Summary
54 cultures were established from brain tissue obtained 2–3 hrs after death from 1 case of multiple sclerosis and 30 cultures from another case. Following fusion with indicator cells in the presence of lysolecithin, a parainfluenza type 1 virus (6/94 virus) was isolated from cultures representing one plaque area in the first case and one plaque area in the second case. A cell line chronically infected with the 6/94 virus has been maintained for more than 100 passagesin vitro. A close relationship to the Sendai Hemagglutinating Virus of Japan (HVJ) is indicated from RNA-RNA hybridization and the patterns of electrophoretic mobilities of viral polypeptides. Conversely, differences in optima for growth-requirement temperatures, hemolytic activity and the capability to fuse mammalian cells, distinguishes 6/94 virus and HVJ as distinct phenotypic entities of a closely related genotype.
Zusammenfassung
Aus dem Hirngewebe von 2 Fällen von multipler Sklerose, welches 2–3 Std post mortem auf Kulturen angesetzt werden konnte, wurden je 54 und 30 Gewebekulturen gewonnen. Nach Fusion mit Indicatorzellen in Gegenwart von Lysolecithin wurde ein Parainfluenza Typ 1-Virus (6/94-Virus) aus einem Plaque-Bereich in beiden Fällen isoliert. Eine mit 6/94-Virus chronisch infizierte Zellinie wurde über mehr als 100 Passagenin vitro aufrechterhalten. Eine enge Verwandtschaft zum japanischen hämagglutinierenden Sendai-Virus (HVJ) ergibt sich auf Grund der RNA-RNA-Hybridisation und der elektrophoretischen Wanderung der Virus-Polypeptide. Andererseits zeigen Differenzen der erforderlichen Wachstumstemperaturen, hämolytischen Aktivität und die Fähigkeit zur Fusion von Säugetierzellen, daß es sich bei dem 6/94- und dem HVJ-Virus um verschiedene Phänotypen eines eng verwandten Genotyps handelt.
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Supported in part by funds from the National Multiple Sclerosis Society; USPHS Research Grant NS-11036 from the National Institute of Neurological Disease and Stroke and RR 05540 from the Division of Research Resources; by Deutsche Forschungsgemeinschaft AZ, Me270/10, Schwerpunkt Multiple Sklerose und verwandte Erkrankungen.
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Koprowski, H., ter Meulen, V. Multiple sclerosis and parainfluenza 1 virus. J. Neurol 208, 175–190 (1975). https://doi.org/10.1007/BF00630631
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DOI: https://doi.org/10.1007/BF00630631