Advertisement

Roux's archives of developmental biology

, Volume 200, Issue 2, pp 113–116 | Cite as

Localized distribution of a novel mesenchyme-specific antigen in developing chick digestive organs

Comparison with the distribution of fibronectin, laminin and tenascin
  • Keiko Takiguchi-Hayashi
  • Sadao Yasugi
Short Communications

Summary

The mesenchymes of the two avian stomachs, the proventriculus (glandular stomach) and the gizzard (muscular stomach), exert different inductive influences on stomach epithelial morphogenesis and cytodifferentiation. To search for a molecular difference between these two mesenchymes, we have produced monoclonal antibodies directed against chick proventriculi and gizzards and have screened those that differently recognized proventricular and gizzard mesenchymes. Finally, we obtained one monoclonal antibody, T95, and characterized it immunohistochemically. T95 characteristically stains the mesenchymal region just under the gizzard epithelium from 6 days of incubation onward to about 10 days of incubation, while it stains proventricular mesenchyme only weakly during these stages. We also examined immunohistochemically the distribution of well-known extracellular matrix molecules, such as fibronectin, laminin and tenascin, and none of them showed the same localization as T95 antigen in proventricular and gizzard mesenchymes. These results indicate that T95 will be an interesting marker which distinguishes the proventricular and gizzard mesenchymes, at the time when they have different inductive ability.

Key words

Extracellular matrix Fibronectin Laminin Tenascin Stomach 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 226:550–552Google Scholar
  2. Hayashi K (1987) Thinning of the basement membrane and localized cell proliferation during gland formation of the chick proventriculus. Dev Growth Differ 29:285–295Google Scholar
  3. Hayashi K, Yasugi S, Mizuno T (1988) Pepsinogen gene transcription induced in heterologous epithelial-mesenchymal recombinations of chicken endoderms and glandular stomach mesenchyme. Development 103:725–731Google Scholar
  4. Kedinger M, Simon-Assmann P, Bouziges F, Haffen K (1988) Epithelial-mesenchymal interactions in intestinal epithelial-differentiation. Gastroenterology 23 Suppl 151:62–69Google Scholar
  5. Sariola H, Aufderheide E, Bernhard H, Henke-Fahle S, Dippold W, Ekblom P (1988) Antibodies to cell surface ganglioside GD3 perturb inductive epithelial-mesenchymal interactions. Cell 54:235–245Google Scholar
  6. Sawyer RH, Fallon JF (1983) Epithelial-mesenchymal interactions in development. Praeger, New YorkGoogle Scholar
  7. Steedman HF (1957) Polyester wax. A new ribboning embedding medium for histology. Nature 179:1345Google Scholar
  8. Takiguchi K, Yasugi S, Mizuno T (1986) Gizzard epithelium of chick embryos can express embryonic pepsinogen antigen, a marker protein of proventriculus. Rouxs Arch Dev Biol 195:475–483Google Scholar
  9. Takiguchi K, Yasugi S, Mizuno T (1988a) Developmental changes in the ability to express embryonic pepsinogen in the stomach epithelia of chick embryos. Rouxs Arch Dev Biol 197:56–62Google Scholar
  10. Takiguchi K, Yasugi S, Mizuno T (1988b) Pepsinogen induction in chick stomach epithelia by reaggregated proventricular mesenchymal cellsin vitro. Dev Growth Differ 30:241–250Google Scholar
  11. Takiguchi-Hayashi K, Yasugi S (1990) Transfilter analysis of the inductive influence of proventricular mesenchyme on stomach epithelial differentiation of chick embryos. Rouxs Arch Dev Biol 198:460–466Google Scholar
  12. Thesleff I, Mackie E, Vainio S, Chiquet-Ehrismann R (1987) Changes in the distribution of tenascin during tooth development. Development 101:289–296Google Scholar
  13. Thesleff I, Jalkanen M, Vainio S, Bernheld M (1988) Cell surface proteoglycan expression correlates with epithelial-mesenchymal interaction during tooth morphogenesis. Dev Biol 129:565–572Google Scholar
  14. Wessells NK (1977) Tissue interactions and development. W.A. Benjamin, CaliforniaGoogle Scholar
  15. Yasugi S, Mizuno T (1990) Mesenchymal-epithelial interactions in the organogenesis of digestive tract. Zool Sci 7:159–170Google Scholar
  16. Yasugi S, Matsushita S, Mizuno T (1985) Gland formation induced in the allantoic and small-intestinal endoderm by the proventricular mesenchyme is not coupled with pepsinogen expression. Differentiation 30:47–52Google Scholar

Copyright information

© Springer-Verlag 1991 1991

Authors and Affiliations

  • Keiko Takiguchi-Hayashi
    • 1
  • Sadao Yasugi
    • 1
  1. 1.Zoological Institute, Faculty of ScienceUniversity of TokyoHongo, TokyoJapan

Personalised recommendations