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Histochemistry

, Volume 75, Issue 4, pp 473–484 | Cite as

Vascular and tubular renin in the kidneys of mice

  • R. Taugner
  • E. Hackenthal
  • T. Inagami
  • R. Nobiling
  • K. Poulsen
Article
  • 26 Downloads

Summary

Monospecific antisera and the immunocytochemical PAP-method have been used to localize renin in the kidneys of mice. With this procedure, reaction product was not only observed in the epitheloid cells of kidney vessels but also in kidney tubules: in the apical portion of proximal tubule cells and in some cells of the connecting and the cortical collecting tubule. To answer the question, whether the occurrence of renin in kidney tubule cells is the consequence of tubular synthesis or that of glomerular filtration of plasma renin followed by its uptake from the tubular lumen, tracer experiments with radioiodinated renin and with horseradish peroxidase were undertaken. The results of these studies as well as other arguments suggest reabsorptive pinocytosis of the filtered hormone as the source of tubular renin.

Keywords

Public Health Renin Glomerular Filtration Horseradish Peroxidase Proximal Tubule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baumann K (1981) Renal transport of proteins. In: Greger R, Lang F, Silbernagl S (eds) Renal transport of organic substances. Springer, Berlin Heidelberg New York, pp 118–133Google Scholar
  2. Boll H-U, Forssmann WG, Taugner R (1975) Studies on the juxtaglomerular apparatus. IV. Freeze-fracturing of membrane surfaces. Cell Tissue Res 161:459–469Google Scholar
  3. Bourdeau JE, Carone FA, Ganote CE (1972) Serum albumin uptake in isolated perfused renal tubules. Quantitative and electron microscope radioautographic studies in three anatomical segments of the rabbit nephron. J Cell Biol 54:382–398Google Scholar
  4. Carone FA, Peterson DR, Oparil S, Pullmann TN (1979) Renal tubular transport and catabolism of proteins and peptides. Kidney Int 16:271–278Google Scholar
  5. Cohen S, Taylor JM, Marakami K, Michelakis AM, Inagami T (1972) Isolation and characterization of renin-like enzymes from mouse submaxillary glands. Biochemistry 11:4286–4293Google Scholar
  6. Davidoff M, Schiebler TH (1981) Immunocytochemical demonstration of renin in the mouse and rat kidney after adrenalectomy. Histochemistry 72:453–457Google Scholar
  7. Forssmann WG, Ito S, Weihe E, Aoki A, Dym M, Fawcett D-W (1977) An improved perfusion fixation method for the testis. Anat Rec 188:307–314Google Scholar
  8. Hackenthal E, Poulsen K, Taugner Ch, Taugner R (1980) Localization of renin in mouse kidney by the peroxidase-antiperoxidase technique. In: Gross F, Vogel G (eds) Enzymatic release of vasoactive peptides. Raven Press, New York, pp 67–72Google Scholar
  9. Hunter WM, Greenwood FC (1962) Preparation of131J-labeled human growth hormone of high specific activity. Nature 194:495–496Google Scholar
  10. Leyssac PP (1978) A micropuncture study of glomerular filtration and tubular reabsorption of endogenous renin in the rat. Renal Physiol (Basel) 1:181–188Google Scholar
  11. Malling C, Poulsen K (1977) A direct radioimmunoassay for plasma renin in mice and its evaluation. Biochim Biophys Acta 491:532–541Google Scholar
  12. Michelakis AM, Yoshida H, Menzie J, Murakami K, Inagami T (1974) A radioimmunoassay for the direct measurement of renin in mice and its application to submaxillary gland and kidney studies. Endocrinology 94:1101–1105Google Scholar
  13. Minuth M, Hackenthal E, Poulsen K, Rix E, Taugner R (1981) Renin immunocytochemistry of the differentiating juxta-glomerular apparatus. Anat Embryol 162:173–181Google Scholar
  14. Rappelli A, Peart WS (1968) Renal excretion of renin in the rat. Circ Res 23:531–537Google Scholar
  15. Rix E, Ganten D, Schüll B, Unger Th, Taugner R (1981) Converting-enzyme in the choroid plexus, brain, and kidney: Immunocytochemical and biochemical studies in rats. Neurosci Lett 22:125–130Google Scholar
  16. Rogers AW (1973) Techniques of autoradiography. Second revised and enlarged edition. Elsevier, AmsterdamGoogle Scholar
  17. Straus W (1964) Occurrence of phagosomes and phago-lysosomes in different segments of the nephron in relation to the reabsorption, transport, digestion, and extrusion of intravenously injected horseradish peroxidase. J Cell Biol 21:295–308Google Scholar
  18. Straus W (1967) Changes in intracellular location of small phagosomes (micropinocytic vesicles) in kidney and liver cells in relation to time after injection and dose of horseradish peroxidase. J Histochem Cytochem 15:381–393Google Scholar
  19. Straus W (1971) Comparative analysis of the concentration of injected horseradih peroxidase in cytoplasmic granules of the kidney cortex, in the blood, urine, and liver. J Cell Biol 48:620–632Google Scholar
  20. Sudo J-i (1981) Distributions of peptidases in the metabolization of peptide hormones particularly angiotensin II, along the isolated single nephron of rat. Folia Pharmacol jpn 78:27–44Google Scholar
  21. Tanaka T, Gresik EW, Michelakis AM, Barka T (1980) Immunocytochemical localization of renin in kidneys and submandibular glands of SWR/J and C57BL/6J mice. J Histochem Cytochem 28:113–118Google Scholar
  22. Taugner Ch, Poulsen K, Hackenthal E, Taugner R (1979) Immunocytochemical localization of renin in mouse kidney. Histochemistry 62:19–27Google Scholar
  23. Taugner R, Wagenmann U (1958) Serienmäßige Herstellung von Gefrierschnitt-Autoradiogrammen mit optimalem Kontakt. Naunyn-Schmiedebergs Arch exp Pathol Pharmakol 234:336–342Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • R. Taugner
    • 1
  • E. Hackenthal
    • 2
  • T. Inagami
    • 2
  • R. Nobiling
    • 1
  • K. Poulsen
    • 3
  1. 1.Institute of Physiology of the University of HeidelbergHeidelbergGermany
  2. 2.Institute of Pharmacology of the University of HeidelbergHeidelbergGermany
  3. 3.Institute of BiochemistryThe Royal Dental CollegeCopenhagenDenmark

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