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Application of a femtosecond self-sustaining mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy

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Abstract

Developments in ultrafast Ti:sapphire laser technology can be applied in the investigation of nonlinear optical processes. We describe the application of a self-sustaining femtosecond Ti:sapphire laser as an illumination source in the field of confocal laser scanning fluorescence microscopy (LSM). We present spectra for various fluorescent stains under two-photon excitation and present LSM images of stained samples under mode-locked illumination. The potential for such a system as a non-destructive technique for studying live cells in biomedical research is discussed.

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References

  1. R. Roy, P. A. Schulz andA. Walther,Opt. Lett. 12 (1987) 672.

    Google Scholar 

  2. Private communication., Coherent Laser Group, Palo Alto, CA 94303, USA.

  3. P. F. Moulton,J. Opt. Soc. Am. B 3 (1986) 125.

    Google Scholar 

  4. W. H. Glenn,IEEE J. Quantum Electron QE5 (1969) 284.

    Google Scholar 

  5. Private communication., Spectra Physics, Mountain View, CA 94039, USA.

  6. P. F. Curley andA. I. Ferguson,Opt. Lett. 16(5) (1991) 321.

    Google Scholar 

  7. P. F. Curley andA. I. Ferguson,Opt. Commun. 80(5) (1991) 365.

    Google Scholar 

  8. W. L. Peticolas, J. P. Goldsborough andK. E. Rieckhoff,Phys. Rev. Lett. 10(2) (1963) 43.

    Google Scholar 

  9. R. Y. Tsien,Methods Cell Biol. 30, Part B (1989) 127.

    Google Scholar 

  10. J. G. White, W. B. Amos andM. Fordham,J. Cell. Biol. 105 (1987) 41.

    Google Scholar 

  11. W. Denk, J. H. Strickler andW. W. Webb,Science: Reports 248 (1990) 73.

    Google Scholar 

  12. M. Minsky,Rev. Scanning 10 (1988) 128.

    Google Scholar 

  13. M. D. Egger,Rev. Trends Neurosci 12 (1989) 11.

    Google Scholar 

  14. D. A. Parthenopoulos andP. M. Rentzepis,Science: Reports 245 (1989) 843.

    Google Scholar 

  15. D. E. Spence, P. Kean andW. Sibbett,Opt. Lett. 16 (1991) 42.

    Google Scholar 

  16. G. T. Maker andA. I. Ferguson,Opt. Lett. 15 (1990) 375.

    Google Scholar 

  17. G. P. A. Malcolm andA. I. Ferguson,Opt. Commun. 82(3) (1991) 299.

    Google Scholar 

  18. J. Harrison, A. Finch, D. M. Rines, G. A. Rines andP. F. Moulton,Opt. Lett. 16(8) (1991) 581.

    Google Scholar 

  19. Ch. Spielmann, F. Krausz, T. Brabec, E. Witner andA. J. Schmidt,Opt. Lett. 16(15) (1991) 1180.

    Google Scholar 

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Authors and Affiliations

  1. Department of Physics and Applied Physics, University of Strathclyde, John Anderson Building, 107 Rottenrow, G4 0NG, Glasgow, UK

    P. F. Curley & A. I. Ferguson

  2. Laboratory of Molecular Biology, Medical Research Council, Hills Road, CB2 2QH, Cambridge, UK

    J. G. White & W. B. Amos

Authors
  1. P. F. Curley
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  2. A. I. Ferguson
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  3. J. G. White
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  4. W. B. Amos
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Curley, P.F., Ferguson, A.I., White, J.G. et al. Application of a femtosecond self-sustaining mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy. Opt Quant Electron 24, 851–859 (1992). https://doi.org/10.1007/BF00620198

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  • DOI: https://doi.org/10.1007/BF00620198

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