applied Chemistry 59, 257–268.

    Google Scholar 

  • Kantelinen, A., Rättö, M., Sundqvist, J., Ranua, M., Viikari, L. & Linko, M. 1988 Hemicellulases and their potential role in bleaching. In1988 International Pulp Bleaching Conference, Tappi Proceedings, pp. 1–9, Atlanta.

  • Kantelinen, A., Sundqvist, J., Linko, M. & Viikari, L. 1991 The role of reprecipitated xylan in the enzymatic bleaching of kraft pulp. InProceedings of the 6th International Symposium on Wood and Pulping Chemistry, Vol. 1, pp. 493–500, Melbourne.

  • Lenz, J. & Schurz, J. 1986 Neue Wege zur Verwertung von Hemicellulosen.Österreichische Chemie 11, 318–321.

    Google Scholar 

  • Paice, M., Bernier, M. & Jurasek, L. 1988 Viscosity enhancing bleaching of hardwood kraft pulp with xylanase from a cloned gene.Biotechnology and Bioengineering 32, 235–239.

    Google Scholar 

  • Puls, J., Poutanen, K., Körner, H.-U. & Viikari, L. 1985 Biotechnical utilization of wood carbohydrates after steaming pretreatment.Applied Microbiology and Biotechnology 22, 416–423.

    Google Scholar 

  • Suihko, M.-L. 1989 VTT collection of industrial microorganisms. InCatalogue of Strains 2nd edn. Espoo, Finland: VTT, Biotechnical Laboratory.

    Google Scholar 

  • Sumner, J.B. & Somers, G.F. 1949. Dinitrosalicylic acid for glucose. InLaboratory Experiments in Biological Chemistry, 2nd edn, pp. 38–39. New York: Academic Press.

    Google Scholar 

  • Viikari, L., Kantelinen, A., Rättö, M. & Sundqvist, J. 1991b Enzymes in pulp and paper processing. InEnzymes in Biomass Conversion, eds Leatham, G.F. & Himmel, M.E. pp. 12–22. Washington, DC: Amrican Chemical Society.

    Google Scholar 

  • Viikari, L., Ranua, M., Kantelinen, A., Linko, M. & Sundqvist, J. 1986 Bleaching with enzymes. InProceedings of the 3rd International Conference on Biotechnology in the Pulp and Paper Industry, pp. 67–69. Stockholm.

  • Viikari, L., Ranua, M., Kantelinen, A., Linko, M. & Sundqvist, J. 1987 Application of enzymes in bleaching. InProceedings of the 4th International Symposium on Wood and Pulping Chemistry, Vol. 1, pp. 151–154. Paris.

  • Viikari, L., Sundqvist, J. & Kettunen, J. 1991a Xylanase enzymes promote pulp bleaching.Paper and Timber 73, 384–389.

    Google Scholar 

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M.J. Bailey and L. Viikari are with the VTT, Biotechnical Laboratory, PO Box 202, SF-02151 Espoo, Finland

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Bailey, M.J., Viikari, L. Production of xylanases byAspergillus fumigatus andAspergillus oryzae on xylan-based media. World J Microbiol Biotechnol 9, 80–84 (1993). https://doi.org/10.1007/BF00656523

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