Dark recovery of polarized light sensitive and insensitive receptor cells in the retina of the fly
- 44 Downloads
Dark adaptation of individual receptor cells (R1-R6) was studied in the flyCalliphora erythrocephala. The site of the recordings was identified by intracellular Procion yellow staining. The dark adapted receptor cells were stimulated by 10 μs double delta-flashes from a xenon stroboscope, with different interstimulus intervals (ISI) between 7 and 300 ms. The spectral and polarized light sensitivities of the cells were measured and correlated with the anatomical classification of the cells.
The dark recovery of a cell depends among other things on the measured parameter of the potential, on the intensity of the conditioning flash, and on the polarized light sensitivity of the cell. The full dark recovery of a cell was found in every case to occur at least 100 to 300 ms after the conditioning flash. Of the different parameters which were measured, the amplitude of the potential response gave the slowest and the integral the fastest dark recovery.
Polarized light sensitive cells showed faster recovery and better temporal resolution than polarized light insensitive cells. No relationship could be found between cell morphology and the polarized light sensitivity or dark recovery, i.e., the same morphological cell type was found to belong to different physiological groups.
No relationship between spectral sensitivity and dark recovery could be found.
A possible explanation for the existence of two physiological groups of cells might be found in the occurrence of pigments having different mobility on the membranes of receptor cells or in the coupling of pairs of receptors in the lamina, as suggested by recent electronmicroscopical findings.
KeywordsRetina Temporal Resolution Receptor Cell Spectral Sensitivity Interstimulus Interval
Unable to display preview. Download preview PDF.
- Autrum, H., Kolb, G.: The dark adaptation in single visual cells of the compound eye ofAeschna cyanea. J. comp. Physiol.79, 213–232 (1972)Google Scholar
- Autrum, H., Zwehl, V. v.: Die spektrale Empfindlichkeit einzelner Sehzellen des Bienenauges. Z. vergl. Physiol.48, 357–384 (1964)Google Scholar
- Bernhard, C.G., Ottoson, D.: Studies on the relation between the pigment migration and the sensitivity changes during dark adaptation in diurnal and nocturnal Lepidoptera. J. gen. Physiol.44, 205 (1960)Google Scholar
- Bishop, L.G.: An ultraviolet photoreceptor in a dipteran compound eye. J. comp. Physiol.91, 267–276 (1974)Google Scholar
- Burkhardt, D.: Spectral sensitivity and other response characteristics of single visual cells in the arthropod eye. Symp. Soc. exp. Biol.16, 86–109 (1962)Google Scholar
- Che, Chi, Carlson, S.: High voltage electronmicroscopy of the optic neuropile of the housefly,Musca domesticus. Cell Tiss. Res.167, 537–545 (1976)Google Scholar
- Dudek, F.E., Koopowitz, H.: Adaptation and temporal characteristics of the insect visual response. J. comp. Physiol.82, 33–46 (1973)Google Scholar
- Duncan, G., Croghan, P.G.: Excitation and adaptation in the cephalopod retina: an equivalent circuit model. In: Biochemistry and physiology of visual pigments (ed. H. Langer). Berlin-Heidelberg-New York: Springer 1973Google Scholar
- Fein, A., DeVoe, R.D.: Adaptation in the ventral eye ofLimulus is functionally independent of the photochemical cycle, membrane potential, and membrane resistance. J. gen. Physiol.61, 273–289 (1973)Google Scholar
- Fuortes, M.G.F., O'Bryan, P.M.: Generator potentials in invertebrate photoreceptors. In: Handb. sens. Physiol., Vol. VII/I (ed. M.G.F. Fuortes). Berlin-Heidelberg-New York: Springer 1972Google Scholar
- Hamdorf, K., Kaschef, A.H.: Adaptation beim Fliegenauge. Z. vergl. Physiol.51, 67–95 (1965)Google Scholar
- Horridge, G.A., Mimura, K.: Fly photoreceptors. I. Physical separation of two visual pigments inCalliphora retinula cells 1–6. Proc. Roy. Soc. London Ser. B.190, 211–224 (1975)Google Scholar
- Järvilehto, M., Moring, J.: Polarization sensitivity of individual retinula cells and neurons of the FlyCalliphora. J. comp. Physiol.91, 387–397 (1974)Google Scholar
- Järvilehto, M., Moring, J.: Spectral and polarization sensitivity of identified retinal cells of the fly. In: Neural principles in vision (ed. F. Zettler, F. Weiler). Berlin-Heidelberg-New York: Springer 1976Google Scholar
- Jarvilehto, M., Zettler, F.: Localized intracellular potentials from pre- and postsynaptic components in the external plexiform layer of an insect retina. Z. vergl. Physiol.75, 422–440 (1971)Google Scholar
- Kirschfeld, K.: The possible role of photostable pigments within the membrane of photorecpetors. EMBO-Workshop on “Transduction mechanism of photoreceptors”, Julicn 4.–8 Oct. 1976, pp. 1–9Google Scholar
- Kirschfeld, K., Franceschini, N.: Ein Mechanismus zur Steuerung des Lichtflusses in den Rhabdomeren des Komplexauges vonMusca. Kybernetik6, 13–22 (1969)Google Scholar
- Levick, W.R., Zacks, J.L.: Responses of cat retinal ganglion cells to brief flashes of light. J. Physiol. (Lond.)206, 677–700 (1970)Google Scholar
- Scholes, J.: The electrical responses of the retinal receptors and the lamina in the visual system of the flyMusca. Kybernetik6, 149–162 (1969)Google Scholar
- Weeks, F., Duncan, G.: Photoreception by a cephalopod retina: Response dynamics. Exp. Eye Res.19, 493–500 (1974)Google Scholar
- Zettler, F.: Die Abhängigkeit des Übertragungsverhaltens von Frequenz und Adaptationszustand; gemessen am einzelnen Lichtrezeptor vonCalliphora erythrocephala. Z. vergl. Physiol.64, 432–449 (1969)Google Scholar
- Zettler, F., Järvilehto, M.: Decrement-free conduction of graded potentials along the axon of a monopolar neuron. Z. vergl. Physiol.75, 402–421 (1971)Google Scholar