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Colloid and Polymer Science

, Volume 272, Issue 1, pp 72–81 | Cite as

Synthesis and characterization of cationic latex particles bearing sulfhydryl groups and their use in the immobilization of Fab antibody fragments (1)

  • T. Delair
  • C. Pichot
  • B. Mandrand
Original Contributions

Abstract

Emulsion copolymerization of styrene and a cationic monomer vinylbenzyl-isothiouronium chloride (VBIC) with initiation by 2,2′ azobis(2-amidinopropane)-dihydrochloride (V-50) gave monodisperse latex particles. After post-stabilization with cationic or non-ionic surfactants, the colloids were diluted in basic buffers with concomitant deprotection of the sulfhydryl groups. Therefore, coupling reactions on the latex beads were possible. Coupling conditions were determined with Ellman's reagent.14C iodoacetamide and anti C-reactive protein antibody Fab fragments were immobilized and finally, the immunological activity of the sensitized latex was assessed.

Key words

Emulsion polymerization functionalized particles sulfhydryl groups antibody fragments immobilization 

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References

  1. 1.
    French patent 91.06068 (1991), inv. Th. DelairGoogle Scholar
  2. 2.a)
    Bangs LB (1985) “Uniform Latex Particles”, Technical notes Seradyn;Google Scholar
  3. 2.b)
    Rhône-Poulene Estapor “Diagnostic” booklet, and references thereinGoogle Scholar
  4. 3.
    Yamaguchi K, Watanabe S, Nakahama S (1989) Makromol Chem, Rapid Commun 10:397Google Scholar
  5. 4.
    U.S. patent 3 206 748 (1966), inv. S.J. Nelson Jr.; Chem, Abstr 65:10528c (1966)Google Scholar
  6. 5.
    Ellmann SJ (1959) Arch Biochem Biophys 82:70Google Scholar
  7. 6.
    Bradford M (1976) Anal Biochem 72:248Google Scholar
  8. 7.
    French Patent 90 08825 (1990) inv. D. Darbouret, Charles MH, Theretz A, Mandrand BGoogle Scholar
  9. 8.a)
    Goodwin JW, Ottewill RH, Pelton R (1979) Colloid and Polymer Sci 257:61;Google Scholar
  10. 8.b)
    Guthrie WH, PhD Dissertation, 1985, Lehigh University.Google Scholar
  11. 9.a)
    Juang MS, Krieger IM (1976) J Polym Sci, Polym Chem Edn 14:2089;Google Scholar
  12. 9.b)
    Liu LJ, Krieger IM (1981) J Polym Sci, Polym Chem Edn 19:3013;Google Scholar
  13. 9.c)
    Brouwer WM, Van Der Vegt M, Van Haeren P (1990) Eur Polym J 26:35;Google Scholar
  14. 9.d)
    Kim JH, Chainey M, El-Aasser M, Vanderhoff JW (1989) J Polym Sci A27:3187;Google Scholar
  15. 9.e)
    Ohtsuka Y, Kawaguchi H, Hagashi S (1981) Polym. J. 22:658;Google Scholar
  16. 9.f)
    Van Streun KH, Belt WJ, Piet P, German AL (1991) Eur Polym J 27:931;Google Scholar
  17. 9.g)
    Twigt F, Piet P, German AL (1991) Eur Polym J 27:939Google Scholar
  18. 10.
    Stoll S, PhD Dissertation, 1992, Université de StrasbourgGoogle Scholar
  19. 11.
    Kronberg B, Käll L, Stenius P (1981) J Dispersion Science and Technology 2:215Google Scholar
  20. 12.
    Pelton R, “Chemical reactions at the latex-solution interface”, in “Scientific Methods for the Study of Polymer Colloids and Their Applications”, Candau F, Ottewill RH (eds.) Kluwer Academic Publishers, 1990, p. 493Google Scholar

Copyright information

© Steinkopff-Verlag 1994

Authors and Affiliations

  • T. Delair
    • 1
  • C. Pichot
    • 1
  • B. Mandrand
    • 1
  1. 1.Unité Mixte BioMérieux-CNRSLyon CedexFrance

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