Apparent equilibrium constant between β-adrenoceptors and a competitive antagonist on a cultured pacemaker cell of mammalian heart

  • Alberto J. Kaumann
  • Rainer Wittmann


Heart cells from newborn rats and kittens were cultured. In both species the rate of pacemaker activity of single, cultured cells was increased by low concentrations of catecholamines. The β-adrenoceptor blocker (-)-KL255 antagonized competitively the effects of both, (-)-noradrenaline and (-)-isoprenaline. A mean equilibrium constant of 3.6×10−10 M between (-)-KL255 and β-adrenoceptors of single cells was estimated.

Key words

Drug-β-Adrenoceptor Complex Single Heart Cell 


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Alberto J. Kaumann
    • 1
  • Rainer Wittmann
    • 1
  1. 1.Lehrstuhl für Klinische PhysiologiePhysiologisches Institut der Universität DüsseldorfDüsseldorf

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