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Effects of training and anabolic steroids on collagen synthesis in dog heart

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Summary

The effects of endurance training and anabolic steroid (Methandienone 1.5 mg · kg−1 p. o. daily) and their combination on regional collagen biosynthesis and concentration in the hearts of male beagle dogs were studied by measuring prolyl 4-hydroxylase (PH) activity and hydroxyproline (HYP) concentration. The PH (P<0.05) and HYP (P<0.05) were both greater in the subendocardinal layer than in the subepicardium (EPI) of the left ventricular wall in controls, whereas opposite gradients (P<0.05) were observed in the right ventricle. Endurance exercise caused an increase of PH activity in EPI of the left ventricular wall (P<0.01). The HYP concentration increased in both layers of the right ventricle in the exercise plus steroid group (P<0.05). The results suggest that transmural differences exist in the rate of collagen synthesis and concentration in canine cardiac ventricles and that endurance exercise may accelerate collagen synthesis in EPI of the left ventricle and the combination of exercise and anabolic steroid causes an increase in collagen concentration in the right ventricular wall.

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Authors and Affiliations

  1. Department of Sports Medicine, University of Oulu, Deaconess Institute of Oulu, SF-90100, Oulu, Finland

    Timo E. S. Takala

  2. Department of Physiology, University of Oulu, Oulu, Finland

    Timo E. S. Takala, Pauliina Rämö & Raimo Kettunen

  3. Department of Medical Biochemistry, University of Oulu, Oulu, Finland

    Kai Kiviluoma

  4. Research Unit for Sport and Physical Fitness, Jyväskylä, Jyväskylä, Finland

    Veikko Vihko

  5. Department of Clinical Sciences, University of Tampere, Tampere, Finland

    Heikki Kainulainen

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  1. Timo E. S. Takala
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  2. Pauliina Rämö
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  3. Kai Kiviluoma
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  4. Veikko Vihko
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  5. Heikki Kainulainen
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  6. Raimo Kettunen
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Takala, T.E.S., Rämö, P., Kiviluoma, K. et al. Effects of training and anabolic steroids on collagen synthesis in dog heart. Europ. J. Appl. Physiol. 62, 1–6 (1991). https://doi.org/10.1007/BF00635624

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  • DOI: https://doi.org/10.1007/BF00635624

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