Journal of comparative physiology

, Volume 131, Issue 1, pp 13–21 | Cite as

Melanophore potentials of the chromatically intact spinal stoneloach (Noemacheilus barbatulus L.) following adaptation to varying backgrounds

  • C. S. Collis


  1. 1.

    The transient colour response of the stoneloach (Noemacheilus barbatulus) is similar to that described for some other freshwater teleosts.

  2. 2.

    A method is described for measuring the melanophore transmembrane potentials resulting from direct activity of the intact chromatic system.

  3. 3.

    Using this method the melanophore index and the melanophore potential at the cell centrosphere were measured in fish adapted to black and white backgrounds and during background reversal (Figs. 5, 6).

  4. 4.

    The amount of pigment movement for the same change in centrosphere potential is greater in melanophores that are aggregating than in those that are dispersing.

  5. 5.

    The melanophore index and the melanophore potential at the cell centrosphere were measured in fish subjected to darkness following adaptation to black and white backgrounds (Fig. 7), and in fish subjected to illumination on black and white backgrounds after adaptation in darkness (Fig. 8).

  6. 6.

    The melanophore index and the melanophore potential both at the cell centrosphere and at the edge of the pigment were measured in fish adapted to backgrounds of varying reflectivity (Table 1). In all positions of the pigment the sum of these potentials remained nearly constant, although the potentials themselves varied (Figs. 9, 10).



Colour Direct Activity Transmembrane Potential White Background Colour Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Derived Ostwald Index (see Table 1)


Melanophore Index


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  1. Bartlett, M.S.: Fitting a straight line when both variables are subject to error. Biometrics5, 207–212 (1949)Google Scholar
  2. Castrucci, A.M. de L.: Chromatophores of the teleostTilapia melanopleura-II. The effects of chemical mediators, microtubuledisrupting drugs and ouabain. Comp. Biochem. Physiol.50 A, 457–462 (1975)Google Scholar
  3. Davies, R.G.: Computer programming in quantitative biology. London: Academic Press 1971Google Scholar
  4. Egner, O.: Zur Physiologie der Melanosomenverlagerung in den Melanophoren vonPterophyllum scalare Cuv. u. Val.. Cytobiologie4, 262–292 (1971)Google Scholar
  5. Freeman, A.R., Connell, P.M., Fingerman, M.: An electrophysiological study of the red chromatophore of the prawn,Palaemonetes: Observations of the action of red pigment-concentrating hormone. Comp. Biochem. Physiol.26, 1015–1030 (1968)Google Scholar
  6. Frisch, K. von: Beiträge zur Physiologie der Pigmentzellen in der Fischhaut. Pflügers Arch. Ges. Physiol.138, 319–387 (1911)Google Scholar
  7. Fujii, R.: Mechanism of ionic action in the melanophore system offish 1. Melanophore-concentrating action of potassium and some other ions. Annot. Zool. Jpn.32, 47–58 (1959)Google Scholar
  8. Fujii, R.: A functional interpretation of the fine structure in the melanophore of the guppy,Lebistes reticulatus. Annot. Zool. Jpn.39, 185–192 (1966)Google Scholar
  9. Fujii, R., Novales, R.R.: Cellular aspects of the control of physiological color changes in fishes. Am. Zool.9, 453–463 (1969)Google Scholar
  10. Fujii, R., Taguchi, S.: The responses of fish melanophores to some melanin aggregating and dispersing agents in potassium rich medium. Annot. Zool. Jpn.42, 176–182 (1969)Google Scholar
  11. Geschwind, I.I., Horowitz, J.M., Mikuckis, G.M., Dewey, R.D.: Iontophoretic release of cyclic AMP and dispersion of melanosomes. J. Cell Biol.74, 928–939 (1977)Google Scholar
  12. Gray, E.G.: Control of melanophores of the minnow,Phoxinus phoxinus L. J. Exp. Biol.33, 448–459 (1956)Google Scholar
  13. Green, L.: Mechanisms of movements of granules in melanophores ofFundulus heteroclitus. Proc. Nat. Acad. Sci. (USA)59, 1179–1186 (1968)Google Scholar
  14. Healey, E.G.: The colour change of the minnow (Phoxinus laevis Ag.). I. Effects of spinal section between vertebrae 5 and 12 on the responses of the melanophores. J. Exp. Biol.28, 298–319 (1951)Google Scholar
  15. Healey, E.G.: In: Small animal anaesthesia. Graham-Jones, O. (ed.). London: Pergamon Press 1964Google Scholar
  16. Healey, E.G.: Experimental evidence for the regeneration of nerve fibres controlling colour changes after anterior spinal section in the minnow (Phoxinus phoxinus L.). Proc. Roy. Soc. (B)168, 57–81 (1967)Google Scholar
  17. Hogben, L., Landgrebe, F.W.: The pigmentary effector system. IX. The receptor fields of the teleost visual response. Proc. Roy. Soc. (B)128, 317–342 (1940)Google Scholar
  18. Hogben, L., Slome, D.: The pigmentary effector system VI. The dual character of endocrine co-ordination in amphibian colour change. Proc. Roy. Soc. (B)108, 10–53 (1931)Google Scholar
  19. Iwata, K.S., Watanabe, M., Kurihara, T.: Changes in state and response of the fish scale melanophore during continuous immersion in Ringer's solution. Biol. J. Okayama Univ.5, 185–194 (1959a)Google Scholar
  20. Iwata, K.S., Watanabe, M., Nago, K.: The mode of action of pigment concentrating agents on the melanophores of an isolated fish scale. Biol. J. Okayama Univ.5, 195–206 (1959b)Google Scholar
  21. Iwata, K.S., Yamane, H.: Responses of fish scale melanophores to modification of ionic composition. Biol. J. Okayama Univ.5, 1–11 (1959)Google Scholar
  22. Kamada, T., Kinosita, H.: Movements of granules in fish melanophores. Proc. Jap. Acad.20, 484–492 (1944)Google Scholar
  23. Khokhar, R.: The chromatic physiology of the catfishIctalurus melas (Rafinesque) -I. The melanophore responses of intact and eyeless fish. Comp. Biochem. Physiol.39A, 531–543 (1971)Google Scholar
  24. Kinosita, H.: Studies on the mechanism of pigment migration within fish melanophores with special reference to their electrical properties. Annot. Zool. Jpn.26, 115–127 (1953)Google Scholar
  25. Martin, A.R., Snell, R.S.: A note on the transmembrane potential in the dermal melanophores of the frog and movement of the melanin granules. J. Physiol. (Lond.)195, 755–759 (1968)Google Scholar
  26. Matsushita, K.: Studies on the color change of the catfishParasilurus asotus (L.) Sci. Rept. Tohoku Univ. 4th Series13, 171–200 (1938)Google Scholar
  27. Miyashita, Y.: A preliminary report on the reactivation of pigment movements in Triton-treated model melanophores of fish. J. Sapporo Med. Coll.16, 41–44 (1975)Google Scholar
  28. Murphy, D.B.: The mechanism of microtubule-dependent movement of pigment granules in teleost melanophores. Ann. NY Acad. Sci.253, 692–701 (1975)Google Scholar
  29. Murphy, D.B., Tilney, L.G.: The role of microtubules in the movement of pigment granules in teleost melanophores. J. Cell Biol.61, 757–779 (1974)Google Scholar
  30. Neill, R.M.: On the existence of two types of chromatic behaviour in teleost fishes. J. Exp Biol.17, 74–94 (1940)Google Scholar
  31. Osborn, C.M.: The role of melanophore dispersing principle of the pituitary in the color change of the catfish. J. Exp. Zool.79, 309–331 (1938)Google Scholar
  32. Porter, K.R.: Microtubules in intracellular locomotion. In: Locomotion of tissue cells. Ciba Found. Symp.14, 149–169. Amsterdam: Excerpta Medica 1973Google Scholar
  33. Schliwa, M., Bereiter-Hahn, J.: Pigment movements in fish melanophores: morphological and physiological studies. II. Cell shape and microtubules. Z. Zellforsch.147, 107–125 (1973)Google Scholar
  34. Šećerov, S.: Farbenwechselversuche an der Bartgrundel (Nemachilus barbatulus L.). Arch. Entwicklungsmech. Org.28, 629–660 (1909)Google Scholar
  35. Simpson, G.G., Roe, A., Lewontin, R.C.: Quantitative zoology. New York: Academic Press 1960Google Scholar
  36. Thing, E.: Melanophore reaction and adrenocorticotrophic hormone. I. Comparison of methods based upon photo-electric measurements and microscopic observations. Acta Endocrinol.10, 295–319 (1952)Google Scholar
  37. Tilney, L.G., Bilke, D., Porter, K.R.: Microtubules and pigment migration in the melanophores ofFundulus heteroclitus L. Protoplasma61, 322–345 (1966)Google Scholar
  38. Waring, H.: Color change mechanisms of cold-blooded vertebrates New York: Academic Press 1963Google Scholar
  39. Young, J.Z.: The preparation of isotonic solutions for use in experiments with fish. Pubbl. Staz. Zool. Napoli12, 425–431 (1933)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • C. S. Collis
    • 1
  1. 1.Department of ZoologyBedford College (University of London)Regent's Park, LondonEngland

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