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Colloid and Polymer Science

, Volume 274, Issue 6, pp 513–519 | Cite as

Atomic force microscopy of human serum albumin (HSA) on poly(styrene/acrolein) microspheres

  • D. Kowalczyk
  • J. -P. Marsault
  • S. Slomkowski
Original Contribution

Abstract

Atomic Force Microscopy (AFM) in the tapping mode was used for the observation of bare poly (styrene/acrolein) P(SA) microspheres and microspheres with attached HSA. Prior to the AFM observations the P(SA) microspheres were immobilized covalently on the surface of quartz slides modified with γ-aminopropyltriethoxysilane. Atomic Force Microscopy pictures were registered for the dry samples. The partial coalescence of the P(SA) microspheres connected to the quartz surface with amino groups has been observed. The AFM pictures of the single P(SA) microspheres revealed that the surface of these particles is smooth and that any irregularities, if present, do not exceed 1 nm. The surface of microspheres with attached HSA has very clearly different morphology with regular pattern of HSA macromolecules. Cracks on the surfaces of some microspheres with HSA revealed that protein macromolecules are attached to these particles in several layers. In the case of some other microspheres the defects in protein attachment allowed the observation of the border between the bare surface of the P(SA) microspheres and the surface covered with protein macromolecules. Comparison of the thickness of the HSA layers on the P(SA) microspheres with the dimensions of HSA macromolecules, determined earlier from the x-ray studies, suggests that the first layer, 3.0±0.2 nm thick, is formed of the HSA macromolecules arranged flatly on the surface whereas protein macromolecules in the subsequent layers, each 8.6±1 nm thick, are adsorbed protruding from the surface.

Key words

Atomic force microscopy human serum albumin poly (styrene/acrolein) microspheres 

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References

  1. 1.
    Szycher M (ed) (1991) High Performance Biomaterials. A comprehensive Guide to Medical and Pharmaceutical Applications, Technomic Publishing Co, Lancaster, BaselGoogle Scholar
  2. 2.
    Rosoff M (ed) (1989) Controlled Release of Drugs: Polymers and Aggregate Systems. VCH Publishers, New YorkGoogle Scholar
  3. 3.
    Rembaum A, Yen SPS, Molday RS (1979) J Macromol Sci, Chem A 13:603Google Scholar
  4. 4.
    Guiot P, Couvreur P (eds) (1986) Polymeric Nanoparticles and Microspheres. CRC Press, Boca Raton, FLGoogle Scholar
  5. 5.
    Rembaum A, Tökés ZA (eds) (1988) Microspheres: Medical and Biological Applications. CRC Press, Boca Raton, FLGoogle Scholar
  6. 6.
    Piskin E, Tuncel A, Denizli A, Ayhan H (1994) J Biomater Sci Polym Edn 5:451Google Scholar
  7. 7.
    Coliwick SP, Kaplan NO (eds) (1987) Immobilized Enzymes and Cells, Part C, Vol 136 In: Mosbach K (series ed) Methods in Enzymology, Academic Press, OrlandoGoogle Scholar
  8. 8.
    Andrade JD, Hlady V (1986) Adv Polym Sci 79:1Google Scholar
  9. 9.
    Lundström I, Ivarsson B, Jönsson U, Elwing H (1987) In: Feast WJ, Munro HS (eds) Polymer Surfaces and Interfaces. Wiley, New York, p 201Google Scholar
  10. 10.
    Brash J, Horbett TA (eds) (1987) Proteins at Interfaces, Physicochemical and Biochemical Studies. ACS Symposium Series 343, ACS, Washington, DCGoogle Scholar
  11. 11.
    Haynes Ch, Norde W (1995) J Colloid Interface Sci 169:313Google Scholar
  12. 12.
    Matsuda (Andrade, Hlady Adv Polym Sci)Google Scholar
  13. 13.
    Blomberg E, Claesson PM, Fröberg JC, Tilton RD (1994) Langmuir 10:2325Google Scholar
  14. 14.
    Hlady V, personal communicationGoogle Scholar
  15. 15.
    Cullen DC, McKerr G, Hughes EM (1993) Europ Microscopy Anal 25:29Google Scholar
  16. 16.
    Cricenti A, Scarselli M, Generosi R, Selci S, Djaczenko W, Chiarotti G (1992) ACS Polym Prep 33(1):741Google Scholar
  17. 17.
    Bottomley LA, Haseltine JN, Allison DP, Warmack RJ, Thundat T, Sachleben RA, Brown GM, Woychik RP, Jacobson KB, Ferrell TL (1992) J Vac Sci Technol A 10:591Google Scholar
  18. 18.
    Rocca-Serra J, Thimonier J, Chauvin J-P, Barbet J (1994) J Vac Sci Technol B 12:1490Google Scholar
  19. 19.
    Cricenti A, Scarselli MA, Paleari R, Mosca A (1994) J Vac Sci Technol B 12:1494Google Scholar
  20. 20.
    Chernoff EAG, Chernoff D (1992) J Vac Sci Technol A 10:596Google Scholar
  21. 21.
    Takeyasu K, Paul JK, Nettikadan SR, Wang S, Ishii T, Yu H, Yamaguchi M (1994) Europ Microscop Anal 32:7Google Scholar
  22. 22.
    Lin JN, Drake B, Lea AS, Hansma PK, Andrade JD (1990) Langmuir 6:509Google Scholar
  23. 23.
    Andrade JD, Hlady V, Wei A-P, Ho C-H, Lea AS, Jeon SI, Lin YS, Stroup E (1992) Clinical Mater 11:67Google Scholar
  24. 24.
    Lea AS, Pungor A, Hlady V, Andrade JD, Herron JS, Voss EW Jr (1992) Langmuir 8:68Google Scholar
  25. 25.
    Perutz M (1992) Protein Structure New Approaches to Disease and Therapy, WH Freeman Co, New York, p 252Google Scholar
  26. 26.
    Carter DC, He X, Munson SH, Twigg PD, Gernert KM, Broom MB, Miller TY (1989) Science 244:1195Google Scholar
  27. 27.
    Carter DC, He X (1990) Science 249:302Google Scholar
  28. 28.
    He NX, Carter DC (1992) Nature 358:209Google Scholar
  29. 29.
    Andrade JD, Hlady V, Wei A-P, Gölander C-G (1990) Croat Chim Acta 63:527Google Scholar
  30. 30.
    Blomberg E, Claeson PM, Tilton RD (1994) J Colloid Interface Sci 166:427Google Scholar
  31. 31.
    Colvin M, Smolka A, Chang M (1988) In: Rembaum A, Tökés ZA (eds) Microspheres: Medical and Biological Applications. CRC Press, Boca Raton, F1, p 1Google Scholar
  32. 32.
    Margel S (1984) J Polym Sci, Polym Chem Ed 22:3251Google Scholar
  33. 33.
    Basinska T, Slomkowski S (1993) In: Guilbault GG, Mascini M, Uses of Immobilized Biological Compounds, NATO ASI Series 252, Kluwer Academic Publishers, Dordrecht, p. 453Google Scholar
  34. 34.
    Miksa B, Slomkowski S, J Biomater Sci, Polym Ed, in pressGoogle Scholar
  35. 35.
    Basinska T, Slomkowski S, Delamar M (1993) J Bioact Compact Polym 8:205Google Scholar
  36. 36.
    Slomkowski S, Kowalczyk D, Trznadel M, Kryszewski M (1994) ACS Polym Prep 35(2):409Google Scholar
  37. 37.
    Weetal HH (1970) Biochim Biophys Acta 212:1Google Scholar
  38. 38.
    Uzgiris EE, Fromageot HPM (1976) Biopolymers 15:257Google Scholar

Copyright information

© Steinkopff Verlag 1996

Authors and Affiliations

  • D. Kowalczyk
    • 1
  • J. -P. Marsault
    • 2
  • S. Slomkowski
    • 1
  1. 1.Center of Molecular and Macromolecular StudiesPolish Academy of SciencesLodzPoland
  2. 2.Denis Diderto Institute de Topologie et de Dynamique des SystèmesUniversité Paris 7ParisFrance

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