Journal of comparative physiology

, Volume 148, Issue 4, pp 491–501 | Cite as

FMRFamide catch contractures of a molluscan smooth muscle: Pharmacology, ionic dependence and cyclic nucleotides

  • S. D. Painter
Article

Summary

  1. 1.

    Both acetylcholine (A Ch) and the molluscan neuropeptide FMRFamide (Phe-Met-Arg-Phe-NH2) set catch in the anterior byssus retractor muscle (ABRM); catch is relaxed by 5-hydroxytryptamine (5 HT).

     
  2. 2.

    The threshold for FMRFamide contracture is 3- to 30-fold lower than for ACh; and low to moderate doses of FMRFamide are more potent than equivalent doses of ACh. At very high concentrations, however, FMRFamide contractures are less forceful than those to ACh.

     
  3. 3.

    FMRFamide contractures are diminished less than ACh contractures in Na-free medium, but contractures are phasic in either case.

     
  4. 4.

    FMRFamide contractures decline much more slowly than ACh contractures in Ca-free ASW and La-ASW. Thus, FMRFamide may have access to a more slowly exchanging pool of intracellular Ca; alternatively, the mechanisms coupling excitation and contraction may differ for the two agonists, with the FMRFamide coupling mechanism being less sensitive to Ca depletion.

     
  5. 5.

    Neither FMRFamide nor ACh effect cAMP levels in the ABRM; and no effect on cGMP levels was detected.

     
  6. 6.

    Cyclic AMP is increased by 5 HT and Na-free ASW, but not by 8-bromoguanosine 3′∶5′-cyclic monophosphate. Thus, relaxation is not always accompanied by increased cAMP levels.

     
  7. 7.

    Immunoreactive FMRFamide is present in the ABRM; thus, its actions are of physiological as well as pharmacological interest.

     

Keywords

cAMP Level cGMP Level FMRFamide Cyclic Monophosphate Increase cAMP Level 

Abbreviations

ABRM

anterior byssus retractor muscle

ACh

acetylcholine

5HT

5-hydroxytryptamine

FMRFamide

Phe-Met-Arg-Phe-NH2

YGG-FMRFamide

Try-Gly-Gly-Phe-Met-Arg-Phe-NH2

cAMP

adenosine 3′∶5′-cychic monophosphate

cGMP

guanosine 3′∶5′-cyclic monophosphate

8-Br-cAMP

8-bromoadenosine 3′∶5′-cyclic monophosphate

8-Br-cGMP

8-bromoguanosine 3′∶5′-cyclic monophosphate

IBMX

3-isobutyl-1-methylxanthine

dTC

d-tubocurarine chloride

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References

  1. Achazi RK, Dölling B, Haakshorst R (1974) 5-HT-induzierte Erschlaffung und cyclisches AMP bei einem glatten Molluskenmuskel. Pflügers Arch 349:19–27Google Scholar
  2. Baguet F (1977) Cyclic-AMP and relaxation mechanism in molluscan catch muscle (ABRM). In: Casteels R, Godfraind T, Rüegg JC (eds) Excitation-contraction coupling in smooth muscle. Elsevier/North Holland Biomedical Press, Amsterdam, pp 407–414Google Scholar
  3. Blaustem MP (1974) The interrelationship between sodium and calcium fluxes across cell membranes. Rev Physiol Biochem Pharmacol 70:33–81Google Scholar
  4. Bolton TB (1979) Mechanisms of action of transmitters and other substances in smooth muscle. Physiol Rev 59:606 718Google Scholar
  5. Cole RA, Twarog BM (1972) Relaxation of catch in a molluscan smooth muscle-I. Effects of drugs which act on the adenyl cyclase system. Comp Biochem Physiol [A] 43:321–330Google Scholar
  6. Diamond J (1978) Role of cyclic nucleotides in control of smooth muscle contraction. Adv Cyclic Nucleotide Res 9:327–340Google Scholar
  7. Dölling B, Achazi RK, Zebe E, Ahlert U (1972) 3′,5′-Adenosinmonophosphat im Byssusretraktormuskel der MiesmuschelMytilus edulis. Naturwissenschaften 59:313Google Scholar
  8. Greenberg MJ, Price DA (1979) FMRFamide, a cardioexcitatory neuropeptide of molluscs: An agent in search of a mission. Am Zool 19:163–174Google Scholar
  9. Hidaka T, Goto M (1973) On the relationship between membrane potential and tension inMytilus smooth muscle. J Comp Physiol 82:357–364Google Scholar
  10. Higgins WJ (1974) Intracellular actions of 5-hydroxytryptamine on the bivalve myocardium. I. Adenylate and guanylate cyclases. J Exp Zool 190:99–110Google Scholar
  11. Ishikawa T, Murakami H, Iwayama Y (1981) Changes in cAMP and cGMP levels induced by relaxing drugs in acetylcholine- and potassium-treated molluscan smooth muscle. Comp Biochem Physiol [C] 70:171–176Google Scholar
  12. Koch RA, Greenberg MJ (1981) Calcium fluxes accompanying the action of 5-hydroxytryptamine on mussel hearts. Comp Biochem Physiol [C] 70:229–239Google Scholar
  13. Köhler G, Lindl T (1980) Effects of 5-hydroxytryptamine, dopamine and acetylcholine on accumulation of cyclic AMP and cyclic GMP in the anterior byssus retractor muscle ofMytilus edulis L. (Mollusca). Pflügers Arch 383:257–262Google Scholar
  14. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  15. Meyer RB, Jr, Miller JP (1974) Minireview: Analogs of cyclic AMP and cyclic GMP: General methods of synthesis and the relationship of structure to enzymic activity. Life Sci 14:1019–1040Google Scholar
  16. Muneoka Y, Twarog BM (1977) Lanthanum block of contraction and relaxation in response to serotonin and dopamine in molluscan catch muscle. J Pharmacol Exp Ther 202:601–609Google Scholar
  17. Muneoka Y, Twarog BM, Mikawa T (1978) Relaxation ofMytilus smooth muscle in low sodium. Comp Biochem Physiol [C] 61:267–273Google Scholar
  18. Napoli SA, Gruetter CA, Ignarro LJ, Kadowitz PJ (1980) Relaxation of bovine coronary arterial smooth muscle by cyclic GMP, cyclic AMP and analogs. J Pharmacol Exp Ther 212:469–473Google Scholar
  19. Painter SD, Greenberg MJ (1981) The action of FMRFamide on a molluscan smooth muscle: Separating the catch from the contracture. J Gen Physiol 78:24a-25aGoogle Scholar
  20. Painter SD, Price DA, Greenberg MJ (1980) FMRFamide and acetylcholine “catch” contractures of a molluscan smooth muscle: Different calcium dependencies. Physiologist 23:40Google Scholar
  21. Painter SD, Morley JS, Price DA (1982) FMRFamide structureactivity relations on some molluscan muscles. Life Sci (in press)Google Scholar
  22. Price DA (1982) The FMRFamide-like peptide ofHelix aspersa. Comp Biochem Physiol [C] 72:325–328Google Scholar
  23. Price DA, Greenberg MJ (1977) Structure of a molluscan cardioexcitatory neuropeptide. Science 197:670–671Google Scholar
  24. Schultz G, Hardman JG, Schultz K, Baird CE, Sutherland EW (1973) The importance of calcium ions for the regulation of guanosine 3′∶5′-cyclic monophosphate levels. Proc Natl Acad Sci USA 70:3889–3893Google Scholar
  25. Schultz K, Böhme E, Kreye VAW, Schultz G (1979) Relaxation of hormonally stimulated smooth muscular tissues by the 8-bromo derivative of cyclic GMP. Naunyn-Schmiedeberg's Arch Pharmacol 306:1–9Google Scholar
  26. Schultz KD, Schultz K, Schultz G (1977) Sodium nitroprusside and other smooth muscle-relaxants increase cyclic GMP levels in rat ductus deferens. Nature 265:750–751Google Scholar
  27. Sugi H, Suzuki S (1978) The nature of potassium- and acetylcholine-induced contractures in the anterior byssal retractor muscle ofMytilus edulis. Comp Biochem Physiol [C] 61:275–279Google Scholar
  28. Sugi H, Yamaguchi T (1976) Activation of the contractile mechanism in the anterior byssal retractor muscle ofMytilus edulis. J Physiol (Lond) 257:531–547Google Scholar
  29. Twarog BM (1954) Responses of a molluscan smooth muscle to acetylcholine and 5-hydroxytryptamine. J Cell Comp Physiol 44:141–163Google Scholar
  30. Twarog BM (1959) The pharmacology of a molluscan smooth muscle. Br J Pharmacol 14:404–407Google Scholar
  31. Twarog BM (1967) Factors influencing contrature and catch inMytilus smooth muscle. J Physiol (Lond) 192:847–856Google Scholar
  32. Twarog BM (1977) Dissociation of calcium dependent reactions at different sites: Lanthanum block of contraction and relaxation in a molluscan smooth muscle. In: Casteels R, Godfraind T, Rüegg JC (eds) Excitation-contraction coupling in smooth muscle. Elsevier/North Holland Biomedical Press, Amsterdam, pp 261–271Google Scholar
  33. Twarog BM (1979) The nature of catch and its control. In: Nachmias VT (ed) Motility in cell function. Academic Press, New York, pp 231–241Google Scholar
  34. Twarog BM, Muneoka Y (1973) Calcium and the control of contraction and relaxation in a molluscan catch muscle. Cold Spring Harbor Symp Quant Biol 37:489–503Google Scholar
  35. Twarog BM, Muneoka Y, Ledgere M (1977) Serotonin and dopamine as neurotransmitters inMytilus: Block of serotonin receptors by an organic mercurial. J Pharmacol Exp Ther 201:350–356Google Scholar
  36. Wilkens LA (1972) Electrophysiological studies on the heart of the bivalve mollusc,Modiolus demissus. I. Ionic basis of the membrane potential. J Exp Biol 56:272–291Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • S. D. Painter
    • 1
  1. 1.Department of Biological ScienceFlorida State UniversityTallahasseeUSA

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