Summary
Carpophores were developed on defined medium from explants of stipes produced in the dark using an expansionless mutant (no. 190) ofCoprinus cinereus. Explants taken from stipes within 24 h of formation developed multiple fruit within 3–12 days without formation of mycelia. Fruiting levels were affected by stipe age (24 h), medium composition, explant size, and polarity of the explant on fruiting medium. This method offers a new tool for developmental studies and may also be of use to commercial mushroom growers.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballou LR, Holton RW (1985) Synchronous initiation and sporulation of fruit bodies byCoprinus cinereus on defined medium. Mycologia 77:103–108
Gooday GW (1974) Control of development of excised fruit bodies and stipes ofCoprinus cinereus. Trans Br Mycol Soc 62:391–99
Gooday GW (1982) Metabolic control of fruit body morphogenesis inCoprinus cinereus. In: Wells K, Wells E (eds) Basidium and basidiocarp evolution, cytology, function and development, Springer, Berlin, Heidelberg, New York, pp 157–173
Hayes WA, Randle PE, Last FT (1969) The nature of the microbial stimulus affecting sporophore formation inAgaricus bisporus (Lange) Sing. Ann Appl Biol 64:177–87
Lu BC (1974) Meiosis inCoprinus. V. The role of light on basidiocarp initiation, mitosis and hymenium differentiation inCoprinus lagopus. Can J Bot 52:299–306
Madelin MF (1956) The nutrition ofCoprinus lagopus especially as affecting fruiting. Ann Bot 20:307–330
Matthews TR, Niederpruen DJ (1972) Differentiation inCoprinus lagopus. I. Control of fruiting and cytology of initial events. Arch Mikrobiol 87:257–268
Moore D (1984) Developmental biology of theCoprinus cinereus carpophore: metabolic regulation in relation to cap morphogenesis. Exp Mycol 8:283–297
Pukkila PJ, Moore D (1985)Coprinus cinereus: an ideal organism for studies of genetics and developmental biology. J Biol Educ 19:1–40
Taber WA (1966) Morphogenesis in basidiomycetes. In: Ainsworth GC, Sussman AS (eds) The fungi, an advanced treatise, vol 2. Academic Press, New York, pp 387–412
Tautorus TE, Townsley PE (1984) Biotechnology in commercial mushroom fermentation. Bio/Technology 2:696–701
Tsusue YM (1969) Experimental control of fruit-body formation inCoprinus macrorhizus. Dev Growth & Differ 11:64–177
Uno I, Ishikawa T (1984) Effect of glucose on the fruiting body formation and adenosine 3′:5′-cyclic monophosphate levels inCoprinus macrorhizus. J Bacteriol 120:94–100
Uno I, Yamaguchi M, Ishikawa T (1974) The effect of light on fruiting body formation and adenosine 3′:5′-cyclic monophosphate metabolism inCoprinus macrorhizus. Proc Natl Acad Sci USA 71:479–83
Webster J (1980) Introduction to the fungi. Cambridge University Press, New York, pp 423–426
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weathers, P.J., Blanchard, J. Multiple fruiting of the edible mushroomCoprinus cinereus from explants of the stipe. Appl Microbiol Biotechnol 33, 652–656 (1990). https://doi.org/10.1007/BF00604932
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00604932