European Journal of Clinical Pharmacology

, Volume 21, Issue 3, pp 251–255 | Cite as

Radioimmunoassay of glipizide in human plasma

  • E. Maggi
  • E. Pianezzola
  • G. Valzelli


A simple, sensitive radioimmunoassay has been developed for the direct determination of glipizide in human plasma. Antisera raised in rabbits immunized with a glipizide analogue conjugated to bovine serum albumin were highly specific, the two main metabolites, 3,cis-hydroxycyclohexyl derivative and 4,trans-hydroxycyclohexyl derivative, having cross reactivities of 0.73% and 1.66%, respectively. The method can measure amounts as small as 1 ng/ml. The intra- and inter-assay coefficients of variation lay between 2.98–5.79% and 2.35–8.66%, respectively. The mean recovery of glipizide added to plasma was 99–105% over the range 1–500 ng/ml. The method was employed to determine plasma levels in six subjects after administration of a 5 mg tablet of glipizide. The results were in accordance with those found after administration of the same dose of radiolabelled glipizide to two other subjects.

Key words

glipizide radioimmunoassay antibody preparation cross-reactivity precision and accuracy plasma level man 


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • E. Maggi
    • 1
  • E. Pianezzola
    • 1
  • G. Valzelli
    • 1
  1. 1.Pharmaceutical Research and Development, Pharmacokinetics DepartmentFarmitalia Carlo Erba S. p. A.MilanItaly

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