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Inhibition by α2-adrenoceptor agonists of the secretion of catecholamines from isolated adrenal medullary cells

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Summary

In adrenal medullary cells, carbachol evokes the secretion of catecholamines with simultaneous uptake of45Ca. Highly selective agonists for α2-adrenoceptors, clonidine, naphazoline, guanfacine, tramazoline and oxymetazoline inhibited carbachol evoked secretion of catecholamines in a dose-dependent manner. These α2-agonists also inhibited the uptake of45Ca evoked by carbachol with similar dose-response curve to inhibition of catecholamine secretion. On the contrary, highly selective agonists for α1-adrenoceptors, phenylephrine and norfenefrine did not inhibit the secretion of catecholamines and cellular uptake of45Ca. The inhibition by α2-agonists was not restored either by the increase in carbachol, or Ca concentrations, suggesting that the mode of inhibition was distinct from competition at cholinergic receptors or Ca-channels. It is likely that α2-agonists inhibited the secretion of catecholamines via the inhibition of Ca uptake which was probably caused through the activation of α2-adrenoceptors.

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Authors and Affiliations

  1. Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1, Iseigaoka, Kitakyushu, 807, Fukuoka, Japan

    S. Sakurai, A. Wada, F. Izumi, H. Kobayashi & N. Yanagihara

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  1. S. Sakurai
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  2. A. Wada
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  3. F. Izumi
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  4. H. Kobayashi
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  5. N. Yanagihara
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Sakurai, S., Wada, A., Izumi, F. et al. Inhibition by α2-adrenoceptor agonists of the secretion of catecholamines from isolated adrenal medullary cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 324, 15–19 (1983). https://doi.org/10.1007/BF00647832

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  • DOI: https://doi.org/10.1007/BF00647832

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