Molecular Biology Reports

, Volume 3, Issue 3, pp 243–249 | Cite as

Studies of the UV-sensitivity of virus-specific RNA synthesis in cells infected or transformed by adenovirus 5 and SV 40

  • V. L. Mantieva
  • P. M. Chumakov
  • Yu. V. Kozlov
  • A. A. Shelov
  • E. S. Zalmanzon
  • A. A. Savina


The effect of UV-irradiation on host and virus-specific RNA synthesis in cells infected or transformed by tumor viruses (Ad 5 and SV 40) was studied.

It was found that the synthesis of host and Ad 5 RNA in infected KB cells was almost equally inhibited upon UV-irradiation; the transcription of the EcoRI produced fragments-fragment B was inhibited to a greater extent than fragment A, suggesting that the transcription of the whole Ad 5 genome starts from the left side. The transcription of viral sequences in transformed cells, on the other hand, was more resistant to UV-irradiation than the transcription of the host ones. The results obtained suggest that the small size of the virus transcriptions, or their location at the beginning of large host transcriptions, may be responsible for the observed data.


Viral Sequence Virus Transcription Large Host Host Transcription 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© D. Reidel Publishing Company 1977

Authors and Affiliations

  • V. L. Mantieva
    • 1
  • P. M. Chumakov
    • 1
  • Yu. V. Kozlov
    • 1
  • A. A. Shelov
    • 1
  • E. S. Zalmanzon
    • 1
  • A. A. Savina
    • 1
  1. 1.Institute of Molecular BiologyAcademy of Sciences of the U.S.S.R.MoscowU.S.S.R.

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