Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 324, Issue 1, pp 15–19 | Cite as

Inhibition by α2-adrenoceptor agonists of the secretion of catecholamines from isolated adrenal medullary cells

  • S. Sakurai
  • A. Wada
  • F. Izumi
  • H. Kobayashi
  • N. Yanagihara
Article

Summary

In adrenal medullary cells, carbachol evokes the secretion of catecholamines with simultaneous uptake of45Ca. Highly selective agonists for α2-adrenoceptors, clonidine, naphazoline, guanfacine, tramazoline and oxymetazoline inhibited carbachol evoked secretion of catecholamines in a dose-dependent manner. These α2-agonists also inhibited the uptake of45Ca evoked by carbachol with similar dose-response curve to inhibition of catecholamine secretion. On the contrary, highly selective agonists for α1-adrenoceptors, phenylephrine and norfenefrine did not inhibit the secretion of catecholamines and cellular uptake of45Ca. The inhibition by α2-agonists was not restored either by the increase in carbachol, or Ca concentrations, suggesting that the mode of inhibition was distinct from competition at cholinergic receptors or Ca-channels. It is likely that α2-agonists inhibited the secretion of catecholamines via the inhibition of Ca uptake which was probably caused through the activation of α2-adrenoceptors.

Key words

Adrenal medulla Catecholamine Secretion Calcium ion α2-Agonists α2-Adrenoceptor 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. Sakurai
    • 1
  • A. Wada
    • 1
  • F. Izumi
    • 1
  • H. Kobayashi
    • 1
  • N. Yanagihara
    • 1
  1. 1.Department of PharmacologyUniversity of Occupational and Environmental Health, School of MedicineFukuokaJapan

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