Skip to main content
SpringerLink
Log in

Production of α-amylase fromHalobacterium halobium

  • Research Papers
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Maximum production of extracellular α-amylase activity inHalobacterium halobium was at 40°C in a medium containing 25% (w/v) NaCl, 1% (w/v) soluble starch and 1% (w/v) peptone, in presence of 0.1mm ZnSO4 after 5 days in shaking cultures. The amylase had optimal activity at pH 6.5 in the presence of 1 to 3% (w/v) NaCl at 53°C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Danson, M.J. 1988 Archaebacteria: the comparative enzymology of their central metabolic pathways.Advances in Microbial Physiology 29, 165–231.

    Google Scholar 

  • Denault, L.J. & Underkofler, L.A. 1963 Conversion of starch by microbial enzymes for production of syrups and sugars.Cereal Chemistry 40, 618–629.

    Google Scholar 

  • Eisenberg, H. & Wachtel, E.J. 1987 Structural studies of halophilic proteins, ribosomes and organelles of bacteria adapted to extreme salt concentrations.Annual Reviews in Biophysics and Biophysiological Chemistry 16, 69–92.

    Google Scholar 

  • Good, W.A. & Hartman, P.A. 1970 Properties of the amylase fromHalobacterium halobium.Journal of Bacteriology 104, 601–603.

    Google Scholar 

  • Hough, D.W. & Danson, M.J. 1989 Archaebacteria: ancient organisms with commercial potential.Letters in Applied Microbiology 9, 33–39.

    Google Scholar 

  • Komaki, T. 1956 Industrial applications of amylase.Denpum Kogyo Gakkaishi 4, 1–8.

    Google Scholar 

  • Kushner, D.J. 1985 The Halobacteriaceae. In:The Bacteria Vol. 8, eds. Woese, C.R. & Wolf, R.S.; pp. 171–214. London: Academic Press.

    Google Scholar 

  • Miller, G.L. 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugars.Analytical Chemistry 31, 426–428.

    Google Scholar 

  • Oesterhelt, D. & Krippahl, G. 1983 Phototrophic growth of Halobacteria and its use for isolation of photosynthetically deficient mutants.Annals of Microbiology 134, 137–150.

    Google Scholar 

  • Zaccai, G. & Eisenberg, H. 1990 Halophilic proteins and the influence of solvent on protein stabilization.Trends in Biochemical Sciences 15, 333–337.

    Google Scholar 

Download references

Authors
  1. S. Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Madamwar
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

S. Patel, N. Jain and D. Madamwar are with the Post Graduate Department of Biosciences, Sadar Patel University, Vallabh Vidyanagar-388120, India.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Patel, S., Jain, N. & Madamwar, D. Production of α-amylase fromHalobacterium halobium . World J Microbiol Biotechnol 9, 25–28 (1993). https://doi.org/10.1007/BF00656510

Download citation

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00656510

Key words

Search

Navigation