Molecular Biology Reports

, Volume 3, Issue 3, pp 203–206 | Cite as

The substance reacting with SRBC (sheep red blood cells) and rabbit IgG. Isolation under mild conditions from rat thymus

  • I. A. Bezvershenko
  • M. G. Boyko
  • L. M. Bykova


A factor reacting with SRBC and rabbit IgG was obtained under mild conditions from rat thymus and spleen.

The isolation procedure includes incubation of thymocytes or splenocytes with IgG-cellulose adsorbent, destruction of cells, washing the adsorbent and elution of an adsorbed material at pH 2. This preparation as well as the purified substance previously obtained by affinity chromatography on IgG-cellulose columns were found to agglutinate both SRBC and autologous erythrocytes. Preincubation in 1% SDS leads to dissociation of the preparation into several components separated by gel electrophoresis.

A probable relation of this structure to the rosette forming capacity of T-lymphocytes is discussed.


Blood Cell Affinity Chromatography Mild Condition Probable Relation Isolation Procedure 
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  1. 1.
    Bezvershenko, I.A., Bykova, L.M., and Lukashova, R.G.,Ukrainian Biochemical J. 47, 85 (1975).Google Scholar
  2. 2.
    Bezvershenko, I.A.,FEBS Letters 61, 91 (1976).Google Scholar
  3. 3.
    Katz, D.M. and Benacerraf, B.,Advances in Immunol. 15, 94 (1972).Google Scholar
  4. 4.
    March, S.C., Parikh, I. and Cuatrecasas, P.,Analyt. Biochem. 60, 149 (1974).Google Scholar
  5. 5.
    Lowry, H.O., Rosebroug, T.M., Farr, G.A., and Randall, R.I.,J. Biol. Chem. 193, 265 (1951).Google Scholar
  6. 6.
    Davis, B.J.,Ann. N.Y. Acad. Sci. 121, 404 (1964).Google Scholar
  7. 7.
    Fairbanks, G., Steck, T.L., and Wallach, D.F.H.,Biochemistry 10, 2606 (1971).Google Scholar
  8. 8.
    Wekerle, M., Cohen, I.R., and Feldman, M.,Nature New Biol. 271, 25 (1973).Google Scholar
  9. 9.
    Wekerle, M., Cohen, I.R., and Feldman, M.,Deutsche Med. Wochenschr. 99, 1734 (1974).Google Scholar
  10. 10.
    Gluckman, I.C., and Montambault, P.,Clin. Exp. Immunol. 22, 302 (1975).Google Scholar
  11. 11.
    Gluckman, I.C., and Legran, M.,Transplant. Proc. 7 suppl. 1, 407 (1975).Google Scholar
  12. 12.
    Wortis, H.H., Cooper, A.G., and Brown, M.C.,Nature New Biol. 243, 109 (1973).Google Scholar
  13. 13.
    Lay, W.H., Mendes, N.F., Bianco, C., and Nussenzweig, V.,Nature (Lond.)230, 531 (1971).Google Scholar
  14. 14.
    Sandilands, G., Gray, K., Cooney, A., Browning, I.D., and Anderson, I.R.,Lancet i, 27 (1974).Google Scholar

Copyright information

© D. Reidel Publishing Company 1977

Authors and Affiliations

  • I. A. Bezvershenko
    • 1
  • M. G. Boyko
    • 1
  • L. M. Bykova
    • 1
  1. 1.Institute of Endocrinology and MetabolismMinistry of Public HealthKievUkrainian S.S.R.

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