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Biotechnology Techniques

, Volume 9, Issue 5, pp 345–348 | Cite as

Simple and inexpensive Flow Injection Analysis for determination of sucrose using invertase and glucose oxidase immobilised on glass beads

  • V. Leite
  • I. C. Leão
  • G. F. V. de Vasconcelos
  • M. C. B. Pimentel
  • V. L. SIlva
  • E. H. M. Melo
  • J. L. Lima Filho
Article

Abstract

A Flow Injection Analysis (FIA) for sucrose using invertase (E.C. 3.2.1.26), mutarotase (E.C.5.1.3.3) and glucose oxidase (E.C.1.1.3.4) was developed. The enzymes were immobilised on glass beads using glutaraldehyde. The sucrose concentration was related to oxygen saturation. Fall in O2 concentration, as a result of sucrose oxidation, was detected by a low cost, home-made O2 electrode. The system was able to measure sucrose from 0.025 to 100mM with a response time of 6min using 200 μl of sample, with an apparent Km of 42mM of sucrose. The system has been operated satisfactorily for 50 days without loss any initial activity.

Keywords

Oxidation Oxygen Glucose Enzyme Sucrose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • V. Leite
    • 1
    • 2
  • I. C. Leão
    • 1
    • 2
  • G. F. V. de Vasconcelos
    • 1
    • 2
  • M. C. B. Pimentel
    • 1
    • 2
  • V. L. SIlva
    • 1
    • 2
  • E. H. M. Melo
    • 1
    • 2
  • J. L. Lima Filho
    • 1
    • 2
  1. 1.Departamento de Bioquímica/UFPE and Laboratório de Imunopatologia Keizo Asami - LIKA/UFPEUniversidade Federal e PernambucoRecifeBrazil
  2. 2.2-Departamento de Engenharia Química - CT - UFPEUniversidade Federal e PernambucoRecifeBrazil

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