Journal of comparative physiology

, Volume 143, Issue 4, pp 527–539 | Cite as

Development of the mouse circadian pacemaker: Independence from environmental cycles

  • Fred C. Davis
  • Michael Menaker
Article

Summary

  1. 1.

    The freerunning period (τ) of the circadian pacemaker underlying the wheel-running activity rhythm ofMus musculus was found to be unaffected by the periods of environmental cycles (maternal and light/dark) under which the mice are raised. Mice born to mothers entrained to periods (T) of 28 or 20 h (ratio of light to dark of 14/10) and maintained on those cycle until beyond puberty showed only a temporary difference in freerunning period when placed into constant darkness. Such temporary ‘after-effects’ of entrainment were shown, as had been previously, to occur in animals exposed to non-24-h cycles as adults only.

     
  2. 2.

    After-effects on the ratio of activity to rest (α/ϱ) were not even temporarily different in animals raised onT=28 or T=20.

     
  3. 3.

    Rearing on T=28 or T=20 did not affect the abilities of animals to entrain to these cycles later in life.

     
  4. 4.

    Measurements from young and old animals as well as remeasurement of the young animals later in their lives revealed several effects of age on the pacemaker: a) After-effects on freerunning period after T = 28 or T = 20 are not greater but last longer in older animals; b) Freerunning period is shorter in younger animals; and c) The ratio of activity to rest changes over time in constant darkness and is greater in young animals. Together these suggest that pacemaker ‘plasticity’ reflected in changes in τ and α/ϱ over time in constant darkness decreases with age.

     
  5. 5.

    The length of gestation measured in ‘real’ time was the same in mice entrained to T = 28 or T=20, demonstrating that gestation is not measured in circadian cycles.

     

Keywords

Temporary Difference Young Animal Activity Rhythm Constant Darkness Circadian Cycle 

Abbreviations

LD

light/dark

DD

constant darkness

T

period of the entraining cycle

t

period of the pacemaker

α/ϱ

ratio of activity time to rest time

ANOVA

analysis of variance

SEM

standard error of the mean

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References

  1. Aschoff J (1960) Exogenous and endogenous components in circadian rhythms. Cold Spring Harbor Symp Quant Biol 25:11–28Google Scholar
  2. Aschoff J, Meyer-Lohman J (1954) Angeborene 24-Stunden-Periodik beim Küken. Pflügers Arch 260:170–176Google Scholar
  3. Aschoff J, Gerecke U, Wever R (1967) Desynchronization of human circadian rhythms. Jpn J Physiol 17:450–457Google Scholar
  4. Barr M (1973) Prenatal growth of Wistar rats: circadian periodicity of fetal growth late in gestation. Teratology 7:283–287Google Scholar
  5. Browman LG (1952) Artificial sixteen-hour day activity rhythms in the white rat. Am J Physiol 168:694–697Google Scholar
  6. Brown FM (1974) 27-hour day effects on reproduction and circadian activity period in rats. In: Scheving LE, Halberg F, Pauley JE (eds) Chronobiology. Igoku Shoin, Tokyo, pp 466–471Google Scholar
  7. Daan S, Aschoff J (1975) Circadian rhythms of locomotor activity in captive birds and mammals: their variations with season and latitude. Oecologia 18:269–316Google Scholar
  8. Davis FC, Menaker M (1980) Hamsters through time's window: temporal structure of hamster locomotor rhythmicity. Am J Physiol 239:R149-R155Google Scholar
  9. Deguchi T (1975) Ontogenesis of a biological clock for serotonin: acetylcoenzyme A N-acetyltransferase in pineal gland of rat. Proc Natl Acad Sci USA 72:2814–2828Google Scholar
  10. Fuchs JL, Moore RY (1980) Development of circadian rhythmicity and light responsiveness in the rat suprachiasmatic nucleus: a study using the 2-deoxy[1-14C]glucose method. Proc Natl Acad Sei USA 77:1204–1208Google Scholar
  11. Güldner FH (1978) Synapses of optic nerve afferents in the rat suprachiasmatic nucleus. I. Identification, qualitative description, development and distribution. Cell Tissue Res 194:17–35Google Scholar
  12. Hoffmann K (1957) Angeborene Tagesperiodik bei Eidechsen. Naturwissenschaften 44:359–360Google Scholar
  13. Hoffmann K (1959) Die Aktivitätsperiodik von im 18- und 36- Stundentag erbrüteten Eidechsen. Z Vergl Physiol 42:422–432Google Scholar
  14. Lanman TJ, Seidman L (1977) Length of gestation in mice under a 21-hour day. Biol Reprod 17:224–227Google Scholar
  15. Lenn NJ, Bruce B, Moore RY (1977) Postnatal development of the suprachiasmatic hypothalamic nucleus of the rat. Cell Tissue Res 178:463–475Google Scholar
  16. Mason CA, Sparrow N, Lincoln DW (1977) Structural features of the retinohypothalamic projection in the rat during normal development. Brain Res 132:141–148Google Scholar
  17. Menaker M, Takahashi JS, Eskin A (1978) The physiology of circadian pacemakers. Annu Rev Physiol 40:501–526Google Scholar
  18. Miles LEM, Raynal DM, Wilson MA (1977) Blind man living in normal society has circadian rhythms of 24.9 hours. Science 198:421–423Google Scholar
  19. Moore RY (1973) Retinohypothalamic projection in mammals: a comparative study. Brain Res 49:403–409Google Scholar
  20. Moore RY (1978) Central neural control of circadian rhythms. In: Ganong WF, Martini L (eds) Frontiers in neuroendocrinology, vol 5. Raven Press, New York, p 185Google Scholar
  21. Pittendrigh CS (1954) On temperature independence in the clock system controlling emergence time inDrosophila. Proc Natl Acad Sci USA 40:1018–1029Google Scholar
  22. Pittendrigh CS (1960) Circadian rhythms and the circadian organization of living systems. Cold Spring Harbor Symp Quant Biol 25:159–184Google Scholar
  23. Pittendrigh CS, Daan S (1974) Circadian oscillations in rodents: a systematic increase in their frequency with age. Science 186:548–550Google Scholar
  24. Pittendrigh CS, Daan S (1976a) A functional analysis of circadian pacemakers in nocturnal rodents. I. The stability and lability of spontaneous frequency. J Comp Physiol 106:223–252Google Scholar
  25. Pittendrigh CS, Daan S (1976b) A functional analysis of circadian pacemakers in nocturnal rodents. IV. Entrainment: the clock-like properties of the pacemaker. J Comp Physiol 106:291–331Google Scholar
  26. Pittendrigh CS, Daan S (1976c) A functional analysis of circadian pacemakers in nocturnal rodents. V. Pacemaker structure: a clock for all seasons. J Comp Physiol 106:333–355Google Scholar
  27. Richter CP (1971) Inborn nature of the rat's 24-hour clock. J Comp Physiol Psychol 75:1–4Google Scholar
  28. Rusak B (1979) Neural mechanisms for entrainment and generation of mammalian circadian rhythms. Fed Proc 38:2589–2595Google Scholar
  29. Rusak B, Zucker I (1979) Neural regulation of circadian rhythms. Physiol Rev 59:449–526Google Scholar
  30. Stanfield B, Cowan WM (1976) Evidence for a change in the retino-hypothalamic projection in the rat following early removal of one eye. Brain Res 104:129–136Google Scholar
  31. Sulzman FM, Fuller CA, Hiles LG, Moore-Ede MC (1978) Circadian rhythm dissociation in an environment with conflicting temporal information. Am J Physiol 235:R175-R180Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Fred C. Davis
    • 1
  • Michael Menaker
    • 1
  1. 1.Institute of Reproductive Biology, Department of ZoologyUniversity of TexasAustinUSA

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