Molecular and General Genetics MGG

, Volume 222, Issue 2–3, pp 317–322 | Cite as

Mapping of insertion element IS30 in theEscherichia coli K12 chromosome

  • Masaaki Umeda
  • Efchi Ohtsubo
Article

Summary

We identified seven phage clones containing the insertion element IS30 in a λ phage library mini-set, which includes 476 clones carrying chromosomal segments that cover almost the entire chromosome ofEscherichia coli K12 W3110 (Kohara et al. 1987). We could assign locations and orientations to four copies of IS30 (namedis30A tois30D) on the W3110 chromosome by restriction analysis of phage DNAs containing them. These IS30s were present at the same locations in chromosomes of both W3110 and anotherE. coli K12 strain JE5519, and thus are assumed to be present in otherE. coli K12 derivatives, including early isolates. Among the IS30 copies found, one (is30B) contained a large deletion and possessed only a 181 by stretch of the right terminal region of IS30.

Key words

Insertion sequence Repetitive sequence IS deletion mutant DNA rearrangement 

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References

  1. Bachmann BJ (1983) Linkage map ofEscherichia coli K-12, Edition 7. Microbiol Rev 47:180–230Google Scholar
  2. Birkenbihl RP, Vielmetter W (1989) Complete maps of IS1, IS2, IS3, IS4, IS5, IS30 and IS150 locations inEscherichia coli K12. Mol Gen Genet 220:147–153Google Scholar
  3. Brahma N, Schumacher A, Cullum J, Saedler H (1982) Distribution of theEscherichia coli K12 insertion sequences ISI, IS2 and IS3 among other bacterial species. J Gen Microbiol 128:2229–2234Google Scholar
  4. Caspers P, Dalrymple B, Iida S, Arber W (1984) IS30, a new insertion sequence ofEscherichia coli K12. Mol Gen Genet 196:68–73Google Scholar
  5. Dalrymple B, Caspers P, Arber W (1984) Nucleotide sequence of the prokaryotic mobile genetic element IS30. EMBO J 3:2145–2149Google Scholar
  6. Deonier RC, Hadley RG, Hu M (1979) Enumeration and identification of IS3 elements inEscherichia coli strains. J Bacteriol 137:1421–1424Google Scholar
  7. Galas DJ, Chandler M (1989) Bacterial insertion sequences. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington DC, pp 109–162Google Scholar
  8. Grindley NDF, Reed RR (1985) Transpositional recombination in prokaryotes. Annu Rev Biochem 54:863–896Google Scholar
  9. Hill CW, Harnish BW (1981) Inversions between ribosomal RNA genes ofEscherichia coli. Proc Natl Acad Sci USA 78:7069–7072Google Scholar
  10. Hu M, Deonier RC (1981) Comparison of IS1, IS2 and IS3 copy number inEscherichia coli strains K-12, B and C. Gene 16:161–170Google Scholar
  11. Hu S, Ohtsubo E, Davidson N, Saedler H (1975) Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: Identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. J Bacteriol 122:764–775Google Scholar
  12. Iida S, Meyer J, Arber W (1983) Prokaryotic IS elements. In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 159–221Google Scholar
  13. Kohara Y, Akiyama K, Isono K (1987) The physical map of the wholeE. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508Google Scholar
  14. Louarn JM, Bouché JP, Legendre F, Louarn J, Patte J (1985) Characterization and properties of very large inversions of theE. coli chromosome along the origin-to-terminus axis. Mol Gen Genet 201:467–476Google Scholar
  15. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  16. Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78Google Scholar
  17. Miura K (1967) Preparation of bacterial DNA by the phenol-pH9RNases method. Methods Enzymol 12:543–545Google Scholar
  18. Nisen P, Purucker M, Shapiro L (1979) Deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms. J Bacteriol 140:588–596Google Scholar
  19. Nyman K, Nakamura K, Ohtsubo H, Ohtsubo E (1981) Distribution of the insertion sequence IS1 in Gram-negative bacteria. Nature 289:609–612Google Scholar
  20. Nyman K, Ohtsubo H, Davison D, Ohtsubo E (1983) Distribution of insertion element IS1 in natural isolates of Escherichia coli. Mol Gen Genet 189:516–518Google Scholar
  21. O'Connor MB, Malamy MH (1983) A new insertion sequence, IS121, is found on the Mu dI1 (Aplac) bacteriophage and theEscherichia coli K12 chromosome. J Bacteriol 156:669–679Google Scholar
  22. Ohtsubo H, Ohtsubo E (1977) Repeated DNA sequences in plasmids, phages, and bacterial chromosomes. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 49–63Google Scholar
  23. Ohtsubo H, Ohtsubo E (1978) Nucleotide sequence of an insertion element, IS1. Proc Nail Acad Sci USA 75:615–619Google Scholar
  24. Saedler H, Heiss B (1973) Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome ofE. coli K-12. Mol Gen Genet 122:267–277Google Scholar
  25. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  26. Savić DJ, Romac SP, Ehrlich SD (1983) Inversion in the lactose region ofEscherichia coli K-12: Inversion termini map within IS3 elements α3 β3 and β5, α5. J Bacteriol 155:943–946Google Scholar
  27. Sawyer SA, Dykhuizen DE, DuBose RF, Green L, Mutangadura-Mhlanga T, Wolczyk DF, Hartl DL (1987) Distribution and abundance of insertion sequences among natural isolates ofEscherichia coli. Genetics 115:51–63Google Scholar
  28. Sekine Y, Ohtsubo E (1989) Frameshifting is required for production of the transposase encoded by insertion sequence1. Proc Natl Acad Sci USA 86:4609–4613Google Scholar
  29. Timmons MS, Spear K, Deonier RC (1984) Insertion element IS121 is nearproA in the chromosomes ofEscherichia coli K-12 strains. J Bacteriol 160:1175–1177Google Scholar
  30. Umeda M, Ohtsubo E (1989) Mapping of insertion elements IS1, IS2 and IS3 on theEscherichia coli K-12 chromosome: Role of the insertion elements in formation of Hfrs and F′ factors and in rearrangement of bacterial chromosomes. J Mol Biol 208:601–614Google Scholar
  31. Umeda M, Ohtsubo E (1990) Mapping of insertion element IS5 in theEscherichia coli K-12 chromosome: Chromosomal rearrangements mediated by IS5. J Mol Biol 213, in pressGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Masaaki Umeda
    • 1
  • Efchi Ohtsubo
    • 1
  1. 1.Institute of Applied MicrobiologyUniversity of TokyoTokyoJapan

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