Simple and inexpensive Flow Injection Analysis for determination of sucrose using invertase and glucose oxidase immobilised on glass beads
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A Flow Injection Analysis (FIA) for sucrose using invertase (E.C. 188.8.131.52), mutarotase (E.C.184.108.40.206) and glucose oxidase (E.C.220.127.116.11) was developed. The enzymes were immobilised on glass beads using glutaraldehyde. The sucrose concentration was related to oxygen saturation. Fall in O2 concentration, as a result of sucrose oxidation, was detected by a low cost, home-made O2 electrode. The system was able to measure sucrose from 0.025 to 100mM with a response time of 6min using 200 μl of sample, with an apparent Km of 42mM of sucrose. The system has been operated satisfactorily for 50 days without loss any initial activity.
KeywordsOxidation Oxygen Glucose Enzyme Sucrose
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- Barlikova, A., Svorc, J. and Miertus, S. (1991). Anal.chem. Acta,247, 83–87Google Scholar
- Chang, H.N. and Reilly, P.J. (1977)Biotechnol. Bioeng.,19, 923.Google Scholar
- Haemmerli,S., Schaeffler,A.,Manz,A and Widmer,H.M.(1992),Sensors and Actuators,7, 404–407.Google Scholar
- Marques Jr,E.T.A., Spencer Neto,F.A.C., Lima Filho, J.L. (1991),Biochem. Educa.,19, 77–78.Google Scholar
- Park, J., Ro, H. and Kim, H. (1991) Biotechnol. Bioeng.,38, 217–223.Google Scholar
- Hestrin, S., Feingold, D.S. and Shramm, S. (1955)Methods in Enzymology,1, 252–253.Google Scholar
- Raabo, E. and Terkildsen, T.C. (1960), Scand. J. Clin. Lab. Invest.,12, 402–407.Google Scholar
- Weetall, H.H. (1976),Methods in Enzymology 44, 134–148.Google Scholar
- Warburg, O., and Christian, P (1941)Bioch. Zeit.,310, 384Google Scholar
- Watanabe, E., Takagi, M., Takei, S., Hoshi, M. and Shu-gui, C. (1991). Biotecnol. Bioeng.,38, 99–103.Google Scholar