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A nuclear protein associated with cell divisions in plants

Abstract

A nuclear protein, present in carrot meristems and rapidly proliferating cultured cells of carrot (Daucus carota L.) has been identified by the use of a monoclonal antibody (MAb 21D7). By combining the techniques of two-dimensional polyacrylamide gel analysis and blotting separated proteins onto nitrocellulose sheets, it was shown that the antibody detected a single polypeptide of apparent molecular mass (M r) of 45000 and an isoelectric focusing point (pI) of 6.7. This protein was found by subcellular fractionation and immunofluorescence to be highly concentrated in the nucleoli of somatic and zygotic embryos of a wide range of plants. It was not detectable in logarthmically growing cells ofEscherichia coli, yeast, embryos ofDrosophila melanogaster or cultured C3H mouse cells. These data indicate that this protein is a highly conserved non-histone protein associated with nuclei of rapidly dividing plant cells.

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Abbreviations

M r :

apparent molecular mass

Da:

dalton

Ig:

immunoglobulins

MAb:

monoclonal antibody

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

2-D gel:

two-dimensional gel electrophoresis

2,4-D:

2,4-dichlorophenoxyacetic acid

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Smith, J.A., Krauss, M.R., Borkird, C. et al. A nuclear protein associated with cell divisions in plants. Planta 174, 462–472 (1988). https://doi.org/10.1007/BF00634474

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Key words

  • Daucus (somatic embryo)
  • Cell division (protein)
  • Embryo (somatic zygotic)
  • Immunofluorescence, indirect
  • Meristem (cell divisions)
  • Monoclonal antibody
  • Nuclear protein (non-histone)
  • Protein (nuclear)