Archiv für dermatologische Forschung

, Volume 241, Issue 1, pp 15–21 | Cite as

Immunofluorescent studies on circulating lymphocytes in oral mucosal diseases

  • K. Sallay
  • P. Dán
  • P. Geck
  • G. Kulcsár
  • I. Nász


Circulating lymphocytes of patients with recurrent herpetic, aphthous and other oral mucosal diseases, as well as of persons with intact oral mucosa were examined with direct immunofluorescence method using HVH and adenovirus hyperimmune conjugated sera. Some of the circulating lymphocytes showed a specific fluorescence both in patients and in persons with intact oral mucosa. Increased herpes-positivity was observed only in erythema exsudativum multiforme and increased adeno-positivity in patients with recurrent aphthous stomatitis, especially in cases with constantly recurring aphthae.

The possible pathologic role of the circulating virus-antigen-carrier lymphocytes is presumed.


Public Health Oral Mucosa Stomatitis Immunofluorescent Study Erythema Multiforme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Es wurden Untersuchungen mit der direkten Immunfluorescenzmethode bei Kranken mit rezidivierenden herpetischen, aphthösen und anderen Mundschleimhautkrankheiten mit Antiherpes- und Antiadenoviruskonjugaten durchgeführt. Sowohl bei den Patienten wie bei den Personen mit intakter Mundschleimhaut wurde eine spezifische Fluorescenz beobachtet. Erhöhte Adenopositivität wurde bei Patienten mit rezidivierenden, besonders mit ständig rezidivierenden Aphthen, erhöhte Herpespositivität bei Kranken mit Erythema multiforme beobachtet.

Die mögliche pathologische Rolle der virusantigen-haltenden Lymphocyten wurde angenommen.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Burnet, F. M.: The concept of autoimmunity. Proc. 11th Congr. int. Soc. Blood. Transf. Sydney 1966. Bibl. haemat. (Basel)29, 423–435 (1968).Google Scholar
  2. 2.
    Clinical stoff conference. The circulating lymphocyte. Its role in infectious mononucleosis. Moderator: L. N. Chessin. Ann. intern. Med.69, 333–359 (1968).Google Scholar
  3. 3.
    Cooke, B. E. D.: The diagnosis of bullous lesions affecting the oral mucosa. Brit. dent. J.109, 83–96 (1960).Google Scholar
  4. 4.
    Coons, A. B., Kaplan, M. H.: Localisation of antigen in tissue cells: improvement in methode for detection of antigen by means of fluorescent antibody. J. exp. Med.91, 1–13 (1950).Google Scholar
  5. 5.
    Dán, P., Sallay, K., Geck, P., Kulcsár., Nász, I.: Demonstration of virusantigens from aphthous and herpetic oral diseases (in Hungarian). Fogorv. Szle63, 249–251 (1970).Google Scholar
  6. 6.
    Dreizin, R. S., Zolotarskaja, E. E., Davidova, A. A.: Immunological structure of the population of Moskow in relation to adenovirus. Vop. Virus.7, 85–91 (1962).Google Scholar
  7. 7.
    Fichtelius, K. E., Groth, O., Lidén, S.: The skin, a first level lymphoid organ? Int. Arch. Allergy.37, 607–620 (1970).Google Scholar
  8. 8.
    Geck, P., Dán, P., Nász, I.: Examination of the cytopathic effect of adenoviruses by immunofluorescence. Acta microbiol. Acad. Sci. hung.11, 19–22 (1964).Google Scholar
  9. 9.
    Gresser, I., Lang, D. J.: Relationship between viruses and leukocytes. Progr. med. Virol.8, 62–130 (1966).Google Scholar
  10. 10.
    Hanshaw, J. B.: Viruses in circulating lymphocytes. New Engl. J. Med.280, 1353–1354 (1969).Google Scholar
  11. 11.
    Kaufman, H. E., Brown, D. C., Ellison, E. M.: Recurrent herpes in the rabbit and man. Science156, 1628–1629 (1967).Google Scholar
  12. 12.
    Kulcsár, G., Sallay, K., Nász, I., Dán, P., Geck, P.: Virusisolierungs-Experimente aus aphthösen Mundschleimhaut-Erkrankungen. Zbl. Bakt., I. Abt. Orig.213, 455–461 (1970).Google Scholar
  13. 13.
    Mikulicz, J., Michelson, P.: Atlas der Krankheiten der Mund- und Rachenhöhle. Berlin: Hirschwald 1891.Google Scholar
  14. 14.
    Munk, K., Runnebaum, H.: Virusinfektionen nach Organtransplantationen bei immunsuppressiver Therapie. Dtsch. med. Wschr.95, 2252–2254 (1970).Google Scholar
  15. 15.
    Nász, I., Lengyel, A., Dán, P., Kulcsár, G.: Informative studies on the haemagglutination spectra of adenoviruses. Acta microbiol. Acad. Sci. hung.9, 69–76 (1962).Google Scholar
  16. 16.
    Nász, I., Tóth, M.: Frequency distribution of the complement fixing antibodies to adenoviruses in sevral group of the Hungarian population. Acta microbiol. Acad. Sci. hung.6, 203–207 (1959).Google Scholar
  17. 17.
    —, —: Nachweis von komplementbindenden und neutralisierenden Antikörpern gegen Adenoviren. Zbl. Bakt., I. Abt. Orig.178, 141–148 (1960).Google Scholar
  18. 18.
    Rawls, W. E.: Congenital rubella: the significance of virus persistence. Progr. med. Virol.10, 238–285 (1968).Google Scholar
  19. 19.
    Rustigian, R., Smulow, J. B., Tyl, M., Gibson, W. A., Shindell, E.: Studies on latent infection of skin and oral mucosa in individuals with recurrent herpes simplex. J. invest. Derm.47, 218–221 (1966).Google Scholar
  20. 20.
    Sallay, K.: Le role du traumatisme dans la production des aphtes recidivants. Acta stomat. belg.67, 273–280 (1970).Google Scholar
  21. 21.
    -- Dán, P., Kulcsár, G., Nász, I.: Transformation of lymphocytes from patients with recurrent aphthae. Rev. Immunol. (in press).Google Scholar
  22. 22.
    Shields, J. W., Touchon, R. C., Dickson, D. R.: Quantitativ studies on small lymphocyte disposition in epithelial cells. Amer. J. Path.54, 129–144 (1969).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • K. Sallay
    • 1
    • 2
  • P. Dán
    • 1
    • 2
  • P. Geck
    • 1
    • 2
  • G. Kulcsár
    • 1
    • 2
  • I. Nász
    • 1
    • 2
  1. 1.Institute of Oral and Maxillo-facial SurgerySemmelweis University of MedicineBudapestHungary
  2. 2.Institute of MicrobiologySemmelweis University of MedicineBudapestHungary

Personalised recommendations