Applied Microbiology and Biotechnology

, Volume 33, Issue 6, pp 652–656 | Cite as

Multiple fruiting of the edible mushroomCoprinus cinereus from explants of the stipe

  • Pamela J. Weathers
  • Jeff Blanchard


Carpophores were developed on defined medium from explants of stipes produced in the dark using an expansionless mutant (no. 190) ofCoprinus cinereus. Explants taken from stipes within 24 h of formation developed multiple fruit within 3–12 days without formation of mycelia. Fruiting levels were affected by stipe age (24 h), medium composition, explant size, and polarity of the explant on fruiting medium. This method offers a new tool for developmental studies and may also be of use to commercial mushroom growers.


Medium Composition Developmental Study Mushroom Grower Explant Size Commercial Mushroom 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Pamela J. Weathers
    • 1
  • Jeff Blanchard
    • 1
  1. 1.Department of Biology and BiotechnologyWorcester Polytechnic InstituteWorcesterUSA

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