Abstract
Apparent molar volumes and viscosity B- and D- coefficients of amino acids glycine, valine, proline, serine and arginine have been determined in water and in aqueous 6M guanidine hydrochloride (GuHCl) solution at 25°C. Transfer volumes and transfer viscosity B-coefficients were evaluated for the amino acids studied in going from water to 6M GuHCl. These transfer properties which were all positive were interpreted in terms of strong interactions of GuHCl molecules with the charged centers of amino acid molecules. A comparison of results obtained in this work for GuHCl and those obtained from literature for urea has shown that GuHCl has stronger interactions than urea with amino acids. This finding explained the previous experimental observations on GuHCl being a stronger denaturing agent than urea for proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. Joly,A Physicochemical Approach to the Denaturation of Proteins (Academic press, London, 1965), pp 28.
C. Jolicoeur, B. Riedl, D. Desrochers, L. L. Lemelin, R. Zamojka, and O. Enea,J. Solution Chem. 15, 109 (1986).
M. Abu-Hamdlyyah and A. Shehabuddin,J. Chem. Eng. Data 27, 74 (1982).
O. Enea and C. Jolicoeur,J. Phys. Chem. 86, 3870 (1982).
B. E. Conway,J. Solution Chem. 7, 721 (1978).
E. Ayranci and B. E. Conway,J. Chem. Soc. Faraday Trans. I 79, 1357 (1983).
B. E. Conway and E. Ayranci,J. Chem. Thermodyn. 20, 9 (1988).
Y. Nozaki,Methods Enzymology 26, 43 (1972).
B. E. Conway, R. E. Verral, and J. E. Desnoyers,Trans. Faraday Soc. 62, 2738 (1966).
J. E. Desnoyers and M. Arel,Can. J. Chem. 45, 359 (1967).
G. S. Kell,J. Chem. Eng. Data 12, 66 (1967).
F. J. Millero, inStructure and Transport Process in Water and Aqueous Solutions, R. A. Horne, ed., (Wiley, New York, 1972), Chap. 12.
G. Jones and M. Dole,J. Am. Chem. Soc. 51, 2950 (1929).
F. J. Millero, A. Losurdo, and C. Shin,J. Phys. Chem. 82, 784 (1978).
L. S. Mason, P. M. Kampmeyer, and A. L. Robinson,J. Am. Chem. Soc. 74, 1287 (1952).
G. Kumar, N. C. Dey, and B. K. Saikia,Z. Phys. Chem. Neue Folge 149, 183 (1986).
Y. Nozaki and C. Tanford,J. Biological Chem. 7, (1970).
V. Prakash, C. Loucheux, S. Scheufele, M. J. Gorbunoff, and S. N. Timasheff,Archives of Biochem. Biophys. 210, 455 (1981).
F. J. Castelino and R. Baker,Biochemistry 7, 4135 (1968).
B. R. Saresh Chandra, A. G. Appo Rao, and M. S. Narasinga Rao,J. Agric. Food Chem. 32, 1402 (1984).
E. Ayranci and A. Kaya,Doga Turkish J. Chem. (in press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Belibaĝli, K.B., Ayranci, E. Viscosities and apparent molar volumes of some amino acids in water and in 6M guanidine hydrochloride at 25°C. J Solution Chem 19, 867–882 (1990). https://doi.org/10.1007/BF00653072
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00653072