European Journal of Clinical Pharmacology

, Volume 21, Issue 3, pp 251–255 | Cite as

Radioimmunoassay of glipizide in human plasma

  • E. Maggi
  • E. Pianezzola
  • G. Valzelli
Originals

Summary

A simple, sensitive radioimmunoassay has been developed for the direct determination of glipizide in human plasma. Antisera raised in rabbits immunized with a glipizide analogue conjugated to bovine serum albumin were highly specific, the two main metabolites, 3,cis-hydroxycyclohexyl derivative and 4,trans-hydroxycyclohexyl derivative, having cross reactivities of 0.73% and 1.66%, respectively. The method can measure amounts as small as 1 ng/ml. The intra- and inter-assay coefficients of variation lay between 2.98–5.79% and 2.35–8.66%, respectively. The mean recovery of glipizide added to plasma was 99–105% over the range 1–500 ng/ml. The method was employed to determine plasma levels in six subjects after administration of a 5 mg tablet of glipizide. The results were in accordance with those found after administration of the same dose of radiolabelled glipizide to two other subjects.

Key words

glipizide radioimmunoassay antibody preparation cross-reactivity precision and accuracy plasma level man 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Fuccella LM, Tamassia V, Valzelli G (1973) Metabolism and kinetics of the hypoglycaemic agent glipizide in man — Comparison with glibenclamide. J Clin Pharmacol 13: 68Google Scholar
  2. 2.
    Goldaniga GC, Maraone C, Pianezzola E, Valzelli G, Ambrogi V (1973) Metabolism of glipizide in the rat and dog and its tissue distribution in the mouse. Arzneim Forsch (Drug Res) 23: 242Google Scholar
  3. 3.
    Balant L, Fabre J, Zahnd GR (1975) Comparison of the Pharmacokinetics of glipizide and glibenclamide in man. Eur J Clin Pharmacol 8: 63Google Scholar
  4. 4.
    Schmidt HA, Shoog M, Schweer KH, Winkler E (1973) Pharmacokinetics and pharmacodynamics as well as metabolism following orally and intravenously administered14C-glipizide, a new antidiabetic. Diabetologia (Suppl) 9: 320Google Scholar
  5. 5.
    Balant L, Zahnd G, Gorgia A, Schwarz R, Fabre J (1973) Pharmacokinetics of glipizide in man: influence of renal insufficiency. Diabetologia (Suppl) 9: 331Google Scholar
  6. 6.
    Farr RS (1958) A quantitative immunochemical measure of the primary interaction between I-BSA and antibody. J Infect Dis 103: 239Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • E. Maggi
    • 1
  • E. Pianezzola
    • 1
  • G. Valzelli
    • 1
  1. 1.Pharmaceutical Research and Development, Pharmacokinetics DepartmentFarmitalia Carlo Erba S. p. A.MilanItaly

Personalised recommendations