Cellular identification of the violet receptor in the crayfish eye
Ten violet receptors in the retinas of crayfish (Procambarus) were injected intracellularly with the fluorescent dye Lucifer Yellow-CH and subsequently identified in histological preparations. All had their cell body located distal to the main rhabdom, in the position of the small, 8th retinular cell. In nine cases it was possible to trace the axon of the violet receptor beyond thelamina ganglionaris, and in four cases, to its termination in themedulla.
By contrast, 22 green receptors similarly injected were all found to contribute to the main rhabdom, which is formed by retinular cells 1–7. Their axons synapsed in thelamina ganglionaris.
Microspectrophotometry of the 8th cell reveals an absorption peak at 440 nm. As previous microspectrophotometric observations indicated that retinular cells 1–7 all contain a visual pigment with λmax at 530 nm, the microspectrophotometric data confirm that the violet receptor is cell 8.
KeywordsRetina Absorption Peak Cell Body Visual Pigment Histological Preparation
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- Eguchi E (1965) Rhabdom structure and receptor potentials in single crayfish retinular cells. J Cell Comp Physiol 66:411–430Google Scholar
- Eguchi E, Waterman TH, Akiyama J (1973) Localization of the violet and yellow receptor cells in the crayfish retinula. J Gen Physiol 62:355–374Google Scholar
- Goldsmith TH (1978a) The spectral absorption of crayfish rhabdoms: pigment, photoproduct and pH sensitivity. Vision Res 18:463–473Google Scholar
- Goldsmith TH (1978b) The effects of screening pigments on the spectral sensitivity of some Crustacea with scotopic (superposition) eyes. Vision Res 18:475–482Google Scholar
- Goldsmith TH, Fernández HR (1968) Comparative studies of crustacean spectral sensitivity. Z Vergl Physiol 60:156–175Google Scholar
- Hanström B (1927) Über die Frage, ob funktionell-verschiedene, zapfen- und stäbchenartige Sehzellen im Komplexauge der Arthropoden vorkommen. Z Vergl Physiol 6:566–597Google Scholar
- Harreveld AD van (1932) A physiological solution for freshwater crustaceans. Proc Soc Exp Biol New York 34:428–432Google Scholar
- Harris WA, Stark W, Walker JA (1976) Genetic dissection of the photoreceptor system in the compound eye ofDrosophila melanogaster. J Physiol (Lond) 256:415–439Google Scholar
- Kong K-L, Goldsmith TH (1977) Photosensitivity of retinular cells in white-eyed crayfish (Procambarus clarkii) J Comp Physiol 122:273–288Google Scholar
- Menzel R, Blakers M (1976) Colour receptors in the bee eye — morphology and spectral sensitivity. J Comp Physiol 108:11–33Google Scholar
- Nässei DR (1976) The retina and retinal projection on the lamina ganglionaris of the crayfishPacifastacus leniusculus (Dana). JComp Neurol 167:341–360Google Scholar
- Nässei DR (1977) Types and arrangement of neurons in the crayfish optic lamina. Cell Tissue Res 179:45–75Google Scholar
- Nosaki H (1969) Electrophysiological study of color encoding in the compound eye of crayfish,Procambarus clarkii. Z Vergl Physiol 64:318–323Google Scholar
- Wald G (1968) Single and multiple visual systems in arthropods. J Gen Physiol 51:125–156Google Scholar
- Waterman TH, Fernández HR (1970) E-vector and wavelength discrimination by retinular cells of the crayfishProcambarus. Z Vergl Physiol 68:154–174Google Scholar
- Waterman TH, Pooley AS (1980) Crustacean fine structure seen with scanning electron microscopy. Science 209:235–240Google Scholar
- Waterman TH, Fernández HR, Goldsmith TH (1969) Dichroism of photosensitive pigment in rhabdoms of the crayfishOrconectes. J Gen Physiol 54:415–432Google Scholar