Advertisement

Archiv für dermatologische Forschung

, Volume 249, Issue 3, pp 247–254 | Cite as

Epidermodysplasia verruciformis

III. Ultraviolet Micro-Irradiation of Cells in Culture
  • G. Moreno
  • M. Pruniéras
Article

Summary

Ultraviolet (UV) micro-irradiation of the cell nucleus has been used to study the unscheduled DNA synthesis in cultured fibroblasts of 2 patients withEpidermodysplasia verruciformis (EV).

Cells were micro-irradiated over a nuclear area of 5 μm in diameter with UV doses of the order of 10, 100 and 1000 ergs/mm2 at 275 nm. The unscheduled DNA synthesis in fibroblasts originated from normal and malignant skin was compared with the unscheduled synthesis in threeXeroderma pigmentosum (XP) cell lines defective for unscheduled synthesis.

In cells not in S phase labelled with3H-thymidine, the uptake was localized over the irradiated area. No difference in unscheduled DNA synthesis could be detected with this technique in fibroblasts derived from clinically normal skin versus malignant EV lesions, nor was there any difference as compared to normal control cells (KB and WI 38).

This confirms preliminary data on one first patient indicating that DNA repair following UV irradiation is not impaired in EV.

Keywords

Normal Control Cell Nucleus Preliminary Data Normal Skin Irradiate Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

„Unscheduled“ DNA-Synthese wurde nach Ultraviolett-Micro-Irradiation des Fibroblasten-Zellnucleus bei 2 Patienten mit Epidermodysplasia verruciformis (EV) untersucht.

Die Zellkerne wurden über eine Fläche von 5 μm Durchmesser mit UV-Dosen in der Höhe von 10, 100 und 1000 ergs/mm2 bei 275 nm bestrahlt. Fibroblasten aus normaler und carcinomatös veränderter Haut wurden mit drei Zellstämmen von Patienten mit Xeroderma pigmentosum (XP), bei denen die DNA-Synthese gestört verlief, verglichen.

Zellen, die sich nicht in der S-Phase befanden, zeigten eine Thymidin-Aufnahme über dem Bestrahlungsareal. Ein Unterschied zwischen Fibroblasten von klinisch normaler Haut bei EV wie auch von carcinomatös erkrankter Haut konnte hinsichtlich der „unscheduled“ DNA-Synthese nicht gefunden werden. Gleichfalls fand sich kein Unterschied zu normalen Kontrollzellstämmen (KB und WI 38).

Diese Untersuchungen unterstützen frühere Befunde an einem Patienten, bei dem eine UV-abhängige DNA-Synthese bei EV nicht gestört ist.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bootsma, D., Mulder, M. P., Pot, F., Cohen, J. A.: Different inherited level of DNA repair replication in Xeroderma pigmentosum cell strains after exposure to ultraviolet irradiation. Mutation Res.9, 507–516 (1970)Google Scholar
  2. Cleaver, J. E.: Defective repair replication of DNA in Xeroderma pigmentosum. Nature (Lond.)218, 652–656 (1968)Google Scholar
  3. Cleaver, J. E.: Xeroderma pigmentosum: a human disease in which an initial stage of DNA repair is defective. Proc. nat. Acad. Sci. (Wash.)63, 428–435 (1969)Google Scholar
  4. Delescluse, C., Pruniéras, M., Régnier, M., Moreno, G., Arouete, J.: Epidermodysplasia verruciformis. I. Electron microscope autoradiography and tissue culture studies. Arch. Derm. Forsch.242, 202–215 (1972)Google Scholar
  5. Delescluse, C., Régnier, M., Pruniéras, M.: Epidermodysplasia verruciformis. II. Uridine incorporation. Arch. Derm. Forsch.247, 89–97 (1973)Google Scholar
  6. Epstein, W. L., Fukuyama, K., Epstein, J. H.: Ultraviolet light, DNA repair and skin carcinogenesis in man. Fed. Proc.30, 1766–1771 (1971)Google Scholar
  7. Epstein, J., Williams, J. R., Little, J. B.: Deficient DNA repair in human progeroid cells. Proc. nat. Acad. Sci. (Wash.)70, 977–881 (1973)Google Scholar
  8. Ham, R. G.: Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. nat. Acad. Sci. (Wash.)53, 288–293 (1965)Google Scholar
  9. Moreno, G., Vinzens, F.: Effects de la micro-irradiation ultraviolette sur différentes parties de la cellule. I. Etude en microscopie électronique sur coupes en série transversales et sagittales. Exp. Cell Res.56, 75–83 (1969)Google Scholar
  10. Moreno, G.: Effects of ultraviolet micro-irradiation on different parts of the cell. II. Cytological observations and unscheduled DNA synthesis after partial nuclear irradiation. Exp. Cell Res.65, 129–139 (1971)Google Scholar
  11. Moreno, G.: Techniques of partial cell irradiation with conventional and laser sources. In: Techniques of biochemical and biophysical morphology, D. Glick and R. M. Rosenbaum, eds., Vol. I, pp. 47–66. New York: J. Wiley 1972Google Scholar
  12. Moreno, G., Salet, C.: Unscheduled DNA synthesis after ultraviolet mino-irradiation of the cell nucleus. Radiat. Res. (in press, 1974)Google Scholar
  13. Painter, R. B.: The action of ultraviolet light on mammalian cells. Photophysiology5, 169–189 (1970)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • G. Moreno
    • 1
  • M. Pruniéras
    • 2
  1. 1.Institut de Pathologie Cellulaire, Unité I. N. S. E. R. M. 48Hôpital de BicêtreLe Kremlin-BicêtreFrance
  2. 2.Laboratoire de Recherches sur les Tumeurs de la Peau HumaineFondation Ad. de RothschildParisFrance

Personalised recommendations