Summary
We examined the suitability of four reference crops, i.e., two non-fixing trees,Cassia siamea andEucalyptus grandis, and two uninoculated fixing trees,Leucaena leucocephala andAcacia albida, for measuring fixed N2 fixed in inoculatedL. leucocephala andA. albida grown for 36 weeks in pots. The15N isotope-dilution (involving the addition of equal amounts of labelled N fertilizer to the non-fixing and the fixing plants) and theA-value (with different amounts of labelled N fertilizer added to the fixing and the non-fixing crops) methods were used. The isotope dilution approach gave several large negative estimates of fixed N2 inA. albida. Positive and similar values of fixed N2 were measured in all four reference crops using theA-value approach. ForL. leucocephala the isotope-dilution approach gave different estimates of fixed N2, with the different reference crops; the uninoculated N2-fixing crops indicated significantly less fixed N2 than the non-fixing reference crops. Similar values for N2 fixed inL. leucocephala were obtained using the two non-fixing trees, either by the isotope-dilution or theA-value method. On average,A. albida derived about twice as much N from fertilizer asL. leucocephala. In both species, the atom %15N excess declined by about 50% in successive harvests.
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References
Cornet F, Otto C, Rinaudo G, Diem HG, Dommergues Y (1985) Nitrogen fixation byAcacia holosericea grown in field conditions. Acta Oecolog 6:211–218
Danso SKA, Zapata F, Hardarson G (1990) Some misconceptions and practical problems in the use of15n soil enrichment techniques for estimating N2 fixation. Soil Biol Biochem (in preparation)
Dommergues Y (1987) The role of biological nitrogen fixation in agroforestry. In: Agroforestry a decade of development. Steppler HA, Nair PKR (eds) ICRAF — Nairobi, Kenya, pp 245–271
Fiedler R, Proksch G (1975) The determination of nitrogen by emission and mass spectrometry in biochemical analysis: A Review. Anal Chim Acta 78:1–62
Fried M, Middelboe V (1977) Measurement of amount of nitrogen fixed by a legume crop. Plant and soil 47:713–715
Fiied M, Danso SKA, Zapata F (1983) The methodology of measurement of N2 fixation by non-legumes as inferred from field experiments with legumes. Can J Microbiol 29:1053–1062
Gauthier DL, Diem HG, Dommergues YR, Ganry F (1985) Assessment of N2 fixation byCasuarina equisetifolia inoculated withFrankia ORS021 001 using15N methods. Soil Biol Biochem 17:371–379
Ledgard SF, Simpson JR, Freney JR, Bergersen FJ (1985) Effect of reference plant on estimation of nitrogen fixation by subterranean clover using15N methods. Aust J Agric Res 36:663–676
Mohan R, Blane K (1985) Statistical programmes for micro-computers. University of Georgia, USA
Ndoye I, Dreyfus B, Heus A (1988) N2 fixation bySesbania rostrata andSesbania sesban estimated by using15N and total N difference methods. Soil Biol Biochem 20:209–213
Rennie RJ (1982) Quantifying dinitrogen fixation in soybean by15N isotype dilution: The question of the non-fixing control plant. Can J Bot 60:856–861
Ruschel AP, Vose PB, Victoria RL, Salati E (1979) Comparison of isotope techniques and non-nodulating isolines to study the effect of ammonium fertilization on dinitrogen fixation in soybean,Glycine max. Plant and Soil 53:513–525
Sanginga N, Mulongoy K, Ayanaba A (1989) Nitrogen of field inoculatedLeucaena leucocephala (Lam.) de Wit estimated by the nitrogen-15 and the difference method. Plant and Soil 117:269–274
Sanginga N, Zapata F, Danso SKA, Bowen GD (1990a) Effect of successive cuttings on nodulation and nitrogen fixation ofLeucaena leucocephala using dilution and the difference methods. Plant and Soil (in press)
Sanginga N, Bowen GD, Danso SKA (1990b) Nitrogen fixation inLeucaena leucocephala andAcacia albida estimated by the techniques: I. Assessment of genetic variability between and within species provenances. Plant and Soil (in press)
Sougoufara B, Danso SKA, Diem HG, Dommergues YR (1990) Estimating N2 fixation and N derived from soil byCasuarina equisetifolia using15N fertilizer: Some problems and solutions. Soil Biol Biochem (in press)
Vasilas BL, Ham GE (1984) Nitrogen fixation in soybeans: An evaluation of measurement techniques. Argon J 76:759–764
Vincent JM (1970) A manual for the practical study of root nodule bacteria. International Biological Programme Handbook no. 15, Blackwell, Oxford
Wagner GH, Zapata F (1982) Field evaluation of reference crops in the study of nitrogen fixation by legumes using isotope techniques. Agron J 74:607–612
Witty JF (1983) Estimating N-fixation in the field using15N-labelled fertilizer. Some problems and solutions. Soil Biol Biochem 15:631–639
Witty JF, Ritz K (1984) Slow release of15N fertilizer formulation to measure N2-fixation by isotope dilution. Soil Biol Biochem 16:1657–1666
Zapata F, Danso SKA, Hardarson G, Fried M (1987) Time course of nitrogen fixation in field-grown soybean using nitrogen 15 methodology. Agron J 79:172–176
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Sanginga, N., Danso, S.K.A., Zapata, F. et al. Influence of reference trees on N2-fixation estimates inLeucaena leucocephala andAcacia albida using15N-labelling techniques. Biol Fertil Soils 9, 341–346 (1990). https://doi.org/10.1007/BF00634112
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DOI: https://doi.org/10.1007/BF00634112