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The majority of T lymphocytes are polyclonal during the chronic phase of chronic myelogenous leukemia

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Abstract

To clarify the extent of cell lineage involvement in chronic myelogenous leukemia (CML), we investigated the bcr gene rearrangement and clonality using the X-chromosome-linked restriction fragment length polymorphism (RFLP) methylation method in T lymphocytes and granulocytes. We examined the granulocyte and T-cell fractions from the peripheral blood of seven female patients with CML during the chronic phase; patients were heterozygous for RFLPs at the phosphoglycerate kinase (PGK) or the hypoxanthine phosphoribosyltransferase (HPRT) gene. RFLP-methylation analysis of granulocytes demonstrated a monoclonal pattern in six of the seven patients and a rearrangedbcr gene in all seven patients. In contrast, T lymphocytes exhibited a polyclonal pattern in six cases; in one case, a faint band was observed following methyl-sensitive enzyme cleavage. Thebcr gene analysis in T lymphocytes showed the germline in every case. Our results indicate that the majority of T lymphocytes are polyclonal during the chronic phase of CML and confirm previous reports based on glucose-6-phosphate dehydrogenase, cytogenetic, andbcr rearrangement analyses.

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Authors and Affiliations

  1. Third Department of Internal Medicine, Gunma University School of Medicine, 371, Maebashi, Gunma, Japan

    N. Tsukamoto, M. Karasawa, T. Maehara, K. Okamoto, H. Sakai & T. Naruse

  2. College of Medical Care and Technology, Gunma University School of Medicine, Maebashi, Gunma, Japan

    K. Morita & J. Tsuchiya

  3. Department of Internal Medicine, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, Japan

    M. Omine

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  1. N. Tsukamoto
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  2. M. Karasawa
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  3. T. Maehara
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  4. K. Okamoto
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  5. H. Sakai
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  6. T. Naruse
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  7. K. Morita
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  8. J. Tsuchiya
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  9. M. Omine
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Tsukamoto, N., Karasawa, M., Maehara, T. et al. The majority of T lymphocytes are polyclonal during the chronic phase of chronic myelogenous leukemia. Ann Hematol 72, 61–65 (1996). https://doi.org/10.1007/BF00641309

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  • DOI: https://doi.org/10.1007/BF00641309

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