Abstract
The effects of urea, guanidinium chloride, and potassium chloride on the solution properties of caffeine have been investigated by solubility and partition experiments and by measuring heats of dilution, solution, and mixing. Thermodynamic transfer properties are used to discuss the possible mechanism of action of the two denaturants. The ability of caffeine to form stacked aggregates is taken as possible evidence for a direct interaction of this solute with urea and guanidinium ion. The data are discussed both in terms of an ideally associating system and in terms of the virial expansion following the McMillan-Mayer solution theory.
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Cesàro, A., Russo, E. & Tessarotto, D. Thermodynamics of caffeine in aqueous denaturant solutions. J Solution Chem 9, 221–235 (1980). https://doi.org/10.1007/BF00648328
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DOI: https://doi.org/10.1007/BF00648328