Abstract
Specific suppressor T cells (SSTC), primed in vivo with H-2 antigens, have been shown previously to inhibit DNA synthesis in the one-way, three-cell mixed lymphocyte reaction (MLR) provided that (a) the stimulator cells bear the priming H-2 antigens, and (b) the responder cells possessIC+S regions homologous to those of the SSTC. Anti-B10.A BlO.A(2R) SSTC (anti-Dd) and anti-A.AL A.TL SSTC (anti-Kk) are shown here to be able to inhibit the DNA synthesis triggered in MLR, not only by the corresponding antigens, Dd and Kk, respectively, but also by irrelevant, third-party H-2 and Mls products provided that the corresponding and third-party antigens are presented on the same stimulator cell. If stimulatorH-2 regions, whose products interact with SSTC and responders, are located on different stimulator cells within the particular MLR, SSTC activity is not elicited. Participation of cytotoxic T lymphocytes in DNA-synthesis suppression is ruled out. Direct contact or location of the inhibited responder cell very close to SSTC is considered to be required for the development of SSTC activity.
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References
Al-Adra, A. R. and Pilarski, L. M.: Antigen-specific suppression of cytotoxic T cell responses in mice. I. Suppressor T cells are not cytotoxic cells.Eur. J. Immunol. 8: 504–511, 1978
Bach, F. H., Bach, M. L., and Sondel, P. M.: Differential function of major histocompatibility complex antigens in T-lymphocyte cultivation.Nature 259: 273–281, 1976
Brondz, B. D., Karaulov, A. V., and Abronina, I. F.: Evidence for receptors of the specific T suppressor cells immune to the H-2 antigens, and enrichment of these cells by fractionation on target cell monolayer.Mol. Biol. (Mosk.) 13: 1287–1295, 1979
Brondz, B. D., Karaulov, A. V., and Abronina, I. F.: Immunological specificity and kinetics of T-cell suppressor induction by immunization of mice with allogeneic spleen cells.Cytologia (Leningrad) 22: 583–590, 1980a
Brondz, B. D., Karaulov, A. V., Abronina, I. F., and Blandova Z. K.: Biological, immunological and genetic characterization of specific suppressor T cells and their receptors immune to antigens of the H-2 complex. Clonal structure, narrow specificity of receptors and genetic restriction of specific T-suppressor function.Mol. Immunol. 17: 833–849, 1980b
Brondz, B. D., Karaulov, A. V., Abronina, I. F., and Blandova, Z. K.: Requirements for induction of specific suppressor T cells and detection of their H-2 antigen-binding receptors by fractionation on target cell monolayers.Scand. J. Immunol. 13: 517–534, 1981a
Brondz, B. D., Abronina, I. F., Zajceva M. B., and Blandova, Z. K.: la-antigens as markers of specific suppressor T cells immune to antigens of the II-2 complex.Genetika (Mosk.) in press, 1981b
Czitrom, A. A., Sunshine, G. H., and Mitchison, N. A.: Suppression of the proliferative response toH-2D by I-J-subregion gene products.Immunogenetics 11: 97–102, 1980
Eisenthal, A., Nachtigal, D., and Feldman, M.: Induction of allospecific suppressor T cells in vitro.Cell. Immunology 34: 112–124, 1977
Glick, J. L., Lockwood, C., Williams, J., Papermaster, B. W., Burns, A. A. and Dutton, A. C.: Human serum as an economical substitute for foetal bovine serum in lymphoid cell cultures.Transplantation 18: 86–92, 1974
Guttman, R. D.: Mixed leucocyte interactionsuppression generated after alloimmunization.Transplantation 24: 316–324, 1977
Matossian-Rogers, A. and Festenstein, H.: Generation of suppressor cells in mice immunized with M locus-incompatible lymphocytes.Transplantation 23: 316–321, 1977
Möller, G. (ed.):Suppressor T lymphocytes. Transplantation Reviews, Volume 26. Munksgaard, Copenhagen, 1975
Orosz, C. G. and Bach, F. H.: Alloantigen-activated CML suppression independent of cytotoxic activity.J. Immunol. 123: 1419–1421, 1979
Paul, W. E. and Benacerraf, B.: Functional specificity of thymus-dependent lymphocytes.Science 195: 1293–1299, 1977
Rich, S. S. and Rich, R. R.: Regulatory mechanisms in cell-mediated immune responses. III. I-region control of suppressor cell interaction with responder cells in mixed lymphocyte reactions.J. Exp. Med. 143: 672–685, 1976
Rich, S. S., David, C. S., and Rich, R. R.: Regulatory mechanisms in cell-mediated immune responses. VII. Presence of I-C subregion determinants on mixed leukocyte reaction suppressor factor.J. Exp. Med. 149: 114–126, 1979
Rich, S. S. and David, C. S.: Regulatory mechanisms in cell-mediated immune responses. VIII. Differential expression of I-region determinants by suppressor cells and their targets in suppression of mixed leukocyte reactions.J. Exp. Med. 150: 1108–1121, 1979
Snell, G. D.: T cells, T cell recognition structures, and the major histocompatibility complex.Immunol. Rev. 38: 3–69, 1978
Suslov, A. P., Eliseeva, L. S., Brondz, B. D., Abronina, I. F., and Chervonsky, A. V.: Effect of thymectomy and adrenalectomy of adult animals, as well as of serotonin on the functional activities of T lymphocytes immune in vivo and in vitro to antigens of the H-2 complex.Immunology (Mosk.) 1: 74–79, 1980
Truitt, J. A., Rich, R. R., and Rich, S. S.: Regulation of cytotoxic lymphocyte responses in vitro by alloantigen-activated spleen cells.J. Immunol. 119: 31–37, 1977
Turkin, D. and Sercarz, E. E.: Key antigenic determinants in regulation of the immune response.Proc. Natl. Acad. Sci. U.S.A. 74: 3984–3987, 1977
Wagner, H., Starzinski-Powitz, A., Pfizenmaier, K., and Röllinghoff, M.: Regulation of T cell-mediated cytotoxic allograft responses. I. Evidence for antigen-specific suppressor T cells.Eur. J. Immunol. 6: 873–878, 1976
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Brondz, B.D., Karaulov, A.V., Chervonsky, A.V. et al. Requirement for the location of both appropriate and irrelevant H-2 antigens on the same stimulator cell for unspecific DNA-synthesis inhibition by the H-2-antigen-primed, specific suppressor T cells. Immunogenetics 15, 167–176 (1982). https://doi.org/10.1007/BF00621949
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DOI: https://doi.org/10.1007/BF00621949