Abstract
Following a study of the main factors involved in the 68-Ga labelling of human serum albumin microspheres (H.S.A.M.), especially methods of production and preparation of active solution and conditions of radioelement fixation on the protein support, the practical details of a fast technique (60 min) based on the process described by Hnatowich are presented. This method gives high labelling yields (93±3%), and after washing of the microspheres leads to a radiopharmaceutical product almost without free68Ga (less than 2%). The spheres ready for use carry a total radioactivity corresponding to about 35%, including decay, of the activity originally recovered in the generator eluate and to more than 98% of that, found in the final suspension.
The labelled product is sterile, non-pyrogenic and non-toxic. When it is injected in animals by left ventricle catheterization the uptake rates in the heart, lungs, spleen, left kidney and right kidney are similar to those observed with reference85Sr-labelled carbonized microspheres. This radiopharmaceutical, casy to prepared and having excellent biological and nuclear properties, seems ideally suited for the scanning of organs by positron emission tomoscintig raphy.
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Yvert, J.P., Mazière, B., Verhas, M. et al. Simple, fast preparation of gallium-68-labelled human serum albumin microspheres. Eur J Nucl Med 4, 95–99 (1979). https://doi.org/10.1007/BF00626078
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DOI: https://doi.org/10.1007/BF00626078