Skip to main content

A nuclear protein associated with cell divisions in plants

Planta volume 174, pages 462–472 (1988)Cite this article

Abstract

A nuclear protein, present in carrot meristems and rapidly proliferating cultured cells of carrot (Daucus carota L.) has been identified by the use of a monoclonal antibody (MAb 21D7). By combining the techniques of two-dimensional polyacrylamide gel analysis and blotting separated proteins onto nitrocellulose sheets, it was shown that the antibody detected a single polypeptide of apparent molecular mass (M r) of 45000 and an isoelectric focusing point (pI) of 6.7. This protein was found by subcellular fractionation and immunofluorescence to be highly concentrated in the nucleoli of somatic and zygotic embryos of a wide range of plants. It was not detectable in logarthmically growing cells ofEscherichia coli, yeast, embryos ofDrosophila melanogaster or cultured C3H mouse cells. These data indicate that this protein is a highly conserved non-histone protein associated with nuclei of rapidly dividing plant cells.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Abbreviations

M r :

apparent molecular mass

Da:

dalton

Ig:

immunoglobulins

MAb:

monoclonal antibody

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

2-D gel:

two-dimensional gel electrophoresis

2,4-D:

2,4-dichlorophenoxyacetic acid

References

  • Borkird, C. (1987) Characterization of cDNA clones and the corresponding protein products during development of somatic embryos. Ph.D. thesis, University of California, Berkeley, USA

    Google Scholar 

  • Bradford, M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem.72, 248–252

    Google Scholar 

  • Celis, J.E., Bravo, R., Larsen, P.M., Fey, S.J. (1984) Cyclin, a nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells. Leukemia Res.8, 143–147

    Google Scholar 

  • Choi, J.C., Liu, L., Borkird, C., Sung, Z.R. (1987) Cloning of genes developmentally regulated during plant embryogenesis. Proc. Natl. Acad. Sci. USA84, 1906–1910

    Google Scholar 

  • Choi, J.C., Sung, Z.R. (1984) Two-dimensional gel analysis of carrot somatic embryonic proteins. Plant Molec. Biol. Rep.2, 19–25

    Google Scholar 

  • Chouinard, L.A. (1964) Evidence for the existence of two types of nucleolar ribonucleoproteins in root meristematic cells ofVicia faba. Can. J. Bot.42, 779–785

    Google Scholar 

  • Fazekas de St. Groth, S., Sheidegger, D.J. (1980) Production of monoclonal antibodies; strategy and tactics. J. Immunol. Meth.35, 1–21

    Google Scholar 

  • Feinberg, A., Choi, J.H., Lubich, W.P., Sung, Z.R. (1984) Developmental regulation of polyamine metabolism in growth and differentiation of carrot culture. Planta162, 532–539

    Google Scholar 

  • Fujimura, T., Komamine, A. (1980) Aspects of DNA, RNA and protein synthesis during somatic embryogenesis in a carrotDaucus carota cv. Kurodagosan cell suspension culture. New Phytol.86, 213–218

    Google Scholar 

  • Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res.50, 148–151

    Google Scholar 

  • Jordan, E.G., Chapman, J.M. (1973) Nucleolar and nuclear envelope ultrastructure in relation to cell activity in discs of carrot root (Daucus carota L.). J. Exp. Bot.24, 197–209

    Google Scholar 

  • Karu, A.E., Miller, P.L., Chase, C., Cornutt, W. (1985) A point-addressable transfer system for automated sampling, feeding, and expansion of hybridoma cultures. J. Immunol. Meth.76, 145–156

    Google Scholar 

  • Kearney, J.F., Radbruch, A., Liesgang, B., Rajewoky, K. (1979) A new mouse myeloma cell line which has lost immunoglobulin expression but permits the construction of antibody secretory hybrid cell lines. J. Immunol.123, 1548–1550

    Google Scholar 

  • Kennett, R.H. (1980) Freezing of hybridoma cells. In: Monoclonal Antibodies, p. 204, Kennett, R.H., McKearn, T.J., Bechtol, K., eds. Plenum Press, New York, London

    Google Scholar 

  • Khavkin, E.E., Misharin, S.I., Polikarpochkina, R.T. (1979) Identical embryonal proteins in intact and isolated tissues of maize (Zea mays L.). Planta145, 245–251

    Google Scholar 

  • Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680–685

    Google Scholar 

  • Lischwe, M.A., Ochs, R.L., Reddy, R., Cook, R.G., Yeoman, L.C., Tan, E.M., Reichlin, M., Busch, H. (1985) Purification and partial characterization of a nucleolar scleroderma antigen rich in Ng,Ng-dimethylarginine. J. Biol. Chem.260, 14304–14310

    Google Scholar 

  • Liu, L., Borkird, C., Sung, Z.R. (1985) Molecular cloning of cDNA complementary to poly(A) mRNA differentially expressed during somatic embryogenesis. In: Proc. Somatic Embryogenesis Workshop (San Miniato, Italy), pp. 70–76, Terzi, M., Pitto, L., Sung, Z.R., eds.

  • Loomis, W.F. (1978) The number of developmental genes inDictyostelium. In: Molecular basis of cell-cell interaction, pp. 497–505, Lerner, R., ed., Academic Press, New York, N.Y., USA

    Google Scholar 

  • Morrissey, J.H. (1981) Silver stains for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal. Biochem.117, 307–310

    Google Scholar 

  • O'Farrell, P.J. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem.250, 4007–4021

    Google Scholar 

  • Smith, J.A., Choi, J.H., Krauss, M.R., Karu, A.E., Sung, Z.R. (1985) Monoclonal antibodies that recognize proteins unique to somatic embryos ofDaucus carota. In: Proc. Somatic Embryogenesis Workshop (San Miniato, Italy), pp. 86–94, Terzi, M., Pitto, L., and Sung, Z.R. eds.

  • Smith, J.A., Furner, I., Sung, Z.R. (1981) Nutritional and karyotypic characterization of a haploid cell cutlure ofDaucus carota L. In Vitro17, 315–321

    Google Scholar 

  • Street, H.E. (1968) The induction of cell division in plant suspension cultures. In: Les Cultures de tissus de plantes, pp. 177–193, Street, H.E., ed. Colloques Internationaux du C.N.R.S., Strasbourg, France

  • Stuart, R., Street, H.E. (1969) Studies on the growth in culture of plant cells. 4. The initiation of division in suspensions of stationary phase cells ofAcer pseudoplatanus L. J. Exp. Bot.20, 556–571

    Google Scholar 

  • Sung, Z.R. (1976) Turbidimetric measurement of plant cell culture growth. Plant. Physiol.37, 460–462

    Google Scholar 

  • Sung, Z.R., Feinberg, A., Chorneau, R., Borkird, C., Furner, I., Smith, J., Terzi, M., LoSchiavo, F., Guilian, G., Pitto, L., Nutti-Ronchi, V. (1984) Developmental biology of embryogenesis from carrot cultures. Plant Molec. Biol. Rep.2, 3–14

    Google Scholar 

  • Thimann, K.V. (1977) Hormone action in the whole life of plants. University of Massachusetts Press, Amherst, USA

    Google Scholar 

  • Thomas, T., Wilde, D. (1985) Analysis of gene expression in carrot. In: Proc. Somatic Embryogenesis Workshop (San Miniato, Italy), pp. 77–85, Terzi, M., Pitto, L., Sung, Z.R., eds.

  • Torrey, J.G., Foskett, M. (1970) Cell division in relation to cytodifferentiation in cultured pea root segments. Am. J. Bot.57, 1072–1080

    Google Scholar 

  • Towbin, H., Staehelin, T., Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; procedure and some applications. Proc. Natl. Acad. Sci. USA76, 4350–4354

    Google Scholar 

Download references

Author information

Author notes

  1. J. A. Smith

    Present address: Eni-Chem Americas Biotechnology, Corporate Center, 2000,Princeton Park, 08852, Monmouth Junction, NJ, USA

  2. M. R. Krauss

    Present address: Protein Database, Inc., 405 Oakwood Rd., 11746, Huntington Station, NY, USA

  3. C. Borkird

    Present address: Laboratorium voor Genetica, Rijksuniversiteit, B-9000, Gent, Belgium

Authors and Affiliations

  1. Department of Genetics, University of California, 94720, Berkeley, CA, USA

    J. A. Smith, M. R. Krauss, C. Borkird & Z. Renee Sung

  2. Department of Plant Pathology, University of California, 94720, Berkeley, CA, USA

    J. A. Smith, M. R. Krauss, C. Borkird & Z. Renee Sung

Authors
  1. J. A. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. R. Krauss
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Borkird
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Z. Renee Sung
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Smith, J.A., Krauss, M.R., Borkird, C. et al. A nuclear protein associated with cell divisions in plants. Planta 174, 462–472 (1988). https://doi.org/10.1007/BF00634474

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00634474

Key words

  • Daucus (somatic embryo)
  • Cell division (protein)
  • Embryo (somatic zygotic)
  • Immunofluorescence, indirect
  • Meristem (cell divisions)
  • Monoclonal antibody
  • Nuclear protein (non-histone)
  • Protein (nuclear)