Roux's archives of developmental biology

, Volume 200, Issue 2, pp 113–116 | Cite as

Localized distribution of a novel mesenchyme-specific antigen in developing chick digestive organs

Comparison with the distribution of fibronectin, laminin and tenascin
  • Keiko Takiguchi-Hayashi
  • Sadao Yasugi
Short Communications


The mesenchymes of the two avian stomachs, the proventriculus (glandular stomach) and the gizzard (muscular stomach), exert different inductive influences on stomach epithelial morphogenesis and cytodifferentiation. To search for a molecular difference between these two mesenchymes, we have produced monoclonal antibodies directed against chick proventriculi and gizzards and have screened those that differently recognized proventricular and gizzard mesenchymes. Finally, we obtained one monoclonal antibody, T95, and characterized it immunohistochemically. T95 characteristically stains the mesenchymal region just under the gizzard epithelium from 6 days of incubation onward to about 10 days of incubation, while it stains proventricular mesenchyme only weakly during these stages. We also examined immunohistochemically the distribution of well-known extracellular matrix molecules, such as fibronectin, laminin and tenascin, and none of them showed the same localization as T95 antigen in proventricular and gizzard mesenchymes. These results indicate that T95 will be an interesting marker which distinguishes the proventricular and gizzard mesenchymes, at the time when they have different inductive ability.

Key words

Extracellular matrix Fibronectin Laminin Tenascin Stomach 


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Copyright information

© Springer-Verlag 1991 1991

Authors and Affiliations

  • Keiko Takiguchi-Hayashi
    • 1
  • Sadao Yasugi
    • 1
  1. 1.Zoological Institute, Faculty of ScienceUniversity of TokyoHongo, TokyoJapan

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