Journal of Comparative Physiology A

, Volume 157, Issue 4, pp 519–528 | Cite as

The involvement of a rhodopsin-like photopigment in the photoperiodic response of the Japanese quail

  • R. G. Foster
  • B. K. Follett


A technique has been developed for the investigation of the photopigment involved in the photoperiodic control of reproduction in Japanese quail,Coturnix coturnix. When these photoreceptors were exposed to white or monochromatic light a clear relationship was found between light intensity and the extent of photo-induced luteinizing hormone (LH) secretion. A spectroradiometric investigation of the passage of light through the skull and brain enabled us to illuminate the hypothalamic region with equal numbers of photons at a range of wavelengths. Action spectra were then conducted and showed a photopigment with a peak sensitivity at wavelengths near 500 nm. An excellent match was obtained when the standard absorption spectrum for a rhodopsin was fitted to the action spectrum, suggesting a rhodopsin maximally sensitive at 492 nm. The absolute sensitivity of the photoreceptors was calculated at a range of wavelengths: with light at 500 nm, 2.85×10−12 μE·cm−2·s−1 triggered the photoperiodic response. This level of sensitivity is matched only by the rhodopsin visual pigments.


Absorption Spectrum Light Intensity Luteinizing Hormone Clear Relationship Action Spectrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



luteinizing hormone




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  1. Anderson KV, Lemmon V, O'Steen WK (1980) Neurophysiological properties of visual neurones in rats with light damaged retinas. In: Williams TP, Baker BN (eds) Effects of constant light on visual processes. Plenum Press, New York, pp 99–133Google Scholar
  2. Antonini E, Brunori M (1971) Haemoglobin and myoglobin in their reactions with ligands. In: Neuberger A, Tatum EL (eds) Frontiers of biology, vol 21. North Holland, Amsterdam, pp 13–54Google Scholar
  3. Benoit J (1935) Stimulation par la lumière artificielle du développement testiculaire chez les canards aveuglés par section du nerf optique. CR Soc Biol (Paris) 120:133–36Google Scholar
  4. Benoit J (1964) The role of the eye and of the hypothalamus in the photostimulation of gonads in the duck. Ann NY Acad Sci 117:204–216Google Scholar
  5. Benoit J (1974) Rôle de l'oeil dans la gonadostimulation par la lumière chez les oiseaux. Ann Biol Anim Biochem Biophys 13:187–195Google Scholar
  6. Binkley S (1980) Functions of the pineal gland. In: Epple E, Stetson MH (eds) Avian endocrinology. Academic Press, New York, pp 53–74Google Scholar
  7. Clayton RK (1970) Light and Living Matter, vol 1. The physical part. McGraw-Hill, New YorkGoogle Scholar
  8. Dartnall HJA (1975) Assessing the fitness of visual pigments for their photic environment. In: Ali MA (ed) Vision in fishes. Plenum Press, New York, pp 543–563Google Scholar
  9. Deguchi T (1981) Rhodopsin-like photosensitivity of isolated chicken pineal gland. Nature 290:702–704Google Scholar
  10. Follett BK (1984) Birds. In: Lamming GE (ed) Marshall's physiology of reproduction (4th edn). Churchill Livingstone, Edinburgh, pp 283–350Google Scholar
  11. Follett BK, Davies DT, Magee V (1975) The rate of testicular development in Japanese quail (Coturnix coturnix japonica) following stimulation of the extra-retinal photoreceptor. Experientia 31:48–49Google Scholar
  12. Follett BK, Milette JJ (1982) Photoperiodism in quail; testicular growth and maintenance under skeleton photoperiods. J Endocrinol 93:83–90Google Scholar
  13. Follett BK, Scanes CG, Cunningham FJ (1972) A radioimmunoassay for avian LH. J Endocrinol 52:359–378Google Scholar
  14. Glass JD, Lauber JK (1981) Sites and action spectra for encephalic photoreceptors in the Japanese quail. Am J Physiol 240:R220-R228Google Scholar
  15. Hartwig H-G, Oksche H (1982) Neurobiological aspects of extraretinal photoreceptive systems: Structure and function. Experientia 38:991–996Google Scholar
  16. Hartwig H-G, Veen T van (1979) Spectral characteristics of visible radiation penetrating into the brain and stimulating extra-retinal photoreceptors. J Comp Physiol 120:277–282Google Scholar
  17. Homma K, Sakakibara Y (1971) Encephalic photoreceptors and their significance in photoperiodic control of sexual activity in Japanese quail. In: Menaker (ed) Biochronometry. Natl Acad Sci USA, Washington, pp 333–341Google Scholar
  18. Homma K, Sakakibara Y, Ohta M (1977) Potential sites and action spectra for encephalic photoreception in the Japanese quail. In: Follett BK (ed) First int symp avian endocrinol University College of North Wales, pp 25–26Google Scholar
  19. Homma K, Ohta M, Sakakibara Y (1980) Surface and deep photoreceptors in photoperiodism in birds. In: Tanabe Y, Tanaba K, Ookawa T (eds) Biological rhythms in birds. Springer, Berlin Heidelberg New York, pp 149–156Google Scholar
  20. Kato M, Shinzawa K, Yoshikawa S (1981) Cytochrome oxidase is a possible photoreceptor in mitochondria. Photochem Photobiophys 2:263–269Google Scholar
  21. Land MF (1981) Optics and vision in invertebrates. In: Autrum H (ed) Vision in invertebrates (Handbook of sensory physiology, vol VII/B). Springer, Berlin Heidelberg New York, pp 472–592Google Scholar
  22. Lythgoe JN (1979) Ecology of vision. Clarendon Press, OxfordGoogle Scholar
  23. Lythgoe JN (1980) Vision in fishes ecological adaptations. In: Ali MA (ed) Environmental physiology of fishes. Plenum, New York, pp 431–445Google Scholar
  24. MacFarland MN, Munz FW (1975) The visible spectrum during twilight and its implication to vision. In: Evans GC, Bainbridge R, Rackham O (eds) Light as an ecological factor: II. Blackwell, Oxford, pp 249–270Google Scholar
  25. Mason HS, Ingram DJE, Allen B (1960) The free radical, property of melanins. Arch Biochem Biophys 86:225–230Google Scholar
  26. Nelson RJ, Zucker I (1981) Absence of extraocular photoreception in diurnal and nocturnal rodents exposed to direct sunlight. Comp Biochem Physiol 69A:145–148Google Scholar
  27. Nicholls TJ, Follett BK, Robinson JE (1983) A photoperiodic response in gonadectomized Japanese quail exposed to a single long day. J Endocrinol 97:121–126Google Scholar
  28. Oishi T, Kato M (1968) Pineal organ as a possible photoreceptor; photoperiodic testicular responses in Japanese quail. Mem Fac Sci Kyoto Univ 2:12–18Google Scholar
  29. Oishi T, Lauber JK (1973) Photoreception in the photosexual response of quail. II: Effect of intensity and wavelength. Am J Physiol 225:880–886Google Scholar
  30. Oliver J, Baylé JD (1982) Brain photoreceptors for the photoinduced testicular response in birds. Experientia 38:1021–1029Google Scholar
  31. Oliver J, Jallageas M, Baylé JD (1979) Plasma testosterone and LH levels in male quail bearing hypothalamic lesions or radioluminous implants. Neuroendocrinology 28:114–122Google Scholar
  32. Oliver J, Jallageas M, Sicard B, Baylé JD (1980) Testicular responses to local photostimulation of the lobus paraolfactorius in quail. J Physiol (Paris) 76:611–616Google Scholar
  33. Ralph CL (1978) Pineal control of reproduction: non-mammalian vertebrates. Prog Reprod Biol 4:30–50Google Scholar
  34. Shear CR (1980) Alterations in muscle and retinal cell structure after exposure to light. In: William TP, Baker BN (eds) The effects of constant light on visual processes. Plenum, New York, pp 51–73Google Scholar
  35. Sicard B, Oliver J, Baylé JD (1983) Gonadotropic and photosensitive abilities of the lobus paraolfactorius: electrophysiological study in quail. Neuroendocrinology 36:81–87Google Scholar
  36. Simpson SM, Urbanski HF, Robinson JE (1983) The pineal gland and the photoperiodic control of luteinizing hormone secretion in intact and castrated Japanese quail. J Endocrinol 99:281–287Google Scholar
  37. Siopes TD, Wilson WO (1974) Extraocular modification of photoreception in intact and pinealectomisedCoturnix. Poultry Sci 53:2035–2041Google Scholar
  38. Siopes TD, Wilson WO (1980) Participation of the eyes in the photosexual response of Japanese quail. Biol Reprod 23:352–357Google Scholar
  39. Underwood H (1975) Retinally perceived photoperiod does not influence subsequent testicular regression in house sparrows. Gen Comp Endocrinol 27:475–478Google Scholar
  40. Vigh B, Vigh-Teichman I, Rohlich P, Aros B (1982) Immunoreactive opsin in the pineal organ of reptiles and birds. Z Mikrosk Anat Forsch 96:113–119Google Scholar
  41. Vollrath L (1981) The pineal organ. Handbuch der Mikroskopischen Anatomic des Menschen. Springer, Berlin Heidelberg New YorkGoogle Scholar
  42. Wolbarsht ML, Stopp PE, Goodson JE (1964) Ophthalmoscopy of pigeons using transillumination. Experientia 15:466–467Google Scholar
  43. Woodard AE, Moore JA, Wilson WO (1969) Effect of wavelength of light on growth and reproduction in Japanese quail (Coturnix coturnix japonica). Poultry Sci 48:118–123Google Scholar
  44. Yokoyama K, Oksche A, Darden TR, Farner DS (1978) The sites of encephalic photoreception in the photoperiodic induction of growth of the testes in the white-crowned sparrow,Z. l. gambelii. Gen Comp Endocrinol 30:528–533Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. G. Foster
    • 1
  • B. K. Follett
    • 1
  1. 1.AFRC Research Group on Photoperiodism & Reproduction, Department of ZoologyThe UniversityBristolUK

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