Chemistry of Natural Compounds

, Volume 26, Issue 2, pp 143–147 | Cite as

Fatty acid composition of the main phospholipids of the Antarctic krill Euphausia superba

  • K. Yu. Gordeev
  • V. N. Filarin
  • S. V. Bondarenko
  • M. A. Kirpichenok
  • N. A. Gordeeva
  • I. I. Grandberg
  • S. G. Batrakov
Article

Abstract

The Antarctic krillEuphausia superba Dana contains about 5% of its natural weight of extractable lipids, more than half of which are in the form of phospholipids — phosphatidylcholine (33–36% of the sum of the lipids), phosphatidylethanolamine (15–17%), lysophosphatidylcholine (3–4%), and others (2–3%) — while among the phosphorus-free components triacylglycerols predominate (32–35%). In the first two phospholipids the dominating fatty acid residue is the arachidonic acid residue (more than 40% of the sum of the acyl residues) and the amounts of residues of eicosapentaenoic acid (C20 : 5w3) are about 13 and 28%, respectively. A simple procedure for isolating the phosphatidylcholine and phosphatidylethanolamine is proposed. For the analysis of the fatty acid a new method was used, consisting of the HPLC of their (7-diethylaminocoumarin-4-yl)hydrazides, with fluorometric detection.

Keywords

Lipid Acid Composition Fatty Acid Composition Arachidonic Acid Phosphatidylcholine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    V. K. S. Shukla, J. Clausen, H. Egagaard, and E. Larsson, Fette, Seifen, Anstrichm.,82, 193 (1980); J. Dyerberg and K. A. Jorgensen, Prog. Lipid Res.,21, 255 (1982); G. G. Bruckner, B. Lokesh, B. German and J. E. Kinsella, Thromb. Res.,34, 479 (1984); G. J. Nelson and R. G. Ackman, Lipids,23, No. 11, 1005 (1988).Google Scholar
  2. 2.
    H. Fricke, G. Gercken, W. Schreiber, and J. Oehlenschläger, Lipids,19, No. 11, 821 (1984).Google Scholar
  3. 3.
    N. R. Bottino, Comp. Biochem. Physiol.,50B, No. 3, 479 (1975); T. E. Ellingson, and T. E. Mohr, in: 11th Scandanavian Symposium on Lipids, 1981, Marcus, Goeteborg (1982), p. 110; F. M. Rzhavskaya, E. A. Sakaeva, and T. A. Dubrovskaya, Krill Processing Technology. A Collection of Scientific Papers [in Russian], VNIRO, Moscow (1981), p. 24.Google Scholar
  4. 4.
    O. A. Artyukova, The Lipids of the Antarctic Krill (Euphausia superba Dana) and Their Therapeutic Effect in Experimental Hypercholesteremia [in Russian], Inst. Pitanya AMN SSSR, Moscow (1988).Google Scholar
  5. 5.
    J. Folch, M. Lees, and G. H. Sloane-Stanley, J. Biol. Chem.,226, No. 2, 497 (1957).Google Scholar
  6. 6.
    S. G. Batrakov, G. A. Ermolin, V. A. Vnukov, O. G. Sakandelizdze, and T. K. Mitrofanova, USSR Inventor's Certificate No. 1102603; Byull. Izobret., No. 26 (1984).Google Scholar
  7. 7.
    V. M. Dembitskii, Ukr. Biokhim. Zh.,59, No. 1, 50 (1987).Google Scholar
  8. 8.
    S. Nobukazu, Nippon Suisan Gakkashi,49, No. 2, 259 (1983); H. Fricke, G. Gercken, and J. Oehlenschläger, Comp. Biochem. Physiol.,85B, No. 1, 131 (1986).Google Scholar
  9. 9.
    O. Saether, T. E. ELlingson, and V. Mohr, J. Lipid Res.,27, 274 (1986).Google Scholar
  10. 10.
    H. Miwa, M. Yamamoto, and T. Nishida, Clin. Clim. Acta,155, No. 1, 95 (1986).Google Scholar
  11. 11.
    V. E. Vaskovsky [Vaskovskii] and V. I. Svetashev, J. Chromatogr.,65, 451 (1972).Google Scholar
  12. 12.
    J. G. Kirchner, Thin Layer Chromatography, Wiley-Interscience, New York, 2nd edn., (1978) [Russian translation, Mr. Moscow (1981), Ch. 7].Google Scholar
  13. 13.
    L. D. Bergelson, E. V. Djatlovitskaja [Dyatlovitskaya], and V. V. Voronkova, J. Chromatogr.,15, 191 (1964).Google Scholar
  14. 14.
    L. D. Bergel'son, E. V. Dyatlovitskaya, Yu. G. Molotkovskii, S. G. Batrakov, L. I. Barsukov, and N. V. Prokazova, The Preparative Biochemistry of Lipids [in Russian], Nauka, Moscow (1981).Google Scholar
  15. 15.
    M. Kates, Techniques of Lipidology, American Elsevier, New York (1972).Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • K. Yu. Gordeev
  • V. N. Filarin
  • S. V. Bondarenko
  • M. A. Kirpichenok
  • N. A. Gordeeva
  • I. I. Grandberg
  • S. G. Batrakov

There are no affiliations available

Personalised recommendations