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Effects of thyroxine and propyl-thiouracil on hindlimb regeneration of larvalXenopus laevis

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Abstract

Xenopus laevis larvae at stage 53 and 55 (according to Nieuwkoop and Faber 1956) were subjected to amputation of one or both hindlimbs and reared either in thyroxine (T4) 2.5 to 10 μg/l or in propyl-thiouracil (PTU) 0.01%. Results have shown that when the limb was amputated through a nearly undifferentiated region (tarsalia level, at stage 53) or through a differentiating region (tarsalia level, at stage 55), T4 accelerated the regenerative process and enhanced the mitotic and labelling indices of blastemal cells, when compared with controls. However, PTU delayed the regenerative process and lowered the mitotic and labelling indices. When the limb was amputated through an almost differentiated region (mid-thigh level, at stage 55), T4 inhibited the conic blastema formation, while PTU did not significatively influence limb regeneration. T4 did not modify the morphogenetic properties of the regenerative blastemata, which are characteristic of the developmental stage and the degree of differentiation of the limb tissues at the amputation level. On the whole, the data show that T4, besides being indirectly responsible for the decline of the limb regenerative capacity in a proximodistal direction by promoting limb differentiation, also exerts a direct effect on the regenerative process.

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References

  • Baffoni GM (1960a) Variazioni dell' attività' mitotica e modificazioni cellulari nel prosencefalo e nel mesencefalo di larve di Anfibi anuri trattate con ormone tiroideo. Rend Accad Naz Lincei VIII 28:102–107

    Google Scholar 

  • Baffoni GM (1960b) Contributo allo studio della metamorfosi negli Anfibi. Riv Biol 53:293–340

    Google Scholar 

  • Beaudoin AR (1955) The development of lateral motor column cells in the lumbo-sacral cord inRana pipiens. I: Normal development and development following unilateral limb ablation. Anat Rec 121:81–96

    Google Scholar 

  • Beaudoin AR (1956) The development of lateral motor column cells in the lumbo-sacral cord inRana pipiens. II: Development under the influence of thyroxine. Anat Rec 125:247–259

    Google Scholar 

  • Belkin RI (1950) Influence of thyreoiodine on the regeneration of limbs inAxolotl andAmblystoma. Proc Acad Sci USSR 70:327–329

    Google Scholar 

  • Dent JN (1962) Limb regeneration in larvae and metamorphosing individuals of the South African clawed toad. J Morphol 110:61–77

    Google Scholar 

  • Dournon C, Chibon P (1974) Influence de la temperature, de l'age et des conditions hormonales (thyroxine) sur la proliferération cellulaire chez la jeune larve et pendant la metamorphose du crapaudBufo bufo L. (Amphibien Anoure). Wilhelm Roux' Arch 175:27–47

    Google Scholar 

  • Filoni S, Paglialunga L (1990) Effect of denervation on hindlimb regeneration inXenopus laevis larvae. Differentiation 43:10–19

    Google Scholar 

  • Filoni S, Margotta V, Gibertini G (1974) Effetto della tiroxina sulla rigenerazione del mesencefalo di larve diXenopus laevis (Daudin). Acta Embryol Exper 1:19–41

    Google Scholar 

  • Filoni S, Margotta V, Campanini MG (1979) Sul determinismo del decremento del potere rigenerativo del tetto ottico diXenopus laevis (Daudin) durante la vita larvale. Acta Embryol Exper 1:53–67

    Google Scholar 

  • Filoni S, Bernardini S, Cannata SM (1991) The influence of denervation on grafted hindlimb regeneration of larvalXenopus laevis. J Exp Zool 260:210–219

    Google Scholar 

  • Forsyth JW (1946) The histology of anuran limb regeneration. J Morphol 79:287–321

    Google Scholar 

  • Goldberg JF, Pollack ED (1989) Neuronal overload in the developing anuran lateral motor column in response to limb removal and thyroid hormone. J Comp Neurol 283:578–586

    Google Scholar 

  • Hay ED (1956) Effects of thyroxine on limb regeneration in the newt,Triturus viridescens. Bull Johns Hopkins Hosp 99:262–285

    Google Scholar 

  • Hughes A (1961) Cell degeneration in the larval ventral horn ofXenopus laevis (Daudin). J Embryol Exp Morphol 9:269–284

    Google Scholar 

  • Karczmar AG (1948) Influence of methyl bis (B-chloroethyl)amine (nitrogen mustard), hydroquinone and thyroxine on regeneration in urodele larvae. Anat Rec 101:712

    Google Scholar 

  • Kollros JJ (1961) Mechanisms of amphibian metamorphosis. Am Zool 1:107–114

    Google Scholar 

  • Kollros JJ (1968) Endocrine influences in neural development. In: Wolstenholme G, O'Connor M (eds) Ciba foundation. Symposium on growth of the nervous system. Little Brown, Boston, pp 179–192

    Google Scholar 

  • Liversage RA, Brandes PJ (1982) Forelimb regeneration in thyroidectomized adult newts following organ culture and autografting of the thyroid glands. Wilhelm Roux' Arch 191:149–158

    Google Scholar 

  • Michael MI, Aziz FK (1976) Effect of sodium perchlorate on the restoration of the limb regenerative ability in a metamorphic stage ofBufo regularis Reuss. Folia Biol Krakow 24:309–315

    Google Scholar 

  • Nieuwkoop PD, Faber J (1956) Normal table ofXenopus laevis (Daudin). North-Holland Publ Comp, Amsterdam

    Google Scholar 

  • Pawlowsky EN (1923) Hyperthyroidismus und Regeneration. Wilhelm Roux' Arch 99:620–627

    Google Scholar 

  • Peadon AM (1953) The effects of thiourea on limb regeneration in the tadpole. Growth 17:21–44

    Google Scholar 

  • Pollack ED (1988) Perspectives on development of the lateral motor column in the spinal cord ofRana pipiens larvae. Am J Anat 147:183–192

    Google Scholar 

  • Prestige MC (1965) Cell turnover in the spinal ganglia ofXenopus laevis tadpoles. J Embryol Exp Morphol 13:63–72

    Google Scholar 

  • Prestige MC (1967) The control of cell number in the lumbar spinal ganglia during development ofXenopus laevis tadpoles. J Embryol Exp Morphol 17:453–471

    Google Scholar 

  • Reynolds WA (1963) The effects of thyroxine upon the initial formation of the lateral motor column and differentiation of motor neurons inRana pipiens. J Exp Zool 153:237–249

    Google Scholar 

  • Reynolds WA (1966) Mitotic activity in the lumbosacral spinal cord ofRana pipiens larvae after thyroxine or thiourea treatment. Gen Comp Endocrinol 6:453–564

    Google Scholar 

  • Richardson D (1940) Thyroid and pituitary hormones in relation to regeneration. I. The effect of anterior pituitary hormone on regeneration of the hind leg in normal and thyroidectomized newts. J Exp Zool 83:407–429

    Google Scholar 

  • Romeis B (1913) Der Einfluss verschiedenartiger Ernahrung auf die Regeneration bei Kaulquappen (Rana esculenta): I. Wilhelm Roux' Arch 37:183–216

    Google Scholar 

  • Romeis B (1914) Experimentelle Untersuchungen uber die Wirkung innersekretorischer Organe: I. Der Einfluss von Thyreoideaund Thymusfutterung auf das Wachstum, die Entwicklung, und die Regeneration von Anurenlarven. Wilhelm Roux' Arch 40:571–652

    Google Scholar 

  • Romeis B (1915) Experimentelle Untersuchungen uber die Wirkung innersekretorischer Organe: II. Der Einfluss von Thyreoideaund Thymusfutterung auf das Wachstum, die Entwicklung, und die Regeneration von Anurenlarven. Wilhelm Roux' Arch 41:57–119

    Google Scholar 

  • Rzehak K, Singer M (1966) Limb regeneration and nerve fiber number inRana sylvatica andXenopus laevis. J Exp Zool 162:15–22

    Google Scholar 

  • Schmidt AJ (1958a) Forelimb regeneration of thyroidectomized adult newt. I. Morphology. J Exp Zool 137:197–226

    Google Scholar 

  • Schmidt AJ (1958b) Forelimb regeneration of thyroidectomized adult newt. II. Histology. J Exp Zool 139:95–135

    Google Scholar 

  • Schmidt AJ (1958c) Thyroxine effects on forelimb regeneration in the hypotbyroid adult newtTriturus viridescens. Anat Rec 123:503–504

    Google Scholar 

  • Schotté OE, Harland M (1943) Amputation level and regeneration in limbs of lateRana clamitans tadpoles. J Morphol 73:329–363

    Google Scholar 

  • Schotté OE, Washburn WW (1954) The effects of thyroidectomy on the regeneration of the forelimb inTriturus viridescens. Anat Rec 120:156

    Google Scholar 

  • Sessions SK, Bryant SV (1988) Evidence that regenerative ability is an intrinsic property of limb cells inXenopus. J Exp Zool 247:39–44

    Google Scholar 

  • Singer M (1965) A theory of the trophic nervous control of amphibian limb regeneration, including a re-evaluation of quantitative nerve requirements. In: Kiortsis V, Trampusch HAL (eds) Regeneration in animals and related problems. North-Holland, Amsterdam, pp 20–32

    Google Scholar 

  • Singer M, Kamrin RP, Ashbaugh A (1957) The influence of denervation upon trauma-induced regenerates of the forelimb of the post-metamorphic frog. J Exp Zool 136:35–51

    Google Scholar 

  • Van Stone JM (1964) The relationship of nerve number to regenerative capacity in the developing hind limb ofRana sylvatica. J Exp Zool 155:293–302

    Google Scholar 

  • Warren EA, Bower CM (1939) The influence of normal and induced metamorphosis on hind limb regeneration inRana sylvatica. Anat Rec [Suppl] 73:55–56

    Google Scholar 

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Authors and Affiliations

  1. Dipartimento di Biologia, II Università di Roma “Tor Vergata”, 1-00173, Roma, Italy

    G. La Mesa, S. Bernardini, S. M. Cannata & S. Filoni

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  1. G. La Mesa
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  2. S. Bernardini
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  3. S. M. Cannata
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  4. S. Filoni
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Correspondence to: S. Filoni

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La Mesa, G., Bernardini, S., Cannata, S.M. et al. Effects of thyroxine and propyl-thiouracil on hindlimb regeneration of larvalXenopus laevis . Roux's Arch Dev Biol 203, 205–214 (1994). https://doi.org/10.1007/BF00636336

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  • DOI: https://doi.org/10.1007/BF00636336

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