Skip to main content
SpringerLink
Log in

Polyacrylamide gel as a medium for DNA dissociation and reassociation

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Several properties of thermal denaturation and renaturation of DNA in polyacrylamide gels were investigated: (1) Following electrophoresis the DNA band was scanned and shown to increase in absorbance with increasing temperature. The increase was proportioned to DNA concentration across the peak. (2) The dependence of theT m on salt concentration over a hundred fold range was similar to that found for DNA in free solution. (3) Denaturation of several DNA samples ranging in G+C content from 26 to 71% was compared in gels and free solution. The relationship betweenT m and % G+C was virtually identical for both sets of DNAs. (4) The kinetics of DNA renaturation in the gel was followed. Reassociation of bacteriophageT 4 DNA was 2nd order and proceeded more rapidly in polyacrylamide gels than in free solution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rosner, A., Edelman, M., and Gressel, J.,Anal. Biochem. 58, 602 (1974).

    Google Scholar 

  2. Edelman, M., Cowan, C.A., Epstein, H.T., and Schiff, J.A.,Proc. Nat. Acad. Sci. U.S.A. 52, 1214 (1964).

    Google Scholar 

  3. Kislev, N., Edelman, M., and Harpaz, I.,J. Invert. Pathol. 17, 199 (1971).

    Google Scholar 

  4. Kadouri, A., Atsmon, D., and Edelman, M.,Proc. Nat. Acad. Sci. U.S.A. 72, 2260 (1975).

    Google Scholar 

  5. Gressel, J., Rosner, A., and Cohen, N.,Anal. Biochem. 69, 84 (1975).

    Google Scholar 

  6. Wetmur, J. and Davidson, N.,J. Mol. Biol. 31, 349 (1968).

    Google Scholar 

  7. Marmur, J. and Doty, P.,J. Mol. Biol. 5, 109 (1962).

    Google Scholar 

  8. Schildkraut, C.L., Marmur, J., and Doty, P.,J. Mol. Biol. 4, 430 (1962).

    Google Scholar 

  9. Britten, R.J., Graham, E.D., and Neufeld, B.R.: in L. Grossman and K. Moldave (eds.),Methods in Enzymology, Academic Press, New York and London, 1974, Vol. XXIX, p. 363.

    Google Scholar 

Download references

Author information

Author notes

  1. Arie Rosner

    Present address: Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam-Buitenveldert, The Netherlands

Authors and Affiliations

  1. Dept. of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel

    Arie Rosner, Dina Heller & Marvin Edelman

Authors
  1. Arie Rosner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dina Heller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marvin Edelman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rosner, A., Heller, D. & Edelman, M. Polyacrylamide gel as a medium for DNA dissociation and reassociation. Mol Biol Rep 3, 207–212 (1977). https://doi.org/10.1007/BF00643474

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00643474

Keywords

Search

Navigation