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Histochemistry

, Volume 63, Issue 2, pp 129–143 | Cite as

Protein content, dry mass and chemical composition of individual mast cells related to body growth

  • L. Mellblom
  • L. Enerbäck
Article

Summary

Recently developed quantitative microscopical techniques were used to study relations between body growth and protein content as well as dry mass of individual mast cells. Since previous studies had shown an age-related increase of mast cell content of 5-hydroxytryptamine (5-HT) and heparin, these mast cell components were also included in the present study. The cells were obtained from the peritoneal cavity of rats aged 44–269 days (body weights 189–610 g). All studied mast cell parameters showed an increase that was related to the growth of the animals. The dry mass increased 60%, protein 50%, heparin 50% but 5-HT increased as much as 260% during the studied growth period. There was a mutual and linear correlation between all studied mast cell parameters. Population studies, based on large scale measurements of individual mast cells from young and adult rats, were made. These studies showed that histograms of 5-HT content, protein content and dry mass of individual mast cells were skewed with a tail towards higher values and approximately lognormal. On the other hand, the frequency distribution of heparin content of individual mast cells was approximately normal.

Keywords

Heparin Protein Content Mast Cell Frequency Distribution Linear Correlation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aitchison, J., Brown, J.A.C.: The log normal distribution. Cambridge: University Press 1963Google Scholar
  2. Allen, A.M.: Mitosis and binucleation in mast cells of the rat. J. Natl. Cancer Inst.28, 1125–1143 (1962)Google Scholar
  3. Benditt, E.P., Arase, M., Roeper, M.E.: Histamine and heparin in isolated rat mast cells. J. Histochem. Cytochem.4, 419–420 (1956)Google Scholar
  4. Bergendorff, A., Uvnäs, B.: Storage of 5-hydroxytryptamine in rat mast cells. Evidence for an ionic binding to carboxyl groups in a granule heparin-protein complex. Acta Physiol. Scand.84, 320–331 (1972)Google Scholar
  5. Berlin, G., Enerbäck, L.: Changes in numbers and heparin content of peritoneal fluid mast cells of growing rats measured by flow cytofluorometry. J. Histochem. Cytochem.26, 14–21 (1978)Google Scholar
  6. Blenkinsopp, W.K.: Mast cell proliferation in adult rats. J. Cell Sci.2, 33–37 (1967)Google Scholar
  7. Burton, A.: Histochemical studies on developing mast cells. Anat. Rec.150, 265–270 (1964)Google Scholar
  8. Carlsson, L.: Cytochemical determination of dry mass. Thesis, Karolinska Institutet, Stockholm, Sweden (1970)Google Scholar
  9. Carlsson, S.-A., Ritzén, M.: Mast cells and 5-HT. Intracellular release of 5-hydroxytryptamine (5-HT) from storage granules during anaphylaxis or treatment with compound 48/80. Acta Physiol. Scand.77, 449–464 (1969)Google Scholar
  10. Combs, J.W., Lagunoff, D., Benditt, E.P.: Differentiation and proliferation of embryonic mast cells of the rat. J. Cell Biol.25, 577–592 (1965)Google Scholar
  11. Corrodi, H., Hillarp, N.-Å., Jonsson, G.: Fluorescence methods for the histochemical demonstration of monoamines. 3. Sodium borohydride reduction of the fluorescent compounds as a specificity test. J. Histochem. Cytochem.12, 582–586 (1964)Google Scholar
  12. Deierkauf, F.A., Heslinga, F.J.M.: The action of formaldehyde on rat brain lipids. J. Histochem. Cytochem.10, 79–82 (1962)Google Scholar
  13. Diamant, B., Lowry, O.H.: Dry weight determination of single lyophilized mast cells of the rat. J. Histochem. Cytochem.14, 519–524 (1966)Google Scholar
  14. Diamant, B., Glick, D.: Certain dehydrogenase activities and dry weight of normal and malignant mast cells. J. Histochem. Cytochem.15, 695–701 (1967)Google Scholar
  15. Diamant, B., von Redlich, D., Glick, D.: Comparison of the phenol-reagent and bromsulfalein-binding methods for determination of protein and its measurement in mast cells. Anal. Biochem.21, 135–146 (1967)Google Scholar
  16. Enerbäck, L.: Berberine sulphate binding to mast cell polyanions: a cytofluorometric method for the quantitation of heparin. Histochemistry42, 301–313 (1974)Google Scholar
  17. Enerbäck, L.: Quantitative studies of 5-hydroxytryptamine, heparin and DNA of mast cells in relation to body growth. J. Cell Biol.70, 269a (1976)Google Scholar
  18. Enerbäck, L., Häggendal, J.: Uptake of dopamine by rat mast cells in vivo. Pathol. Eur.5, 401–408 (1970)Google Scholar
  19. Enerbäck, L., Johansson, K.-A.: Fluorescence fading in quantitative fluorescence microscopy: a cytofluorometer for the automatic recording of fluorescence peaks of very short duration. Histochem. J.5, 351–362 (1973)Google Scholar
  20. Enerbäck, L., Jarlstedt, J.: A cytofluorometric and radiochemical analysis of the uptake and turnover of 5-hydroxytryptamine in mast cells. J. Histochem. Cytochem.23, 128–135 (1975)Google Scholar
  21. Enerbäck, L., Franzén, L., Norrby, K.: A tissue model for the study of cell proliferation parameters in vivo. Histochemistry47, 207–218 (1976)Google Scholar
  22. Enerbäck, L., Gustafsson, B., Mellblom, L.: Cytofluorometric quantitation of 5-hydroxytryptamine in mast cells: an improved technique for the formaldehyde condensation reaction. J. Histochem. Cytochem.25, 32–41 (1977)Google Scholar
  23. Enerbäck, L., Mellblom, L.: 5-hydroxytryptamine content of mast cells, mast cell numbers and body growth. Exp. Cell Res.112, 103–109 (1978a)Google Scholar
  24. Enerbäck, L., Mellblom, L.: Growth related changes in the content of heparin and 5-hydroxytryptamine of mast cells. Cell Tissue Res.187, 367–378 (1978b)Google Scholar
  25. Enerbäck, L., Svensson, I.: A method for the isolation of peritoneal mast cells with high yield and purity. (1979, in preparation)Google Scholar
  26. Enerbäck, L., Johansson, U.: Cytofluorometric determination of total protein in individual cells with an o-phthalaldehyde reagent. (1979, in preparation)Google Scholar
  27. Enerbäck, L., Mellblom, L.: Cytofluorometric determination of total protein in mast cells. (1979, in preparation)Google Scholar
  28. Enerbäck, L., Wingren, U.: Histamine content of peritoneal and tissue mast cells of growing rats. (1979, in preparation)Google Scholar
  29. Enesco, M., Leblond, C.P.: Increase in cell numbers as a factor in the growth of the organs and tissues of the young male rat. J. Embryol. Exp. Morphol.10, 530–562 (1962)Google Scholar
  30. Fillion, G.M.B., Slorach, S.A., Uvnäs, B.: The release of histamine, heparin and granule protein from rat mast cells treated with compound 48/80 in vitro. Acta Physiol. Scand.78, 547–560 (1970)Google Scholar
  31. Glick, D., Hosoda, S.: Histochemistry. LXXVIII. Ascorbic acid in normal mast cells and macrophages and in neoplastic mast cells. Proc. Soc. Exp. Biol. Med.119, 52–56 (1965)Google Scholar
  32. Glick, D., von Redlich, D., Diamant, B.: Heparin, histamine, and serotonin in normal and malignant mast cells, and nanogram determination of serotonin. Biochem. Pharmacol.16, 553–559 (1967)Google Scholar
  33. Gottfried, E.L.: Lipid patterns of leucocytes in health and disease. Semin. Hematol.9, 241–250 (1972)Google Scholar
  34. Guidotti, G.: Turnover of the sulphate groups in mast cells of young and adult rats. Exp. Cell Res.12, 659–661 (1957)Google Scholar
  35. Guidotti, G.G., Spinelli-Ressi, F.: Incorporation of sulphate in peritoneal mast cells isolated from young and adult rats. Exp. Cell Res.33, 254–263 (1964)Google Scholar
  36. Gustafsson, B., Enerbäck, L.: Cytofluorometric quantitation of 5-hydroxytryptamine and heparin in individual mast cell granules. J. Histochem. Cytochem.26, 47–54 (1978)Google Scholar
  37. Hoffman, J.G.: The size and growth of tissue cells. Springfield: Charles C. Thomas 1953Google Scholar
  38. Kapteyn, J.C.: Skew frequency curves in biology and statistics. Rec. Trav. Bot. Neerl.13, 105–157 (1916)Google Scholar
  39. Lagunoff, D., Phillips, M.T., Iseri, O.A., Benditt, E.P.: Isolation and preliminary characterization of rat mast cell granules. Lab. Invest.13, 1331–1344 (1964)Google Scholar
  40. Meyer, S.L., Saunders, A.M.: Cytofluorometric study of mast cell polyanions II. Adult rat peritoneal mast cells regenerating after polymyxin treatment. J. Histochem. Cytochem.17, 56–61 (1969)Google Scholar
  41. Olsson, Y., Sjöstrand, J.: Proliferation of mast cells in peripheral nerves during Wallerian degeneration. Acta Neuropathol.13, 111–121 (1969)Google Scholar
  42. Ottoson, R., Kahn, K., Glick, D.: Studies in histochemistry. XLVIII. Dry mass of mast cells measured by interference microscopy and X-ray absorption. Exp. Cell Res.14, 567–574 (1958)Google Scholar
  43. Padawer, J., Gordon, A.S.: Peritoneal fluid mast cells: their numbers and morphology in rats of various body weights (ages). J. Gerontol.11, 268–272 (1956)Google Scholar
  44. Padawer, J.: Quantitative studies with mast cells. Ann. NY Acad. Sci.103, 87–137 (1963)Google Scholar
  45. Padawer, J.: Mast cells: extended lifespan and lack of granule turnover under normal in vivo conditions. Exp. Mol. Pathol.20, 269–280 (1974)Google Scholar
  46. Parekh, A.C., Glick, D.: Studies in histochemistry. LXV. Heparin and hexosamine in isolated mast cells: determination, intracellular distribution, and effects of biological state. J. Biol. Chem.237, 280–286 (1962)Google Scholar
  47. Radden, B.G.: Cytochemical variations in the granules of rat mast cells, and their relation to mast cell maturation. Austral. J. Exp. Biol Med. Sci40, 9–16 (1962)Google Scholar
  48. Ritzén, M.: Mast cells and 5-HT. Uptake of labelled 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan in relation to storage of 5-HT in individual rat mast cells. Acta Physiol. Scand.70, 42–53 (1967)Google Scholar
  49. Roth, E., Noltenius, H., Oehlert, W.: Autoradiographische Untersuchungen zur DNS-Neubildung und zum Sulfateinbau in Gewebsmastzellen. Frankfurt. Z. Pathol.73, 40–51 (1963)Google Scholar
  50. Rundquist, I., Enerbäck, L.: Millisecond fading and recovery phenomena in fluorescent biological objects. Histochemistry47, 79–87 (1976)Google Scholar
  51. Slorach, S.A.: Histamine and heparin release from isolated rat mast cells exposed to compound 48/80. Acta Physiol. Scand.82, 91–97 (1971)Google Scholar
  52. Tas, J.: The alcian blue and combined alcian blue-safranin O staining of glycosaminoglycans studied in a model system and in mast cells. Histochem. J.9, 205–230 (1977)Google Scholar
  53. Thomson, R.Y., Heagy, F.C., Hutchison, W.C., Davidson, J.N.: The deoxyribonucleic acid content of the rat cell nucleus and its use in expressing the results of tissue analysis, with particular reference to the composition of liver tissue. Biochem. J.53, 460–474 (1953)Google Scholar
  54. Walker, B.E.: Mast cell turn-over in adult mice. Nature192, 980–981 (1961)Google Scholar
  55. Wilks, S.S.: Mathematical statistics. New York: Wiley and Sons 1962Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • L. Mellblom
    • 1
  • L. Enerbäck
    • 1
  1. 1.Department of Pathology IIUniversity of LinköpingLinköpingSweden

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