Advertisement

Blut

, Volume 54, Issue 6, pp 337–342 | Cite as

A rapid method for the preparation of the neutrophil fraction of granulocytes from human blood by centrifugation on isotonic nycodenz gradients

  • T. C. Ford
  • R. Needle
  • D. Rickwood
Original Articles

Summary

A rapid method for the preparation of neutrophils of the granulocyte fraction of human blood is described. A leucocyte-rich fraction is loaded onto preformed isotonic Nycodenz gradients, formed by mixing a stock isotonic Nycodenz solution with a NaCl diluent solution. After low speed centrifugation a neutrophil fraction of high viability, yield and purity can be isolated from the gradients.

Key words

Blood Neutrophils Nycodenz 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Boyum A (1976) Scand J Immunol 5 (Suppl 5): 9Google Scholar
  2. 2.
    Boyum A (1983) Scand J Immunol 17: 429Google Scholar
  3. 3.
    Day RP (1970) Immunol 18: 955Google Scholar
  4. 4.
    Day RP (1972) Clin Allergy 2: 205Google Scholar
  5. 5.
    Ford TC, Rickwood D (1982) Anal Biochem 124: 293Google Scholar
  6. 6.
    Jepsen LV, Skottun T (1982) Scand J Clin Lab Invest 42: 235–238Google Scholar
  7. 7.
    Loos JA, Roos D (1974) Expl Cell Res 86: 333Google Scholar
  8. 8.
    Mutzel W, Speck U (1980) Acta Radiologica (Suppl) 362: 87Google Scholar
  9. 9.
    Rickwood D, Ford TC, Graham J (1982) Anal Biochem 123: 23Google Scholar
  10. 10.
    Rickwood D, Hell A, Birnie GD (1973) FEBS Lett 33: 221Google Scholar
  11. 11.
    Salvesen S (1980) Acta Radiol (Suppl) 362: 73Google Scholar
  12. 12.
    Wakefield JSJ, Gale JS, Berridge MV, Jordan TW, Ford HC (1982) Biochem J 202: 795Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • T. C. Ford
    • 1
  • R. Needle
    • 2
  • D. Rickwood
    • 1
  1. 1.Department of BiologyUniversity of Essex, Wivenhoe ParkColchesterUK
  2. 2.Department of RadiopharmacyColchester General HospitalColchesterUK

Personalised recommendations