Journal of Comparative Physiology A

, Volume 154, Issue 2, pp 175–187 | Cite as

Non-local interactions between light induced processes inCalliphora photoreceptors

  • Baruch Minke
  • Kuno Kirschfeld


The prolonged depolarizing afterpotential (PDA) is a phenomenon which is tightly linked to visual pigment conversion. In order to determine whether processes underlying PDA induction and depression can spread in space, the PDA was recorded intracellularly in white-eyedCalliphora R1-6 photoreceptors and used to examine interactions between processes induced by activating statistically different photopigment molecules (Figs. 3–6). It was found that a PDA induced by converting some fraction of rhodopsin (R) molecules forward into the metarhodopsin (M) state can be completely depressed by equal or smaller amounts of pigment conversion, backward from metarhodopsin to rhodopsin even when largely different sets of pigment molecules were shifted in the respective directions, in agreement with previous experiments conducted on the barnacle. The characteristics of the afterpotentials obtained following the cessation of strong blue and green light stimuli which did not cause a net pigment conversion was examined (Figs. 7, 8). It was found that these afterpotentials, obtained when nonet R to M conversion took place, could not be depressed by an opposite net large M to R pigment conversion. Accordingly we propose to restrict the term PDA to an afterpotential which can be depressed by a net M to R pigment conversion. It is concluded: (a) that some processes underlying PDA induction and depression inCalliphora must interact at a distance which extends at least to the nearest neighboring pigment molecule, and (b) that inCalliphora photoreceptors net pigment conversion is required in order to induce and depress a PDA.


Depression Previous Experiment Light Stimulus Induce Process Visual Pigment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.






R to M

rhodopsin to metarhodopsin pigment conversion

M to R

metarhodopsin to rhodopsin pigment conversion


prolonged depolarizing afterpotential



M potential

metarhodopsin potential


early receptor potential


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  1. Albani C, Nöll GN, Yoshikami S (1980) Rhodopsin regeneration, calcium, and the control of the dark current in vertebrate rods. Photochem Photobiol 32:515–520Google Scholar
  2. Armon E, Minke B (1983) Light activated electrogenic Na+- Ca+2 exchange in fly photoreceptors: Modulation by Na+/ K+-pump activity. Biophys Struct Mech 9:349–357Google Scholar
  3. Baumann F, Hadjilazaro B (1972) A depolarizing aftereffect of intense light in the drone visual receptor. Vision Res 12:17–31Google Scholar
  4. Brown JE, Lisman JE (1972) An electrogenic sodium pump inLimulus ventral photoreceptor cells. J Gen Physiol 59:720–733Google Scholar
  5. Hamdorf K (1979) The physiology of invertebrate visual pigments. In: Autrum H (ed) Handbook of sensory physiology, vol VII/6 A. Springer, Berlin Heidelberg New York, pp 145–224Google Scholar
  6. Hamdorf K, Razmjoo S (1977) The prolonged depolarizing afterpotential and its contribution to the understanding of photoreceptor function. Biophys Struct Mech 3:163–170Google Scholar
  7. Hamdorf K, Razmjoo S (1979) Photoconvertible pigment states and excitation inCalliphora; the induction and properties of the prolonged depolarizing afterpotential. Biophys Struct Mech 5:137–161Google Scholar
  8. Hillman P, Hochstein S, Minke B (1976) Nonlocal interactions in the photoreceptor transduction process. J Gen Physiol 68:227–245Google Scholar
  9. Hillman P, Hochstein S, Minke B (1983) Transduction in invertebrate photoreceptors: Role of pigment bistability. Physiol Rev 63:668–772Google Scholar
  10. Hochstein S, Minke B, Hillman P (1973) Antagonistic components of the late receptor potential in the barnacle photoreceptor arising from different stages of the pigment process. J Gen Physiol 62:105–128Google Scholar
  11. Ikeda K, Kaplan WD (1970) Patterned neural activity of a mutantDrosophila melanogaster. Proc Natl Acad Sci USA 66:765–772Google Scholar
  12. Koike H, Brown HM, Hagiwara S (1971) Hyperpolarization of a barnacle photoreceptor membrane following illumination. J Gen Physiol 57:723–737Google Scholar
  13. Larrivee DC, Conrad SK, Stephenson RS, Pak WL (1981) Mutation that selectively affects rhodopsin concentration in the peripheral photoreceptors ofDrosophila melanogaster. J Gen Physiol 78:521–545Google Scholar
  14. Minke B (1979) Transduction in photoreceptors with bistable pigments: intermediate processes. Biophys Struct Mech 5:163–174Google Scholar
  15. Minke B, Kirschfeld K (1979) The contribution of a sensitizing pigment to the photosensitivity spectra of fly rhodopsin and metarhodopsin. J Gen Physiol 73:517–540Google Scholar
  16. Minke B, Kirschfeld K (1980) Fast electrical potentials arising from activation of metarhodopsin in the fly. J Gen Physiol 75:381–402Google Scholar
  17. Pak WL, Lidington KJ (1974) Fast electrical potential from a long-lived long-wavelength photoproduct of fly visual pigment. J Gen Physiol 63:740–756Google Scholar
  18. Razmjoo S, Hamdorf K (1980) In support of the ‘photopigment model’ of vision in invertebrates. J Comp Physiol 135:209–215Google Scholar
  19. Stark WS, Zitzmann WG (1976) Isolation of adaptation mechanisms and photopigment spectra by vitamin A deprivation inDrosophila. J Comp Physiol 105:15–27Google Scholar
  20. Stephenson RS, Pak WL (1980) Heterogenic components of a fast electrical potential inDrosophila compound eye and their relation to visual pigment photoconversion. J Gen Physiol 75:353–379Google Scholar
  21. Tsukahara Y, Horridge GA (1977) Miniature potentials, light adaptation and afterpotentials in locust retinula cells. J Exp Biol 68:137–149Google Scholar
  22. Tsukahara Y, Horridge GA (1978) The distribution of bumps in the tail of the locust photoreceptor afterpotential. J Exp Biol 73:1–14Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Baruch Minke
    • 1
    • 2
  • Kuno Kirschfeld
    • 1
    • 2
  1. 1.Department of PhysiologyThe Hebrew University - Hadassah, Medical SchoolJerusalemIsrael
  2. 2.Max-Planck-Institut für biologische KybernetikTübingenGermany

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