Summary
Cell cultures derived from adult guinea pig epidermis were subjected to a variety of cytochemical enzyme reactions after up to four months of culture. All cultures exhibited an analogous reaction pattern which remained unchanged throughout the entire observation period. After four months they still corresponded to the pattern encountered in the basal layers of the epidermis. All cells reacted identically; cells with the cytochemical enzyme profile of the corium were not found.
Zusammenfassung
Zellkulturen aus der Epidermis erwachsener Meerschweinchen wurden bis zu einem Alter von 4 Monaten einem Spektrum enzymcytochemischer Reaktionen unterworfen. Alle Stämme reagierten gleichartig, das Reaktionsmuster blieb während des Beobachtungszeitraumes unverändert und entsprach auch nach 4 Monaten noch dem der basalen Schichten der Epidermis. Die Reaktionen der Zellen waren einheitlich; keine Zellen mit der enzymcytochemischen Charakteristik des Coriums wurden vorgefunden.
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Literatur
Barka, T., Anderson, P. J.: Histochemistry, theory, practice and bibliography. New York: Harper and Row, Inc. 1963.
Brody, I.: The Epidermis. In: Normale und pathologische Anatomie der Haut. J. Jadassohn: Handbuch der Haut- und Geschlechtskrankheiten, Ergänzungswerk Bd. I/1, S. 1–142. Berlin-Heidelberg-New York: Springer 1968.
Cruickshank, C. N. D., Cooper, J. R., Hopper, C.: The cultivation of cells from adult epidermis. J. invest. Derm.34, 339–342 (1960).
Fishman, W. H.: Rat sceletal muscleβ-glucuronidase localization by postcoupling technique using Naphthol AS-BI-β-D-glucosiduronic acid and fast dark blue. J. Histochem. Cytochem.12, 306–308 (1964).
Fortelius, P.: Enzyme activity in cultured cells under various influences. A cytochemical approach. Acta path. microbiol. scand., Suppl.164, 1–94 (1963).
Fritsch, P., Diem, E.: Enzymcytochemische Untersuchungen an Zellsuspensionen und Primärkulturen aus Meerschweinchenepidermis. (In Vorbereitung.)
Gazzolo, M., Pruniéras, M.: Culture de longue durée de cellules issues de l'épiderme de cobaye adulte. III. Essai de caractérisation ultrastructurelle. Path. et Biol.17, 251–259 (1969).
Gomori, G.: Microscopic Histochemistry. Chicago: University of Chicago Press 1952.
Grogg, E., Pearse, A. G. E.: A critical study on the histochemical techniques for acid phosphatase, with a description of an azo-dye method. J. Path. Bact.64, 627–636 (1952).
Jacquemont, C., Pruniéras, M.: Culture de longue durée de cellules issues de l'épiderme de cobaye adulte. Il Essai de caractérisation cytoenzymologique. Path. et Biol.17, 243–249 (1969).
Maggi, V., Riddle, P. N.: Histochemistry of tissue culture cells: a study of the effects of freezing and of some fixatives. J. Histochem. Cytochem.13, 310–317 (1965).
Melnick, P. J.: Cytoenzymology and isocymes of cultured cells. Progress in histochemistry and cytochemistry, Vol. 2, No. 1. Stuttgart-Portland: G. Fischer 1971.
Nachlas, M. M., Monis, B., Rosenblatt, D., Seligman, A. M.: Improvement of the histochemical localization of leucine aminopeptidase with a new substratel-leucyl-4-methoxy-2-naphtylamide. J. biophys. biochem. Cytol.7, 261–264 (1960).
—, Tsou, K. C., De Souza, E., Cheng, C. S., Seligman, A. M.: Cytochemical demonstration of succinic dehydrogenase by use of a new p-nitrophenyl substituted ditetrazole. J. Histochem. Cytochem.5, 420–436 (1957).
Pearson, B., Grose, F.: Further histochemical studies of esterases by 5-bromoindoxyl-acetate L. Reference to nonspecific and nonenzymatic hydrolysis. Arch. Path.67, 324–332 (1959).
Pruniéras, M.: La culture de l'épiderme de mammifère adulte. Paris: S.P.E.I. 1965.
—: Interactions between keratinocytes and dendritic cells. J. invest. Derm.52, 1–17 (1969).
—, Delecluse, C., Regnier, M.: Culture de longue durée de cellules issues de l'épiderme de cobaye adulte. I. Potentiel de croissance, caryotype et caractères morphologiques. Path. et Biol.17, 235–241 (1969).
Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatases at a physiologic pH: With special reference to the demonstration of bile canaliculi. Amer. J. clin. Path.72, 13–23 (1963).
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Mit dankenswerter Unterstützung des Fonds zur Förderung der wissenschaftlichen Forschung, Wien.
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Fritsch, P., Diem, E. Das enzymcytochemische Verhalten von Langzeitzellkulturen aus der Meerschweinchenepidermis. Arch. Derm. Forsch. 243, 373–381 (1972). https://doi.org/10.1007/BF00620419
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DOI: https://doi.org/10.1007/BF00620419