Current Genetics

, Volume 10, Issue 2, pp 87–93 | Cite as

Functional nuclear suppressor of mitochondrialoxi2 mutations in yeast

  • Anna Kruszewska
  • Barbara Szcześniak
Original Articles
  • 30 Downloads

Summary

A semidominant nuclear suppressor, callednam6, ofoxi2-V276 mitochondrial mutation has been isolated and characterized. The nuclear character ofnam6 was proved by its retention inrho° strains, lack of mitotic segregation in diploids and meiotic 2:2 segregation in tetrads. The specificity ofnam6 was tested on 315mit mutations of four mitochondrial genes (oxi1, oxi2, oxi3, andcob-box). It suppresses clearly only three mutations in theoxi2 gene, restoring partially or completely cytochrome aa3 formation. The results suggest a functional character of the suppression.

Key words

Yeast Mitochondria oxi2 mutations Functional suppressors 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Anna Kruszewska
    • 1
  • Barbara Szcześniak
    • 1
  1. 1.Institute of Biochemistry and Biophysics Polish Academy of SciencesWarsawPoland

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