Advertisement

European Journal of Clinical Pharmacology

, Volume 12, Issue 3, pp 229–233 | Cite as

Dose dependence of the14C-aminopyrine breath test

Intrasubject comparison of tracer and pharmacological doses
  • Iva Gikalov
  • Johannes Bircher
Originals

Summary

Although the aminopyrine breath test has received much attention, the question has not yet been settled whether pharmacological or tracer doses of the drug should be used. Nine volunteers were given14C-aminopyrine 9 mg/kg or a tracer amount, in a randomized sequence and according to a crossover design. The specific activity of14CO2 in breath was significantly different only during the first hour. Up to the 8th hour the disappearance of14CO2 from breath was smaller after the pharmacological (28.5±SD 5.4%/h) than after the tracer dose (36.2±10.6%/h; p<0.05). The overall disappearance of14C-atoms from the subjects was significantly slower after the higher dose. In view of the smaller radiation exposure and the decreased risk of agranulocytosis, the use of a tracer dose appears preferable.

Key words

Aminopyrine metabolism 14CO2-breath test aminopyrine demethylation dose dependent metabolism physical fitness 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hepner, G.W., Vesell, E.S.: Assessment of aminopyrine metabolism in man by breath analysis after oral administration of14C-aminopyrine. Effects of phenobarbital, disulfiram and portal cirrhosis. New Engl. J. Med.291, 1384–1388 (1974)Google Scholar
  2. 2.
    Hepner, G.W., Vesell, E.S.: Quantitative assessment of hepatic function by breath analysis after oral administration of (14C) aminopyrine. Ann. intern. Med.83, 632–638 (1975)Google Scholar
  3. 3.
    Bircher, J., Küpfer, A., Gikalov, I., Preisig, R.: Aminopyrine demethylation measured by breath analysis in cirrhosis. Clin. Pharmacol. Ther.20, 484–492 (1976)Google Scholar
  4. 4.
    Bircher, J., Platzer, R., Gikalov, I., Küpfer, A., Preisig, R.: Aminopyrine breath test for evaluation of liver function. How to analyse the14CO2 data. Radioaktive Isotope in Klinik und Forschung. Gasteiner Internationales Symposium,12, pp. 347–356 (1976)Google Scholar
  5. 5.
    Winchell, H.S., Stahelin, H., Kusubov, N.: Kinetics of CO2-HCO3 in normal adult males. J. nucl. Med.11, 711–715 (1970)Google Scholar
  6. 6.
    Metzler, C.M.: A user's manual for NONLIN. Technical report 7292/69/7292/005. Kalamazoo, Mich.: The Upjohn Company 1969Google Scholar
  7. 7.
    Lauterburg, B.H., Bircher, J.: Expiratory measurement of maximal aminopyrine demethylation in vivo: effect of phenobarbital, partial hepatectomy, portacaval shunt and bile duct ligation in the rat. J. Pharmacol. exp. Ther.196, 501–509 (1976)Google Scholar
  8. 8.
    La Du B., Gaudette, L., Tronsof, N., Brodie, B.B.: Enzymatic dealkylation of aminopyrine (Pyramidon) and other alkylamines. J. biol. Chem.214, 741–752 (1955)Google Scholar
  9. 9.
    Gram, T.E., Wilson, J.T., Fouts, J.R.: Some characteristics of hepatic microsomal systems which metabolize aminopyrine in the rat and rabbit. J. Pharmacol. exp. Ther.159, 172–181 (1968)Google Scholar
  10. 10.
    Raaflaub, J., Dubach, U.C.: On the pharmacokinetics of phenacetin in man. Europ. J. clin. Pharmacol.8, 261–265 (1975)Google Scholar
  11. 11.
    Brock-Neely, W.: The metabolic fate of formaldehyde14C intraperitoneally administered to the rat. Biochem. Pharmacol.13, 1137–1142 (1964)Google Scholar
  12. 12.
    Brodie, B.B., Axelrod, J.: The fate of aminopyrine (Pyramidon) in man and methods for the estimation of aminopyrine and its metabolites in biological material. J. Pharmacol. exp. Ther.99, 171–184 (1950)Google Scholar
  13. 13.
    Alvares, A.P., Anderson, K.E., Conney, A.H., Kappas, A.: Interactions between nutritional factors and drug biotransformations in man. Proc. Natl. Acad Sci. USA73, 2501–2504 (1976)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Iva Gikalov
    • 1
  • Johannes Bircher
    • 1
  1. 1.Department of Clinical PharmacologyUniversity of BerneBerneSwitzerland

Personalised recommendations