Journal of comparative physiology

, Volume 96, Issue 4, pp 343–356 | Cite as

Spectral selectivity of visual response alterations mediated by interconversions of native and intermediate photopigments inDrosophila

  • William S. Stark
Article

Summary

Recent studies have indicated that intense short wavelength stimulation in flies converts rhodopsin to a longlived metarhodopsin while decreasing visual sensitivity and electroretinographic (ERG) responsivity. Long wavelength stimulation reverses both visual pigment and ERG alterations. In this study of ERG's in white-eyedDrosophila, spectral sensitivities were obtained following intense visible and ultraviolet short wavelength stimuli. Both stimuli decreased sensitivity to all wavelengths while ultraviolet light also selectively decreased ultraviolet sensitivity (Fig. 1). These results isolated three sensitivity components contributing to the ERG in flies: (1) the dark adapted sensitivity (Fig. 1); (2) the residual sensitivity remaining subsequent to intense ultraviolet stimulation (Fig. 1); and (3) the ultraviolet sensitivity specifically abolished by intense ultraviolet stimulation (Fig. 2). Further evidence shows that the three components are probably receptor-specific; the first two resemble recent fly receptor spectral sensitivity data (e.g. Eckert, 1971) while the third represents a separate ultraviolet receptor. Linear reciprocity of time and intensity to alter the ERG responsivity was found over considerable ranges for long wavelength (Fig. 3) and short wavelength (Fig. 4) induced responsivity alterations. ERG action spectra were obtained for altering responsivity (Fig. 5). The action spectrum for decreasing responsivity was roughly parallel with the dark adapted spectral sensitivity for wavelengths below 500 nm. The action spectrum for reestablishing responsivity had a peak near 570 nm and agreed with previous determinations of spectral characteristics of fly metarhodopsin. The action spectra determined were probably based on photopigment interconversions in the 1–6 receptor system. Long wavelength reconversion of metarhodopsin to rhodopsin may explain the high ultraviolet and low red sensitivities and the functional significance of red eye color pigments in flies.

Keywords

Spectral Sensitivity Action Spectrum Visual Pigment Spectral Selectivity Wavelength Stimulus 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • William S. Stark
    • 1
  1. 1.Department of PsychologyThe Johns Hopkins UniversityBaltimoreUSA

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