Archiv für dermatologische Forschung

, Volume 249, Issue 3, pp 247–254 | Cite as

Epidermodysplasia verruciformis

III. Ultraviolet Micro-Irradiation of Cells in Culture
  • G. Moreno
  • M. Pruniéras


Ultraviolet (UV) micro-irradiation of the cell nucleus has been used to study the unscheduled DNA synthesis in cultured fibroblasts of 2 patients withEpidermodysplasia verruciformis (EV).

Cells were micro-irradiated over a nuclear area of 5 μm in diameter with UV doses of the order of 10, 100 and 1000 ergs/mm2 at 275 nm. The unscheduled DNA synthesis in fibroblasts originated from normal and malignant skin was compared with the unscheduled synthesis in threeXeroderma pigmentosum (XP) cell lines defective for unscheduled synthesis.

In cells not in S phase labelled with3H-thymidine, the uptake was localized over the irradiated area. No difference in unscheduled DNA synthesis could be detected with this technique in fibroblasts derived from clinically normal skin versus malignant EV lesions, nor was there any difference as compared to normal control cells (KB and WI 38).

This confirms preliminary data on one first patient indicating that DNA repair following UV irradiation is not impaired in EV.


Normal Control Cell Nucleus Preliminary Data Normal Skin Irradiate Area 


„Unscheduled“ DNA-Synthese wurde nach Ultraviolett-Micro-Irradiation des Fibroblasten-Zellnucleus bei 2 Patienten mit Epidermodysplasia verruciformis (EV) untersucht.

Die Zellkerne wurden über eine Fläche von 5 μm Durchmesser mit UV-Dosen in der Höhe von 10, 100 und 1000 ergs/mm2 bei 275 nm bestrahlt. Fibroblasten aus normaler und carcinomatös veränderter Haut wurden mit drei Zellstämmen von Patienten mit Xeroderma pigmentosum (XP), bei denen die DNA-Synthese gestört verlief, verglichen.

Zellen, die sich nicht in der S-Phase befanden, zeigten eine Thymidin-Aufnahme über dem Bestrahlungsareal. Ein Unterschied zwischen Fibroblasten von klinisch normaler Haut bei EV wie auch von carcinomatös erkrankter Haut konnte hinsichtlich der „unscheduled“ DNA-Synthese nicht gefunden werden. Gleichfalls fand sich kein Unterschied zu normalen Kontrollzellstämmen (KB und WI 38).

Diese Untersuchungen unterstützen frühere Befunde an einem Patienten, bei dem eine UV-abhängige DNA-Synthese bei EV nicht gestört ist.


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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • G. Moreno
    • 1
  • M. Pruniéras
    • 2
  1. 1.Institut de Pathologie Cellulaire, Unité I. N. S. E. R. M. 48Hôpital de BicêtreLe Kremlin-BicêtreFrance
  2. 2.Laboratoire de Recherches sur les Tumeurs de la Peau HumaineFondation Ad. de RothschildParisFrance

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