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Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens

Molecular and General Genetics MGG volume 222, pages 353–360 (1990)Cite this article

Summary

We have searched for induced transcripts in a cDNA library derived from bean cell suspension cultures treated with an elicitor fromColletotrichum lindemuthianum. Six independently isolated cDNAs corresponding to rapidly induced small mRNAs have been classified by their DNA sequence and slightly different induction behaviour into two groups. 5′- and 3′-untranslated regions exhibit little similarity, but the deduced small acidic proteins designated PvPR1 and PvPR2 are 89% identical. No relationship was found with the well-characterized PR1 proteins from tobacco. However, the PvPR proteins are closely related to pI49 in pea (64% identity), pSTH2 in potato (41 % identity) and PcPR1-1 in parsley (39% identity), which are also induced in response to elicitor or microbial attack. Moreover, a major pollen allergen in birch (Betv1) has a 44% identity with PvPR1 proteins. These similarities establish a ubiquitous class of conserved defense-related proteins and suggest a common yet still unknown function. Southern blot analysis indicates that PvPR protein gene organization is highly complex with an estimated copy number of more than 12 genes.

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References

  • Anderson-Prouty AJ, Albersheim P (1975) Host-pathogen interactions. VIII. Isolation of a pathogen-synthesized glucan that elicits a defense response in the pathogens host. Plant Physiol 56:286–291

    Google Scholar 

  • Breiteneder H, Pettenburger K, Bito A, Valenta R, Kraft D, Rumpold H, Scheiner O, Breitenbach M (1989) The gene coding for the major birch pollen allergenBetvl is highly homologous to a pea disease resistance response gene. EMBO J 8:1935–1938

    Google Scholar 

  • Broglie KE, Gaynor JJ, Broglie RM (1986) Ethylene-regulated gene expression: Molecular cloning of the genes encoding endochitinase fromPhaseolus vulgaris. Proc Natl Acad Sci USA 83:6820–6824

    Google Scholar 

  • Corbin DR, Sauer N, Lamb CJ (1987) Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants. Mol Cell Biol 7:6337–6344

    Google Scholar 

  • Cornelissen BJC, Horowitz J, van Kan JAL, Goldberg RB, Bol JF (1987) Structure of tobacco genes encoding pathogenesis-related proteins from the PR1 group. Nucleic Acids Res 15:6799–6811

    Google Scholar 

  • Cramer CL, Ryder TB, Bell JN, Lamb CJ (1984) Rapid switching of plant gene expression induced by fungal elicitor. Science 227:1240–1243

    Google Scholar 

  • Cramer CL, Bell JN, Ryder TB, Bailey JA, Schuch W, Bolwell GP, Robbins MP, Dixon RA, Lamb CJ (1985) Coordinated synthesis of phytoalexin biosynthesis enzymes in biologicallystressed cells of bean (Phaseolus vulgaris L.). EMBO J 4:285–289

    Google Scholar 

  • Daniels CH, Fristensky B, Wagoner W, Hadwiger LA (1987) Pea genes associated with non-host disease resistance toFusarium are also active in race-specific disease resistance toPseudomonas. Plant Mol Biol 8:309–316

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Google Scholar 

  • DeTapia M, Bergmann P, Awade A, Burkard G (1986) Analysis of acid extractable bean leaf proteins induced by mercuric chloride treatment and alfalfa mosaic virus infection. Partial purification and characterization. Plant Sci 45:167–177

    Google Scholar 

  • Dron M, Clouse SD, Lawton MA, Dixon RA, Lamb CJ (1988) Glutathione and fungal elicitor regulation of a plant defense gene promoter in electroporated protoplasts. Proc Natl Acad Sci USA 85:6738–6742

    Google Scholar 

  • Fristensky B, Riggleman R, Wagoner W, Hadwiger LA (1985) Gene expression in susceptible and disease resistant interactions of peas induced withFusarium solani pathogens and chitosan. Physiol Plant Pathol 27:15–28

    Google Scholar 

  • Fristensky B, Horovitz D, Hadwiger LA (1988) cDNA sequences for pea disease resistance response genes. Plant Mol Biol 11:713–715

    Google Scholar 

  • Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25:263–269

    Google Scholar 

  • Kauffmann S, Legrand M, Geoffrey P, Fritig B (1987) Biological function of pathogenesis-related proteins: four PR proteins of tobacco have 1,3-β-glucanase activity. EMBO J 6:3209–3212

    Google Scholar 

  • Hedrick SA, Bell JN, Boller T, Lamb CJ (1988) Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding and infection. Plant Physiol 86:182–186

    Google Scholar 

  • von Heijne G (1985) Signal sequences. The limits of variation. J Mol Biol 184:99–105

    Google Scholar 

  • Lamb CJ, Lawton MA, Dron M, Dixon RA (1989) Signals and transduction mechanisms for activation of plant defenses against microbial attack. Cell 56:215–224

    Google Scholar 

  • Lawton MA, Lamb CJ (1987) Transcriptional activation of plant defense genes by fungal elicitor, wounding and infection. Mol Cell Biol 7:335–341

    Google Scholar 

  • Legrand M, Kauffmann S, Geoffroy P, Fritig B (1987) Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases. Proc Natl Acad Sci USA 84:6750–6754

    Google Scholar 

  • Logemann J, Schell J (1989) Nucleotide sequence and regulated expression of a wound-inducible potato gene (wun1). Mol Gen Genet 219:81–88

    Google Scholar 

  • Lucas J, Camacho Henriquez A, Lottspeich F, Henschen A, Sänger HL (1985) Amino acid sequence of the ‘pathogenesis-related’ leaf protein p14 from viroid-infected tomato reveals a new type of structurally unfamiliar protein. EMBO J 4:2745–2749

    Google Scholar 

  • Matton DP, Brisson N (1989) Cloning, expression and sequence conservation of pathogenesis-related gene transcripts of potato. Mol Plant-Microbe Interac 2:325–331

    Google Scholar 

  • Nassuth A, Sänger HL (1986) Immunological relationship between ‘pathogenesis-related’ leaf proteins from tomato, tobacco and cowpea. Virus Res 4:229–242

    Google Scholar 

  • O'Connell RJ, Bailey JA, Richmond DV (1985) Cytology and physiology of infection ofPhaseolus vulgaris byColletotrichum lindemuthianum. Physiol Plant Pathol 27:75–98

    Google Scholar 

  • Ohashi Y, Matsuoka M (1987) Induction and secretion of pathogenesis-related proteins by salicylate or plant hormones in tobacco suspension cultures. Plant Cell Physiol 28:573–580

    Google Scholar 

  • Pfitzner UM, Goodman HM (1987) Isolation and charaterization of cDNA clones encoding pathogenesis-related proteins from tobacco mosaic virus infected tobacco plants. Nucleic Acids Res 15:4449–4465

    Google Scholar 

  • Redolfi P, Cantisani A, Migheli Q (1989) The pathogenesis-related proteins ofPhaseolus bean. J Phytopathol 126:193–203

    Google Scholar 

  • Richardson M, Valdes-Rodriguez S, Blanco-Labra A (1987) A possible function for thaumatin and a TMV-induced protein suggested by homology to a maize inhibitor. Nature 327:432–434

    Google Scholar 

  • Rigden J, Coutts R (1988) Pathogenesis-related proteins in plants. Trends Genet 4:87–89

    Google Scholar 

  • Schmelzer E, Krüger-Lebus S, Hahlbrock K (1989) Temporal and spatial patterns of gene expression around sites of attempted fungal infection in parsley leaves. Plant Cell 1:993–1001

    Google Scholar 

  • Sharma RC, Barthe JP, Lafitte C, Touzé A (1986) Adsorption in vitro de l'endopolygalacturonase secrétée parColletotrichum lindemuthianum sur des parois de deux lignées de haricots isogéniques résistants et sensibles à l'anthracnose. Can J Bot 64:2903–2908

    Google Scholar 

  • Somssich IE, Schmelzer E, Bollmann J, Hahlbrock K (1986) Rapid activation by fungal elicitor of genes encoding ‘pathogenesis-related’ proteins in cultured parsley cells. Proc Natl Acad Sci USA 83:2427–2430

    Google Scholar 

  • Somssich IE, Schmelzer E, Kawalleck P, Hahlbrock K (1988) Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley. Mol Gen Genet 213:93–98

    Google Scholar 

  • Stanford A, Bevan M, Northcote D (1989) Differential expression within a family of novel wound-induced genes in potato. Mol Gen Genet 215:200–208

    Google Scholar 

  • Templeton MD, Lamb CJ (1988) Elicitors and defence gene activation. Plant Cell Environ 11:395–401

    Google Scholar 

  • Thornburg RW, An G, Cleveland TE, Johnson R, Ryan CA (1987) Wound-inducible expression of a potato inhibitor II. Chloramphenicol acetyltransferase gene fusion in transgenic tobacco plants. Proc Natl Acad Sci USA 84:744–748

    Google Scholar 

  • Van Loon LC (1985) Pathogenesis-related proteins. Plant Mol Biol 4:111–116

    Google Scholar 

  • Van Loon LC, Gerritsen YAM, Ritter CE (1987) Identification, purification and characterization of pathogenesis-related proteins from virus-infected Samsun NN tobacco leaves. Plant Mol Biol 9:593–609

    Google Scholar 

  • Walter MH, Grima-Pettenati J, Grand C, Boudet AM, Lamb CJ (1988) Cinnamyl-alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor. Proc Natl Acad Sci USA 85:5546–5550

    Google Scholar 

  • White RF, Rybicki EP, von Wechmar MB, Dekker JL, Antoniw JF (1987) Detection of PR1-type proteins inAmaranthaceae,Chenopodiaceae,Gramineae andSolanaceae by immunoelectroblotting. J Gen Virol 68:2043–2048

    Google Scholar 

  • Wingate VPM, Lawton MA, Lamb CJ (1988) Glutathione causes a massive and selective induction of plant defense genes. Plant Physiol 86:206–210

    Google Scholar 

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Author notes

  1. Claude Grand

    Present address: RAGT, 18 rue de Séguret Sainerie, B.P. 326, F-12003, Rodez, France

Affiliations

  1. Institut für Planzenphysiologie 260, Universität Hohenheim, P.O. Box 700562, D-7000, Stuttgart 70, Germany

    Michael H. Walter, Jan-Wei Liu & Dieter Hess

  2. The Salk Institute for Biological Studies, Plant Biology Laboratory, P.O. Box 85800, 92138-9216, San Diego, CA, USA

    Michael H. Walter, Claude Grand & Christopher J. Lamb

Authors
  1. Michael H. Walter
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  2. Jan-Wei Liu
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  3. Claude Grand
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  4. Christopher J. Lamb
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  5. Dieter Hess
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Communicated by R.G. Herrmann

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Walter, M.H., Liu, JW., Grand, C. et al. Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens. Mol Gen Genet 222, 353–360 (1990). https://doi.org/10.1007/BF00633840

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  • DOI: https://doi.org/10.1007/BF00633840

Key words

  • PR protein cDNA
  • Fungal elicitor
  • Plant disease resistance
  • Gene family
  • Allergy