Molecular and General Genetics MGG

, Volume 222, Issue 2–3, pp 317–322 | Cite as

Mapping of insertion element IS30 in theEscherichia coli K12 chromosome

  • Masaaki Umeda
  • Efchi Ohtsubo


We identified seven phage clones containing the insertion element IS30 in a λ phage library mini-set, which includes 476 clones carrying chromosomal segments that cover almost the entire chromosome ofEscherichia coli K12 W3110 (Kohara et al. 1987). We could assign locations and orientations to four copies of IS30 (namedis30A tois30D) on the W3110 chromosome by restriction analysis of phage DNAs containing them. These IS30s were present at the same locations in chromosomes of both W3110 and anotherE. coli K12 strain JE5519, and thus are assumed to be present in otherE. coli K12 derivatives, including early isolates. Among the IS30 copies found, one (is30B) contained a large deletion and possessed only a 181 by stretch of the right terminal region of IS30.

Key words

Insertion sequence Repetitive sequence IS deletion mutant DNA rearrangement 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Masaaki Umeda
    • 1
  • Efchi Ohtsubo
    • 1
  1. 1.Institute of Applied MicrobiologyUniversity of TokyoTokyoJapan

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